Reviewed, UniProtKB/Swiss-Prot O31112 (FWDC_METMP)

Names and origin

Protein names

Recommended name:
    Tungsten-containing formylmethanofuran dehydrogenase 2 subunit C
    EC=1.2.99.5
Alternative name(s):
    Tungsten-containing formylmethanofuran dehydrogenase II subunit C

Gene names

Name:	fwdC
Ordered Locus Names:	MMP1249

Organism

Methanococcus maripaludis [Complete proteome] [HAMAP]

Taxonomic identifier

39152 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of CO2 and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). This enzyme is oxygen-labile By similarity.
Catalytic activity	Formylmethanofuran + H2O + acceptor = CO2 + methanofuran + reduced acceptor.
Cofactor	Tungsten By similarity.
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methenyl-H(4)MPT from CO(2): step 1/3.
Subunit structure	This enzyme is composed of seven subunits fwdA (65 kDa), fwdB (53 kDa), fwdC (31 kDa), fwdD (15 kDa), fwdE, fwdF and fwdG By similarity.
Sequence similarities	Belongs to the fwdC/fmdC family.

Ontologies

Keywords
Biological process	Methanogenesis
Domain	Repeat
Ligand	Tungsten
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	formylmethanofuran dehydrogenase activity Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Tungsten-containing formylmethanofuran dehydrogenase 2 subunit C		PRO_0000144195
Regions
	Repeat	77 – 89	13	1
	Repeat	96 – 108	13	2
	Repeat	115 – 127	13	3
	Repeat	141 – 153	13	4
	Repeat	160 – 172	13	5
	Repeat	179 – 191	13	6
	Repeat	198 – 210	13	7
	Region	77 – 210	134	7 X 13 AA repeats of [GW]-X-X-[MLP]-X-X-G-X-[IL]-X-[IV]-X-G
Experimental info
	Sequence conflict	22	1	L → I in AAB82591. Ref.1
	Sequence conflict	33	1	E → K in AAB82591. Ref.1
	Sequence conflict	60 – 62	3	DVA → PVS in AAB82591. Ref.1
	Sequence conflict	95	1	I → V in AAB82591. Ref.1
	Sequence conflict	154	1	N → T in AAB82591. Ref.1
	Sequence conflict	182	1	Q → P in AAB82591. Ref.1
	Sequence conflict	212	1	I → T in AAB82591. Ref.1
	Sequence conflict	233	1	S → F in AAB82591. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O31112-1 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: F87AA3DE97D34DC0	FASTA	272	29,187
	10 20 30 40 50 60 MNELILNLKG DVSVPVEMDK ILPEKIQEMS LEEISGIELI QGNKTAKVSE IFDVELKESD 70 80 90 100 110 120 VAKVTINNCC KKVKRIGEKM TSGEIVVNGD AGMYIGVEMK GGKITVNGDA ESWVGQNLKG 130 140 150 160 170 180 GEIIINGNAE NYVGSAYRGD WRGMSGGKIT ITGNAGSELG EYLKGGTIVI KGNTKIMPGI 190 200 210 220 230 240 HQNGGMIIIE GDIEGRAGGE MMKGAIVVYG KILEPLPSFK FEGIVEDPLV KLSKKDAGTQ 250 260 270 LKGTFIKFSG DYVNTKPKGQ LYAAIENNKN LI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Yu J.-P., Whitman W.B. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JJ.
[2]	"Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis." Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J., Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M., Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A., Moore B.C. , Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D., Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W., Olson M.V., Leigh J.A. J. Bacteriol. 186:6956-6969(2004) [PubMed: 15466049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S2 / LL.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF029842 Genomic DNA. Translation: AAB82591.1. BX950229 Genomic DNA. Translation: CAF30805.1.
RefSeq	NP_988369.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2762780.
GenomeReviews	Gene locus MMP1249 in contig BX950229_GR.
KEGG	mmp:MMP1249.
NMPDR	fig|267377.1.peg.1249.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O31112.
OMA	O31112. YVGSAYR.
Enzyme and pathway databases
BioCyc	MMAR267377:MMP1249-MON.
BRENDA	1.2.99.5. 20956.
Family and domain databases
InterPro	IPR017550. Formylmethanofuran_DH_suC. IPR002489. Glu_synthase_C. [Graphical view]
Gene3D	G3DSA:2.160.20.60. Glu_synthase_C. 1 hit.
Pfam	PF01493. GXGXG. 1 hit. [Graphical view]
TIGRFAMs	TIGR03122. one_C_dehyd_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FWDC_METMP

Accession

Primary (citable) accession number: O31112

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	April 13, 2004
Last modified:	June 16, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)