Reviewed, UniProtKB/Swiss-Prot O31097 (PHYC_BACSU)

Names and origin

Protein names

Recommended name:
    3-phytase
    EC=3.1.3.8
Alternative name(s):
    Phytate 3-phosphatase
    MYO-inositol-hexaphosphate 3-phosphohydrolase

Gene names

Name:	phyC
Synonyms:	phyB13

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the hydrolysis of inorganic orthophosphate from phytate. Only phytate, ADP, and ATP were hydrolyzed (100, 75, and 50% of the relative activity, respectively).
Catalytic activity	Myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate.
Cofactor	Calcium. Required for its activity and/or stability.
Subcellular location	Secreted.
Induction	By phytate.
Biophysicochemical properties	pH dependence: Optimum pH is 7. Temperature dependence: Optimum temperature is 55 degrees Celsius.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phytase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Propeptide	27 – 29	3			PRO_0000022055
	Chain	30 – 383	354	3-phytase		PRO_0000022056

Sequences

	Sequence		Length	Mass (Da)
	O31097-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: E9BEC2E4A48EB9CA	FASTA	383	41,923
	10 20 30 40 50 60 MNHSKTLLLT AAAGLMLTCG AVSSQAKHKL SDPYHFTVNA AAETEPVDTA GDAADDPAIW 70 80 90 100 110 120 LDPKTPQNSK LITTNKKSGL VVYSLDGKML HSYNTGKLNN VDIRYDFPLN GKKVDIAAAS 130 140 150 160 170 180 NRSEGKNTIE IYAIDGKNGT LQSMTDPDHP IATAINEVYG FTLYHSQKTG KYYAMVTGKE 190 200 210 220 230 240 GEFEQYELKA DKNGYISGKK VRAFKMNSQT EGMAADDEYG RLYIAEEDEA IWKFSAEPDG 250 260 270 280 290 300 GSNGTVIDRA DGRHLTRDIE GLTIYYAADG KGYLMASSQG NSSYAIYDRQ GKNKYVADFR 310 320 330 340 350 360 ITDGPETDGT SDTDGIDVLG FGLGPEYPFG IFVAQDGENI DHGQKANQNF KIVPWERIAD 370 380 QIGFRPLANE QVDPRKLTDR SGK

References

[1]	"Isolation, characterization, molecular gene cloning, and sequencing of a novel phytase from Bacillus subtilis." Kerovuo J., Lauraeus M., Nurminen P., Kalkkinen N., Apajalahti J. Appl. Environ. Microbiol. 64:2079-2085(1998) [PubMed: 9603817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: VTT-E-68013.
[2]	"Cloning of neutral phytase gene nphy from Bacillus subtilis and its expression in Escherichia coli." Yao B., Yuan T., Wang Y., Fan Y. Chin. J. Biotechnol. 17:11-15(2001) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 981.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF029053 Genomic DNA. Translation: AAC31775.1. AJ277890 Genomic DNA. Translation: CAB91845.1.
3D structure databases
HSSP	HSSP built from PDB template 1H6L based on UniProtKB Q938A7.
SMR	O31097. Positions 29-381.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.3.8. 150.
Family and domain databases
InterPro	IPR003431. Phytase. [Graphical view]
Pfam	PF02333. Phytase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHYC_BACSU

Accession

Primary (citable) accession number: O31097

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)