Reviewed, UniProtKB/Swiss-Prot O31528 (RHGT2_BACSU)

Names and origin

Protein names

Recommended name:
    Probable rhamnogalacturonan acetylesterase yesY
      Short name=RGAE
    EC=3.1.1.-

Gene names

Name:	yesY
Ordered Locus Names:	BSU07070

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play role in the degradation of rhamnogalacturonan derived from plant cell walls. Probably has broad substrate specificity and may degrade several types of acetylated substrates. Ref.2
Induction	Up-regulated by growth on type I rhamnogalacturonan. Ref.2
Sequence similarities	Belongs to the 'GDSL' lipolytic enzyme family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	Probable rhamnogalacturonan acetylesterase yesY		PRO_0000364433
Sites
	Active site	11	1	By similarity
	Active site	185	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O31528-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 7FF4EC62C4C473F2	FASTA	217	24,594
	10 20 30 40 50 60 MANHIYLAGD STVQTYGDST NQGGWGQFLG SHLPEHIQVI NRAIGGRSSK TFVEEGRLQA 70 80 90 100 110 120 ILDVIEPDDW LFVQMGHNDA SKNKPERYTE PYTTYKQYLK QYIAGAREKG AHPLLITPVA 130 140 150 160 170 180 RFHYENGVFL NDFPDYCIAM KQTAAEENVQ LIDLMEKSLA FFTEKGEEKV YTYFMISEGI 190 200 210 NDYTHFTKKG ANEMAKLVAK GIKELGLPLT ESIIKER

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[2]	"Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization." Ochiai A., Itoh T., Kawamata A., Hashimoto W., Murata K. Appl. Environ. Microbiol. 73:3803-3813(2007) [PubMed: 17449691] [Abstract] Cited for: FUNCTION IN DEGRADATION OF TYPE I RHAMNOGALACTURONAN, INDUCTION. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL009126 Genomic DNA. Translation: CAB12526.1.
PIR	H69797.
RefSeq	NP_388588.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	938772.
GenomeReviews	Gene locus BSU07070 in contig AL009126_GR.
KEGG	bsu:BSU07070.
NMPDR	fig|224308.1.peg.707.
Organism-specific databases
SubtiList	BG12859. yesY. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	O31528.
OMA	O31528. GHNDASK.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU0707-MON.
Family and domain databases
InterPro	IPR013831. Esterase_SGNH_hydro-type_subgr. IPR001087. Lipase_GDSL. IPR008265. Lipase_GDSL_AS. [Graphical view]
Gene3D	G3DSA:3.40.50.1110. Esterase_SGNH_hydro-type_subgr. 1 hit.
Pfam	PF00657. Lipase_GDSL. 1 hit. [Graphical view]
PROSITE	PS01098. LIPASE_GDSL_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RHGT2_BACSU

Accession

Primary (citable) accession number: O31528

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	January 1, 1998
Last modified:	May 5, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)