O31611 (YJBM_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP pyrophosphokinase YjbM
EC=2.7.6.5

Alternative name(s):
(p)ppGpp synthase YjbM
Small alarmone synthase 1
Short name=SAS 1

Gene names

Name:	yjbM
Ordered Locus Names:	BSU11600

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. YjbM has probably a minor role in stringent response. Ref.2
Catalytic activity	ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.
Pathway	Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.
Induction	Expressed during exponential growth through the transition to the stationary phase, but not during entry of cells into stationary phase. Ref.2
Miscellaneous	GDP can be used instead of GTP, resulting in an increase of (p)ppGpp synthesis. The synthase activity of YjbM is greater than that of YwaC.
Sequence similarities	Belongs to the RelA/SpoT family.

Ontologies

Keywords
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	guanosine tetraphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP diphosphokinase activity Inferred from electronic annotation. Source: EC kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	GTP pyrophosphokinase YjbM		PRO_0000390876

Sequences

	Sequence		Length	Mass (Da)
	O31611 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 8FB2A73CE865FAA2	FASTA	211	24,704
	10 20 30 40 50 60 MDDKQWERFL VPYRQAVEEL KVKLKGIRTL YEYEDDHSPI EFVTGRVKPV ASILEKARRK 70 80 90 100 110 120 SIPLHEIETM QDIAGLRIMC QFVDDIQIVK EMLFARKDFT VVDQRDYIAE HKESGYRSYH 130 140 150 160 170 180 LVVLYPLQTV SGEKHVLVEI QIRTLAMNFW ATIEHSLNYK YSGNIPEKVK LRLQRASEAA 190 200 210 SRLDEEMSEI RGEVQEAQAA FSRKKKGSEQ Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[2]	"Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis." Nanamiya H., Kasai K., Nozawa A., Yun C.S., Narisawa T., Murakami K., Natori Y., Kawamura F., Tozawa Y. Mol. Microbiol. 67:291-304(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A (P)PPGPP SYNTHASE, INDUCTION. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL009126 Genomic DNA. Translation: CAB13017.1.
PIR	E69844.
RefSeq	NP_389042.1. NC_000964.3.
3D structure databases
ProteinModelPortal	O31611.
SMR	O31611. Positions 4-203.
ModBase	Search...
Protein-protein interaction databases
STRING	224308.BSU11600.
Proteomic databases
PaxDb	O31611.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB13017; CAB13017; BSU11600.
GeneID	939809.
KEGG	bsu:BSU11600.
PATRIC	18974037. VBIBacSub10457_1212.
Organism-specific databases
GenoList	BSU11600.
Phylogenomic databases
eggNOG	COG2357.
HOGENOM	HOG000263233.
KO	K07816.
OMA	RSYHVIV.
ProtClustDB	CLSK887070.
Enzyme and pathway databases
BioCyc	BSUB:BSU11600-MONOMER.
UniPathway	UPA00908; UER00884.
Family and domain databases
InterPro	IPR007685. RelA_SpoT. [Graphical view]
Pfam	PF04607. RelA_SpoT. 1 hit. [Graphical view]
SMART	SM00954. RelA_SpoT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YJBM_BACSU

Accession

Primary (citable) accession number: O31611

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 19, 2010
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program