Reviewed, UniProtKB/Swiss-Prot O31214 (UCRI_CHRVI)

Names and origin

Protein names

Recommended name:
    Ubiquinol-cytochrome c reductase iron-sulfur subunit
    EC=1.10.2.2
Alternative name(s):
    Rieske iron-sulfur protein
      Short name=RISP

Gene names

Name:

petA

Organism

Chromatium vinosum (Allochromatium vinosum)

Taxonomic identifier

1049 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Chromatiaceae › Allochromatium

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.
Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Ubiquinol-cytochrome c reductase iron-sulfur subunit		PRO_0000127760
Regions
	Transmembrane	24 – 44	21	Potential
	Domain	100 – 199	100	Rieske
Sites
	Metal binding	134	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	136	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	162	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	165	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	139 ↔ 164		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O31214-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: C6886EDF25E981CD	FASTA	207	22,125
	10 20 30 40 50 60 MLASAGGYWP MSAQGVNKMR RRVLVAATSV VGAVGAGYAL VPFVASMNPS ARARAAGAPV 70 80 90 100 110 120 EADISKLEPG ALLRVKWRGM PVWVVHRSSE MLAALSSNDP KLVDPTSEVP QQPDYCKNPT 130 140 150 160 170 180 RSIKPEYLVA IGICTHLGCS PTYRPEFGPD DLGSGWKGGF HCPCHGSRFD LAARVFKNVP 190 200 APTNLVIPKH VYLNDTTILI GEDRGSA

References

[1]

"The pet operon, encoding the prosthetic group-containing subunits of the cytochrome bc1 complex, of the purple sulfur bacterium Chromatium vinosum." Chen Y.L., Dincturk H.B., Qin H., Knaff D.B. Photosyn. Res. 57:149-158(1998) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 17899 / DSM 180 / D.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF034104 Genomic DNA. Translation: AAB86973.1.
3D structure databases
HSSP	HSSP built from PDB template 1RIE based on UniProtKB P13272.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.10.2.2. 2799.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. IPR019470. Ubiq_cytC_Rdtase_Fe-S_su_TAT. IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF10399. UCR_Fe-S_N. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
TIGRFAMs	TIGR01416. Rieske_proteo. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_CHRVI

Accession

Primary (citable) accession number: O31214

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	October 13, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)