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O31422 (SKFA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sporulation-killing factor SkfA
Gene names
Name:skfA
Synonyms:ybcO
Ordered Locus Names:BSU01910
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length55 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation. Can also inhibit growth of other bacteria at high concentrations. Has a role in protecting the secreted lipase LipA against proteolysis, either by modulating its folding or by acting as a protease inhibitor. Ref.4 Ref.5

Subcellular location

Secreted. Note: Secreted by the ABC transporter SkfEF Probable.

Induction

By Spo0A and PhoP, during nutrient starvation, especially phosphate starvation. Repressed by AbrB during normal growth when nutrients are plentiful, in association with the transcriptional repressor Abh. Ref.7

Ontologies

Keywords
   Cellular componentSecreted
   Molecular functionAntibiotic
Antimicrobial
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response to bacterium

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5555Sporulation-killing factor SkfA
PRO_0000049459

Sequences

Sequence LengthMass (Da)Tools
O31422 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: F169E627ADBEBB55

FASTA555,934
        10         20         30         40         50 
MKRNQKEWES VSKKGLMKPG GTSIVKAAGC MGCWASKSIA MTRVCALPHP AMRAI 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of the 70kb region between 17 and 23 degree of the Bacillus subtilis chromosome."
Haga K., Liu H., Yasumoto K., Takahashi H., Yoshikawa H.
Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"A 3.1-kb genomic fragment of Bacillus subtilis encodes the protein inhibiting growth of Xanthomonas oryzae pv. oryzae."
Lin D., Qu L.-J., Gu H., Chen Z.
J. Appl. Microbiol. 91:1044-1050(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INHIBITION OF XANTHOMONAS GROWTH.
[4]"Cannibalism by sporulating bacteria."
Gonzalez-Pastor J.E., Hobbs E.C., Losick R.
Science 301:510-513(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: 168 / PY79.
[5]"Genes involved in SkfA killing factor production protect a Bacillus subtilis lipase against proteolysis."
Westers H., Braun P.G., Westers L., Antelmann H., Hecker M., Jongbloed J.D.H., Yoshikawa H., Tanaka T., van Dijl J.M., Quax W.J.
Appl. Environ. Microbiol. 71:1899-1908(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ROLE IN LIPASE PRODUCTION.
Strain: 168.
[6]"High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis."
Fujita M., Gonzalez-Pastor J.E., Losick R.
J. Bacteriol. 187:1357-1368(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: REPRESSION BY ABRB.
[7]"Phosphate starvation induces the sporulation killing factor of Bacillus subtilis."
Allenby N.E.E., Watts C.A., Homuth G., Pragai Z., Wipat A., Ward A.C., Harwood C.R.
J. Bacteriol. 188:5299-5303(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
Strain: 168.
[8]"Abh and AbrB control of Bacillus subtilis antimicrobial gene expression."
Strauch M.A., Bobay B.G., Cavanagh J., Yao F., Wilson A., Le Breton Y.
J. Bacteriol. 189:7720-7732(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: REPRESSION BY ABRB AND ABH.

Web resources

Protein Spotlight

I'll have you for supper - Issue 90 of January 2008

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB006424 Genomic DNA. Translation: BAA33086.1.
AL009126 Genomic DNA. Translation: CAB11984.1.
PIRC69746.
RefSeqNP_388072.1. NC_000964.3.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU01910.

Proteomic databases

PaxDbO31422.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB11984; CAB11984; BSU01910.
GeneID938506.
KEGGbsu:BSU01910.
PATRIC38335271. VBIBacSub146942_0192.

Organism-specific databases

GenoListBSU01910. [Micado]

Phylogenomic databases

OMAPIETANE.

Enzyme and pathway databases

BioCycBSUB:BSU01910-MONOMER.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameSKFA_BACSU
AccessionPrimary (citable) accession number: O31422
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList