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Protein

Solvent-resistant pump membrane transporter SrpB

Gene

srpB

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

The inner membrane transporter component of an organic solvent efflux pump. Involved in export of a number of low log POW compounds including hexane (log POW 3.5), toluene (log POW 2.5) and dimethylphthalate (log POW 2.3). The solvent resistance phenotype has been postulated to depend on the operon expression level.

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Solvent-resistant pump membrane transporter SrpB
Gene namesi
Name:srpB
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 3021HelicalSequence analysisAdd
BLAST
Transmembranei339 – 35921HelicalSequence analysisAdd
BLAST
Transmembranei366 – 38621HelicalSequence analysisAdd
BLAST
Transmembranei392 – 41221HelicalSequence analysisAdd
BLAST
Transmembranei440 – 46021HelicalSequence analysisAdd
BLAST
Transmembranei470 – 49021HelicalSequence analysisAdd
BLAST
Transmembranei542 – 56221HelicalSequence analysisAdd
BLAST
Transmembranei871 – 89121HelicalSequence analysisAdd
BLAST
Transmembranei895 – 91521HelicalSequence analysisAdd
BLAST
Transmembranei927 – 94721HelicalSequence analysisAdd
BLAST
Transmembranei973 – 99321HelicalSequence analysisAdd
BLAST
Transmembranei1008 – 102821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10491049Solvent-resistant pump membrane transporter SrpBPRO_0000161855Add
BLAST

Expressioni

Inductioni

Low constitutive expression; the srpABC operon is further induced up to 17-fold by organic solvents (e.g. toluene and aliphatic solvents and alcohols) but not by antibiotics, heavy metals, pH, temperature, high NaCl or 60 mM acetic acid.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO31100.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.30.2090.10. 2 hits.
InterProiIPR027463. AcrB_DN_DC_subdom.
IPR001036. Acrflvin-R.
IPR004764. HAE1.
[Graphical view]
PfamiPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSiPR00702. ACRIFLAVINRP.
SUPFAMiSSF82714. SSF82714. 2 hits.
TIGRFAMsiTIGR00915. 2A0602. 1 hit.

Sequencei

Sequence statusi: Complete.

O31100-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRFFIDRPI FAWVLAIVAM LAGALSLAKM PISQYPNIAA PAVSIQVSYP
60 70 80 90 100
GASAQTVQDT VVQVIEQQLS GLDGFRYMSA ESASDGSMTI IVTFEQGTDP
110 120 130 140 150
DIAQVQVQNK LQLATPRLPE EVQRQGLRVV KYQMNFFLVM SLVDRSGKLD
160 170 180 190 200
NFDLGNLIAS QLQDPISRIP GVGDFQLFGS PYAMRIWLDP GKLNSYQLTP
210 220 230 240 250
TDVASAIREQ NVQVSSGQLG GLPTRSGVQL NATVLGKTRM TTPSQFDEIL
260 270 280 290 300
VKVNPDGSQV RVKDVGRAEL GADSFAISAQ YKDSPTASLA LRLSTGGNLL
310 320 330 340 350
ETVDAVKKLM EQQKAYLPDG VEVIYPYDTT PVVEASIESV VHTIFEAVVL
360 370 380 390 400
VFLVMYLFLQ SFRATLIPTL AVPVVLLATF ALLPYFGLNI NVLTMYAMVL
410 420 430 440 450
AIGLLVDDAI VVVENVERLM HDEGLSPLEA TRKSMDQISG ALVGIGMVLS
460 470 480 490 500
AVFVPMAFFG GSAGIIYQQF AITIVVCMGL SILVALVFTP ALCVTILKAP
510 520 530 540 550
EGNSHHERKG FFGWFNRIFD RGTRRFERGV GAMLKGRGRY LLAFLLITGG
560 570 580 590 600
TGYLFTQIPK AFLPNEDQGL MMIEVRTPAN ASAERTEGVL QEVRDYLAND
610 620 630 640 650
EGALVEHFMT VNGFNFAGRG QNSGLVLITF KDWKERHGAG QDVFSIAQRA
660 670 680 690 700
NQHFAKIKDA SVMAFVPPAI LEMGNAMGFN LYLQDNLGLG HEALMAARNQ
710 720 730 740 750
FLQLASQNPK LQAVRPNGKD DEPQFQVNID DEKARALQVS IASINETMSA
760 770 780 790 800
AWGSMYVNDF IDRGRVKRVY VQGEDISRIS PEDFDKWYVR NSLGQMVPFS
810 820 830 840 850
AFATGEWVNG SPKLERYGGI SSLNILGEPA PGYSTGDAMI AIAEIMQQLP
860 870 880 890 900
AGIGLSYTGL SYEEIQTGDQ APLLYALTVL IVFLCLAALY ESWSVPVSVI
910 920 930 940 950
MVVPLGILGA VLATLWRDLT ADVYFQVGLM TTVGLSAKNA ILIVEFAKEL
960 970 980 990 1000
YEKEGYPIVK AAIEAAKLRL RPILMTSLAF TFGVLPMAIA SGAGAGSQHS
1010 1020 1030 1040
IATGVVGGMI TATVLAVFFV PLFYVVVVKL FEGLMKRKPN AVKEVTHEV
Length:1,049
Mass (Da):114,110
Last modified:January 1, 1998 - v1
Checksum:iA5A0782BE2BBCD81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029405 Genomic DNA. Translation: AAD12176.1.
RefSeqiWP_014003970.1. NZ_LKKS01000033.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029405 Genomic DNA. Translation: AAD12176.1.
RefSeqiWP_014003970.1. NZ_LKKS01000033.1.

3D structure databases

ProteinModelPortaliO31100.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.2090.10. 2 hits.
InterProiIPR027463. AcrB_DN_DC_subdom.
IPR001036. Acrflvin-R.
IPR004764. HAE1.
[Graphical view]
PfamiPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSiPR00702. ACRIFLAVINRP.
SUPFAMiSSF82714. SSF82714. 2 hits.
TIGRFAMsiTIGR00915. 2A0602. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Identification and molecular characterization of an efflux pump involved in Pseudomonas putida S12 solvent tolerance."
    Kieboom J., Dennis J.J., de Bont J.A.M., Zylstra G.J.
    J. Biol. Chem. 273:85-91(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], EFFLUX PUMP SUBSTRATES.
    Strain: ATCC 700801 / S12.
  2. "Active efflux of organic solvents by Pseudomonas putida S12 is induced by solvents."
    Kieboom J., Dennis J.J., Zylstra G.J., de Bont J.A.M.
    J. Bacteriol. 180:6769-6772(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: ATCC 700801 / S12.
  3. Kieboom J., Dennis J.J., Zylstra G.J., de Bont J.A.M.
    Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 700801 / S12.

Entry informationi

Entry nameiSRPB_PSEPU
AccessioniPrimary (citable) accession number: O31100
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 1, 1998
Last modified: January 20, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.