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Protein

Sporulation-killing factor SkfA

Gene

skfA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation. Can also inhibit growth of other bacteria at high concentrations. Has a role in protecting the secreted lipase LipA against proteolysis, either by modulating its folding or by acting as a protease inhibitor.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial

Enzyme and pathway databases

BioCyciBSUB:BSU01910-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sporulation-killing factor SkfA
Gene namesi
Name:skfA
Synonyms:ybcO
Ordered Locus Names:BSU01910
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU01910. [Micado]

Subcellular locationi

  • Secreted

  • Note: Secreted by the ABC transporter SkfEF.Curated

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5555Sporulation-killing factor SkfAPRO_0000049459Add
BLAST

Proteomic databases

PaxDbiO31422.

Expressioni

Inductioni

By Spo0A and PhoP, during nutrient starvation, especially phosphate starvation. Repressed by AbrB during normal growth when nutrients are plentiful, in association with the transcriptional repressor Abh.3 Publications

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100001078.

Family & Domainsi

Family and domain databases

InterProiIPR030919. RiPP_SkfA.
[Graphical view]
TIGRFAMsiTIGR04404. RiPP_SkfA. 1 hit.

Sequencei

Sequence statusi: Complete.

O31422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRNQKEWES VSKKGLMKPG GTSIVKAAGC MGCWASKSIA MTRVCALPHP

AMRAI
Length:55
Mass (Da):5,934
Last modified:January 1, 1998 - v1
Checksum:iF169E627ADBEBB55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006424 Genomic DNA. Translation: BAA33086.1.
AL009126 Genomic DNA. Translation: CAB11984.1.
PIRiC69746.
RefSeqiNP_388072.1. NC_000964.3.
WP_009966416.1. NZ_JNCM01000030.1.

Genome annotation databases

EnsemblBacteriaiCAB11984; CAB11984; BSU01910.
GeneIDi938506.
KEGGibsu:BSU01910.
PATRICi38335271. VBIBacSub146942_0192.

Cross-referencesi

Web resourcesi

Protein Spotlight

I'll have you for supper - Issue 90 of January 2008

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006424 Genomic DNA. Translation: BAA33086.1.
AL009126 Genomic DNA. Translation: CAB11984.1.
PIRiC69746.
RefSeqiNP_388072.1. NC_000964.3.
WP_009966416.1. NZ_JNCM01000030.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100001078.

Proteomic databases

PaxDbiO31422.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11984; CAB11984; BSU01910.
GeneIDi938506.
KEGGibsu:BSU01910.
PATRICi38335271. VBIBacSub146942_0192.

Organism-specific databases

GenoListiBSU01910. [Micado]

Enzyme and pathway databases

BioCyciBSUB:BSU01910-MONOMER.

Family and domain databases

InterProiIPR030919. RiPP_SkfA.
[Graphical view]
TIGRFAMsiTIGR04404. RiPP_SkfA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the 70kb region between 17 and 23 degree of the Bacillus subtilis chromosome."
    Haga K., Liu H., Yasumoto K., Takahashi H., Yoshikawa H.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "A 3.1-kb genomic fragment of Bacillus subtilis encodes the protein inhibiting growth of Xanthomonas oryzae pv. oryzae."
    Lin D., Qu L.-J., Gu H., Chen Z.
    J. Appl. Microbiol. 91:1044-1050(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INHIBITION OF XANTHOMONAS GROWTH.
  4. Cited for: FUNCTION.
    Strain: 168 / PY79.
  5. "Genes involved in SkfA killing factor production protect a Bacillus subtilis lipase against proteolysis."
    Westers H., Braun P.G., Westers L., Antelmann H., Hecker M., Jongbloed J.D.H., Yoshikawa H., Tanaka T., van Dijl J.M., Quax W.J.
    Appl. Environ. Microbiol. 71:1899-1908(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN LIPASE PRODUCTION.
    Strain: 168.
  6. "High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis."
    Fujita M., Gonzalez-Pastor J.E., Losick R.
    J. Bacteriol. 187:1357-1368(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REPRESSION BY ABRB.
  7. "Phosphate starvation induces the sporulation killing factor of Bacillus subtilis."
    Allenby N.E.E., Watts C.A., Homuth G., Pragai Z., Wipat A., Ward A.C., Harwood C.R.
    J. Bacteriol. 188:5299-5303(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: 168.
  8. "Abh and AbrB control of Bacillus subtilis antimicrobial gene expression."
    Strauch M.A., Bobay B.G., Cavanagh J., Yao F., Wilson A., Le Breton Y.
    J. Bacteriol. 189:7720-7732(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: REPRESSION BY ABRB AND ABH.

Entry informationi

Entry nameiSKFA_BACSU
AccessioniPrimary (citable) accession number: O31422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: January 1, 1998
Last modified: June 24, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.