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Protein

Diacylglycerol kinase

Gene

dagK

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthetic pathway. Is more active toward long-chain DAG compared with short-chain DAG. Is not able to phosphorylate substrates other than DAG, such as monoacylglycerol, ceramide, undecaprenol, phosphatidylinositol, or sphingosine.1 Publication

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. This ion appears to have a structural role and is required for catalytic activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40ATPPROSITE-ProRule annotation1
Binding sitei93ATPPROSITE-ProRule annotation1
Metal bindingi213Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi216MagnesiumBy similarity1
Metal bindingi218Magnesium; via carbonyl oxygenBy similarity1
Active sitei273Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 13ATPPROSITE-ProRule annotation5
Nucleotide bindingi66 – 72ATPPROSITE-ProRule annotation7

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • diacylglycerol kinase activity Source: CACAO
  • metal ion binding Source: UniProtKB-KW
  • NAD+ kinase activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU06720-MONOMER

Chemistry databases

SwissLipidsiSLP:000001808

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase (EC:2.7.1.107)
Short name:
DAG kinase
Short name:
DAGK
Gene namesi
Name:dagK
Synonyms:dgkB, yerQ
Ordered Locus Names:BSU06720
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are not viable. They exhibit accumulation of diacylglycerol, disappearance of phosphatidylglycerol, and the cessation of lipoteichoic acid formation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003864821 – 303Diacylglycerol kinaseAdd BLAST303

Proteomic databases

PaxDbiO31502
PRIDEiO31502

Interactioni

Protein-protein interaction databases

IntActiO31502, 2 interactors
STRINGi224308.Bsubs1_010100003793

Structurei

3D structure databases

ProteinModelPortaliO31502
SMRiO31502
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 132DAGKcPROSITE-ProRule annotationAdd BLAST132

Sequence similaritiesi

Belongs to the diacylglycerol/lipid kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105CBD Bacteria
COG1597 LUCA
HOGENOMiHOG000261395
InParanoidiO31502
KOiK07029
OMAiEHINDPH
PhylomeDBiO31502

Family and domain databases

Gene3Di3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR005218 Diacylglycerol/lipid_kinase
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf
PfamiView protein in Pfam
PF00781 DAGK_cat, 1 hit
SMARTiView protein in SMART
SM00046 DAGKc, 1 hit
SUPFAMiSSF111331 SSF111331, 1 hit
TIGRFAMsiTIGR00147 TIGR00147, 1 hit
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit

Sequencei

Sequence statusi: Complete.

O31502-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRARIIYNP TSGREIFKKH LAQVLQKFEQ AGYETSTHAT TCAGDATHAA
60 70 80 90 100
KEAALREFDL IIAAGGDGTI NEVVNGLAPL DNRPTLGVIP VGTTNDFARA
110 120 130 140 150
LGIPREDILK AADTVINGVA RPIDIGQVNG QYFINIAGGG RLTELTYDVP
160 170 180 190 200
SKLKTMLGQL AYYLKGMEML PSLRPTEVEI EYDGKLFQGE IMLFLVTLTN
210 220 230 240 250
SVGGFEKLAP DSSLNDGMFD LMILKKANLA EFIRVATMAL RGEHINDQHI
260 270 280 290 300
IYTKANRVKV NVSEKMQLNL DGEYGGMLPG EFVNLYRHIH VVMPKEKAEQ

LDD
Length:303
Mass (Da):33,337
Last modified:January 1, 1998 - v1
Checksum:i533F3658A41A5998
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB12492.1
PIRiF69795
RefSeqiNP_388554.1, NC_000964.3
WP_003233894.1, NZ_JNCM01000032.1

Genome annotation databases

EnsemblBacteriaiCAB12492; CAB12492; BSU06720
GeneIDi936061
KEGGibsu:BSU06720
PATRICifig|224308.179.peg.730

Similar proteinsi

Entry informationi

Entry nameiDAGK_BACSU
AccessioniPrimary (citable) accession number: O31502
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

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