Reviewed, UniProtKB/Swiss-Prot O31219 (DHAS_LEGPN)

Names and origin

Protein names

Recommended name:
    Aspartate-semialdehyde dehydrogenase
      Short name=ASA dehydrogenase
      Short name=ASADH
    EC=1.2.1.11

Gene names

Name:

asd

Organism

Legionella pneumophila

Taxonomic identifier

446 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 2/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the aspartate-semialdehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro aspartate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Aspartate-semialdehyde dehydrogenase		PRO_0000141378
Sites
	Active site	132	1	Acyl-thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O31219-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 33D992101D707943	FASTA	347	37,714
	10 20 30 40 50 60 MSRHLNVAIV GATGAVGETF LTVLEERNFP IKSLYPLASS RSVGKTVTFR DQELDVLDLA 70 80 90 100 110 120 EFDFSKVDLA LFSAGGAVSK EYAPKAVAAG CVVVDNTSCF RYEDDIPLVV PGSESSSNRD 130 140 150 160 170 180 YTKRGIIANP NCSTIQMVVA LKPIYDAVGI SRINVATYQS VSGTGKKAIS ELVAQVGDLL 190 200 210 220 230 240 NGRPANVQVY PQQIAFNALP HIDQFEDNGY TREEMKMVWE TRKIMEDDSI MVNPTAVRVP 250 260 270 280 290 300 VIYGHSEAVH LELKKPLTAD DARALLAKAP GVTVVDNLSK ASYPTAIKNA VGHDDVFVGR 310 320 330 340 IRQDISHPCG LNLWIVADNI RKGAATNAVQ IAEILQREFL LKLSLPQ

References

[1]	"Identification of the aspartate-beta-semialdehyde dehydrogenase gene of Legionella pneumophila and characterization of a null mutant." Harb O.S., Abu Kwaik Y. Infect. Immun. 66:1898-1903(1998) [PubMed: 9573067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AA100 / Serogroup 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF034213 Genomic DNA. Translation: AAC46292.1.
3D structure databases
HSSP	HSSP built from PDB template 1BRM based on UniProtKB P00353.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.2.1.11. 1454.
Family and domain databases
InterPro	IPR000319. Asp-semialdehyde_DH_CS. IPR012080. Asp_semialdehyde_DH. IPR005986. Asp_semialdehyde_DH_bac. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_C. [Graphical view]
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
PIRSF	PIRSF000148. ASA_dh. 1 hit.
TIGRFAMs	TIGR01296. asd_B. 1 hit.
PROSITE	PS01103. ASD. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAS_LEGPN

Accession

Primary (citable) accession number: O31219

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)