Reviewed, UniProtKB/Swiss-Prot O31544 (YFJP_BACSU)

Names and origin

Protein names

Recommended name:
    Putative DNA-3-methyladenine glycosylase yfjP
    EC=3.2.2.21

Gene names

Name:	yfjP
Ordered Locus Names:	BSU08010

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2-methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions By similarity.
Catalytic activity	Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.
Sequence similarities	Belongs to the alkylbase DNA glycosidase alkA family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	base-excision repair Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro alkylbase DNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	Putative DNA-3-methyladenine glycosylase yfjP		PRO_0000360864
Sites
	Active site	242	1	Proton acceptor By similarity
	Site	222	1	Determinant for substrate specificity and/or activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	O31544-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 89790D5090FDE589	FASTA	287	33,178
	10 20 30 40 50 60 MWKEKVSVTP PYHFDRVLDR LSLDPLNAVD REAREVRVPI RNQAGDVCIV KVQALGHAGE 70 80 90 100 110 120 PEFLVSGETD QGEMMKEIKR IFQWENHLQH VLDHFSKTSL SAIFEEHAGT PLVLDYSVYN 130 140 150 160 170 180 CMMKCIIHQQ LNLSFAYTLT ERFVHAFGEQ KDGVWCYPKP ETIAELDYQD LRDLQFSMRK 190 200 210 220 230 240 AEYTIDTSRM IAEGTLSLSE LPHMADEDIM KKLIKIRGIG PWTVQNVLMF GLGRPNLFPL 250 260 270 280 ADIGLQNAIK RHFQLDDKPA KDVMLAMSKE WEPYLSYASL YLWRSIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of a 40.6 kb segment in the 73 degrees-76 degrees region of the Bacillus subtilis chromosome containing genes for trehalose metabolism and acetoin utilization." Yamamoto H., Uchiyama S., Sekiguchi J. Microbiology 142:3057-3065(1996) [PubMed: 8969503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / AC327.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D78509 Genomic DNA. Translation: BAA24301.1. AL009126 Genomic DNA. Translation: CAB12630.1.
PIR	G69806.
RefSeq	NP_388682.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	939192.
GenomeReviews	Gene locus BSU08010 in contig AL009126_GR.
KEGG	bsu:BSU08010.
NMPDR	fig|224308.1.peg.801.
Organism-specific databases
SubtiList	BG12912. yfjP. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	O31544.
OMA	WTVQNFL.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU0801-MON.
Family and domain databases
InterPro	IPR000035. Alkylbase_DNA_glycsylse_CS. IPR003265. HhH-GPD_domain. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. [Graphical view]
Pfam	PF00730. HhH-GPD. 1 hit. [Graphical view]
SMART	SM00478. ENDO3c. 1 hit. SM00278. HhH1. 1 hit. [Graphical view]
PROSITE	PS00516. ALKYLBASE_DNA_GLYCOS. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YFJP_BACSU

Accession

Primary (citable) accession number: O31544
Secondary accession number(s): Q79F09

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)