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Protein

Glutaminase 1

Gene

glsA1

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.1 Publication

Kineticsi

  1. KM=27.6 mM for glutamine1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei74Substrate1
    Binding sitei126Substrate1
    Binding sitei170Substrate1
    Binding sitei177Substrate1
    Binding sitei201Substrate1
    Binding sitei253Substrate1
    Binding sitei271Substrate; via amide nitrogen1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciBSUB:BSU02430-MONOMER.
    BRENDAi3.5.1.2. 658.
    4.3.3.6. 658.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutaminase 1 (EC:3.5.1.2)
    Gene namesi
    Name:glsA1
    Synonyms:glsA, ybgJ
    Ordered Locus Names:BSU02430
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001105941 – 327Glutaminase 1Add BLAST327

    Proteomic databases

    PaxDbiO31465.
    PRIDEiO31465.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100001348.

    Structurei

    Secondary structure

    1327
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi14 – 25Combined sources12
    Helixi26 – 31Combined sources6
    Helixi39 – 42Combined sources4
    Beta strandi50 – 54Combined sources5
    Beta strandi60 – 65Combined sources6
    Helixi73 – 75Combined sources3
    Helixi76 – 88Combined sources13
    Helixi90 – 94Combined sources5
    Helixi110 – 114Combined sources5
    Beta strandi123 – 125Combined sources3
    Helixi126 – 135Combined sources10
    Beta strandi136 – 140Combined sources5
    Helixi141 – 156Combined sources16
    Helixi164 – 173Combined sources10
    Helixi175 – 186Combined sources12
    Helixi194 – 205Combined sources12
    Beta strandi207 – 209Combined sources3
    Helixi211 – 222Combined sources12
    Turni223 – 225Combined sources3
    Turni228 – 231Combined sources4
    Helixi237 – 250Combined sources14
    Helixi253 – 255Combined sources3
    Helixi256 – 262Combined sources7
    Beta strandi267 – 269Combined sources3
    Beta strandi273 – 279Combined sources7
    Turni281 – 284Combined sources4
    Turni288 – 291Combined sources4
    Beta strandi293 – 298Combined sources6
    Beta strandi306 – 308Combined sources3
    Helixi309 – 322Combined sources14

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1MKIX-ray2.00A/B1-327[»]
    2OSUX-ray2.29A/B1-327[»]
    3AGFX-ray2.60A/B1-327[»]
    3BRMX-ray2.29A/B1-327[»]
    ProteinModelPortaliO31465.
    SMRiO31465.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO31465.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutaminase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105CSV. Bacteria.
    COG2066. LUCA.
    HOGENOMiHOG000216890.
    InParanoidiO31465.
    KOiK01425.
    OMAiPMNPMVN.
    PhylomeDBiO31465.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    HAMAPiMF_00313. Glutaminase. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view]
    PANTHERiPTHR12544. PTHR12544. 1 hit.
    PfamiPF04960. Glutaminase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR03814. Gln_ase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O31465-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKELIKEHQK DINPALQLHD WVEYYRPFAA NGQSANYIPA LGKVNDSQLG
    60 70 80 90 100
    ICVLEPDGTM IHAGDWNVSF TMQSISKVIS FIAACMSRGI PYVLDRVDVE
    110 120 130 140 150
    PTGDAFNSII RLEINKPGKP FNPMINAGAL TIASILPGES AYEKLEFLYS
    160 170 180 190 200
    VMETLIGKRP RIHEEVFRSE WETAHRNRAL AYYLKETNFL EAEVEETLEV
    210 220 230 240 250
    YLKQCAMEST TEDIALIGLI LAHDGYHPIR HEQVIPKDVA KLAKALMLTC
    260 270 280 290 300
    GMYNASGKYA AFVGVPAKSG VSGGIMALVP PSARREQPFQ SGCGIGIYGP
    310 320
    AIDEYGNSLT GGMLLKHMAQ EWELSIF
    Length:327
    Mass (Da):36,187
    Last modified:January 1, 1998 - v1
    Checksum:iBE3A1C2366460287
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB006424 Genomic DNA. Translation: BAA33141.1.
    AL009126 Genomic DNA. Translation: CAB12037.1.
    PIRiD69751.
    RefSeqiNP_388125.1. NC_000964.3.
    WP_003234829.1. NZ_JNCM01000030.1.

    Genome annotation databases

    EnsemblBacteriaiCAB12037; CAB12037; BSU02430.
    GeneIDi938416.
    KEGGibsu:BSU02430.
    PATRICi18972037. VBIBacSub10457_0247.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB006424 Genomic DNA. Translation: BAA33141.1.
    AL009126 Genomic DNA. Translation: CAB12037.1.
    PIRiD69751.
    RefSeqiNP_388125.1. NC_000964.3.
    WP_003234829.1. NZ_JNCM01000030.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1MKIX-ray2.00A/B1-327[»]
    2OSUX-ray2.29A/B1-327[»]
    3AGFX-ray2.60A/B1-327[»]
    3BRMX-ray2.29A/B1-327[»]
    ProteinModelPortaliO31465.
    SMRiO31465.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100001348.

    Proteomic databases

    PaxDbiO31465.
    PRIDEiO31465.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB12037; CAB12037; BSU02430.
    GeneIDi938416.
    KEGGibsu:BSU02430.
    PATRICi18972037. VBIBacSub10457_0247.

    Phylogenomic databases

    eggNOGiENOG4105CSV. Bacteria.
    COG2066. LUCA.
    HOGENOMiHOG000216890.
    InParanoidiO31465.
    KOiK01425.
    OMAiPMNPMVN.
    PhylomeDBiO31465.

    Enzyme and pathway databases

    BioCyciBSUB:BSU02430-MONOMER.
    BRENDAi3.5.1.2. 658.
    4.3.3.6. 658.

    Miscellaneous databases

    EvolutionaryTraceiO31465.
    PROiO31465.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    HAMAPiMF_00313. Glutaminase. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view]
    PANTHERiPTHR12544. PTHR12544. 1 hit.
    PfamiPF04960. Glutaminase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGLSA1_BACSU
    AccessioniPrimary (citable) accession number: O31465
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: January 1, 1998
    Last modified: November 2, 2016
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.