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Reviewed, UniProtKB/Swiss-Prot O31498 (DNLJ_BACSU)

Last modified November 3, 2009. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    DNA ligase
    EC=6.5.1.2
Alternative name(s):
    Polydeoxyribonucleotide synthase [NAD+]
Gene names
Name: ligA
Synonyms: lig, yerG
Ordered Locus Names: BSU06620
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length668 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity.

Catalytic activity

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588

Cofactor

Magnesium or manganese By similarity.

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Contains 1 BRCT domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 668668DNA ligase HAMAP MF_01588
PRO_0000161738

Regions

Domain590 – 66879BRCT
Nucleotide binding34 – 385NAD By similarity
Nucleotide binding83 – 842NAD By similarity

Sites

Active site1151N6-AMP-lysine intermediate By similarity
Metal binding4041Zinc By similarity
Metal binding4071Zinc By similarity
Metal binding4221Zinc By similarity
Metal binding4271Zinc By similarity
Binding site1131NAD By similarity
Binding site1361NAD By similarity
Binding site1701NAD By similarity
Binding site2861NAD By similarity
Binding site3101NAD By similarity

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Sequences

Sequence LengthMass (Da)Tools
O31498-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: AC86449BC975DB3F

FASTA66874,876
        10         20         30         40         50         60 
MDKETAKQRA EELRRTINKY SYEYYTLDEP SVPDAEYDRL MQELIAIEEE HPDLRTPDSP 

        70         80         90        100        110        120 
TQRVGGAVLE AFQKVTHGTP MLSLGNAFNA DDLRDFDRRV RQSVGDDVAY NVELKIDGLA 

       130        140        150        160        170        180 
VSLRYEDGYF VRGATRGDGT TGEDITENLK TIRNIPLKMN RELSIEVRGE AYMPKRSFEA 

       190        200        210        220        230        240 
LNEERIKNEE EPFANPRNAA AGSLRQLDPK IAAKRNLDIF VYSIAELDEM GVETQSQGLD 

       250        260        270        280        290        300 
FLDELGFKTN QERKKCGSIE EVITLIDELQ AKRADLPYEI DGIVIKVDSL DQQEELGFTA 

       310        320        330        340        350        360 
KSPRWAIAYK FPAEEVVTKL LDIELNVGRT GVITPTAILE PVKVAGTTVS RASLHNEDLI 

       370        380        390        400        410        420 
KEKDIRILDK VVVKKAGDII PEVVNVLVDQ RTGEEKEFSM PTECPECGSE LVRIEGEVAL 

       430        440        450        460        470        480 
RCINPECPAQ IREGLIHFVS RNAMNIDGLG ERVITQLFEE NLVRNVADLY KLTKERVIQL 

       490        500        510        520        530        540 
ERMGEKSTEN LISSIQKSKE NSLERLLFGL GIRFIGSKAA KTLAMHFESL ENLKKASKEE 

       550        560        570        580        590        600 
LLAVDEIGEK MADAVITYFH KEEMLELLNE LQELGVNTLY KGPKKVKAED SDSYFAGKTI 

       610        620        630        640        650        660 
VLTGKLEELS RNEAKAQIEA LGGKLTGSVS KNTDLVIAGE AAGSKLTKAQ ELNIEVWNEE 


QLMGELKK

« Hide

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References

« Hide 'large scale' references
[1]"PcrA is an essential DNA helicase of Bacillus subtilis fulfilling functions both in repair and rolling-circle replication."
Petit M.-A., Dervyn E., Rose M., Entian K.-D., McGovern S., Ehrlich S.D., Bruand C.
Mol. Microbiol. 29:261-273(1998) [PubMed: 9701819] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.

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Cross-references

Sequence databases

Y15254 Genomic DNA. No translation available.
AL009126 Genomic DNA. Translation: CAB12482.1.
PIRF69794.
RefSeqNP_388544.1.

3D structure databases

HSSPHSSP built from PDB template 1B04 based on UniProtKB O87703.
ModBaseSearch...

Genome annotation databases

GeneID936056.
GenomeReviewsGene locus BSU06620 in contig AL009126_GR.
KEGGbsu:BSU06620.
NMPDRfig|224308.1.peg.663.

Organism-specific databases

SubtiListBG12835. ligA. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMO31498.
OMAYKFPAQE.

Enzyme and pathway databases

BioCycBSUB224308:BSU0663-MON.
BRENDA6.5.1.2. 150.

Family and domain databases

HAMAPMF_01588.
[Tree]
InterProIPR001357. BRCT.
IPR018239. DNA_ligase_AS.
IPR004150. DNA_ligase_OB.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR004149. Znf_DNAligase_C4.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PfamPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
PF00633. HHH. 1 hit.
[Graphical view]
PIRSFPIRSF001604. LigA. 1 hit.
ProDomPD003944. DNAligase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00292. BRCT. 1 hit.
SM00278. HhH1. 3 hits.
SM00532. LIGANc. 1 hit.
[Graphical view]
TIGRFAMsTIGR00575. dnlj. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. 1 hit.
PS01056. DNA_LIGASE_N2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameDNLJ_BACSU
AccessionPrimary (citable) accession number: O31498
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 3, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents