O31528 (RHGT2_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable rhamnogalacturonan acetylesterase yesY
Short name=RGAE
EC=3.1.1.-

Gene names

Name:	yesY
Ordered Locus Names:	BSU07070

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play role in the degradation of rhamnogalacturonan derived from plant cell walls. Probably has broad substrate specificity and may degrade several types of acetylated substrates. Ref.2
Induction	Up-regulated by growth on type I rhamnogalacturonan. Ref.2
Sequence similarities	Belongs to the 'GDSL' lipolytic enzyme family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	Probable rhamnogalacturonan acetylesterase yesY		PRO_0000364433
Sites
	Active site	11	1	Nucleophile By similarity
	Active site	178	1	By similarity
	Active site	185	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O31528 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 7FF4EC62C4C473F2	FASTA	217	24,594
	10 20 30 40 50 60 MANHIYLAGD STVQTYGDST NQGGWGQFLG SHLPEHIQVI NRAIGGRSSK TFVEEGRLQA 70 80 90 100 110 120 ILDVIEPDDW LFVQMGHNDA SKNKPERYTE PYTTYKQYLK QYIAGAREKG AHPLLITPVA 130 140 150 160 170 180 RFHYENGVFL NDFPDYCIAM KQTAAEENVQ LIDLMEKSLA FFTEKGEEKV YTYFMISEGI 190 200 210 NDYTHFTKKG ANEMAKLVAK GIKELGLPLT ESIIKER

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[2]	"Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization." Ochiai A., Itoh T., Kawamata A., Hashimoto W., Murata K. Appl. Environ. Microbiol. 73:3803-3813(2007) [PubMed: 17449691] [Abstract] Cited for: FUNCTION IN DEGRADATION OF TYPE I RHAMNOGALACTURONAN, INDUCTION. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL009126 Genomic DNA. Translation: CAB12526.1.
PIR	H69797.
RefSeq	NP_388588.1. NC_000964.3.
3D structure databases
ProteinModelPortal	O31528.
SMR	O31528. Positions 3-215.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000003862; EBBACP00000003862; EBBACG00000003854.
GeneID	938772.
GenomeReviews	Gene locus BSU07070 in contig AL009126_GR.
KEGG	bsu:BSU07070.
NMPDR	fig|224308.1.peg.707.
PATRIC	18973062. VBIBacSub10457_0745.
Organism-specific databases
GenoList	BSU07070.
Phylogenomic databases
GeneTree	EBGT00050000006183.
HOGENOM	HBG400724.
OMA	GHNDASK.
PhylomeDB	O31528.
ProtClustDB	CLSK886830.
Enzyme and pathway databases
BioCyc	BSUB:BSU07070-MONOMER.
Family and domain databases
InterPro	IPR013830. Esterase_SGNH_hydro-type. IPR013831. Esterase_SGNH_hydro-type_subgr. IPR001087. Lipase_GDSL. [Graphical view]
Gene3D	G3DSA:3.40.50.1110. Esterase_SGNH_hydro-type_subgr. 1 hit.
Pfam	PF00657. Lipase_GDSL. 1 hit. [Graphical view]
SUPFAM	SSF52266. Esterase_SGNH_hydro-type. 1 hit.
PROSITE	PS01098. LIPASE_GDSL_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RHGT2_BACSU

Accession

Primary (citable) accession number: O31528

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program