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Protein

3-phytase

Gene

phyC

Organism
Bacillus subtilis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of inorganic orthophosphate from phytate. Only phytate, ADP, and ATP were hydrolyzed (100, 75, and 50% of the relative activity, respectively).

Catalytic activityi

Myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate.PROSITE-ProRule annotation

Cofactori

Ca2+Note: Ca2+ is required for its activity and/or stability.

pH dependencei

Optimum pH is 7.

Temperature dependencei

Optimum temperature is 55 degrees Celsius.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.1.3.8. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phytase (EC:3.1.3.8)
Alternative name(s):
MYO-inositol-hexaphosphate 3-phosphohydrolase
Phytate 3-phosphatase
Gene namesi
Name:phyC
Synonyms:phyB13
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
PropeptideiPRO_000002205527 – 293
ChainiPRO_000002205630 – 3833-phytaseAdd BLAST354

Expressioni

Inductioni

By phytate.

Structurei

Secondary structure

1383
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi35 – 38Combined sources4
Beta strandi41 – 43Combined sources3
Beta strandi54 – 60Combined sources7
Helixi66 – 68Combined sources3
Beta strandi70 – 75Combined sources6
Turni76 – 78Combined sources3
Beta strandi79 – 84Combined sources6
Beta strandi89 – 93Combined sources5
Beta strandi98 – 109Combined sources12
Beta strandi112 – 121Combined sources10
Turni124 – 126Combined sources3
Beta strandi128 – 134Combined sources7
Turni136 – 138Combined sources3
Beta strandi141 – 143Combined sources3
Beta strandi161 – 165Combined sources5
Turni167 – 169Combined sources3
Beta strandi172 – 177Combined sources6
Beta strandi179 – 190Combined sources12
Beta strandi196 – 205Combined sources10
Beta strandi210 – 216Combined sources7
Turni217 – 220Combined sources4
Beta strandi221 – 226Combined sources6
Turni227 – 229Combined sources3
Beta strandi230 – 235Combined sources6
Beta strandi245 – 255Combined sources11
Beta strandi259 – 266Combined sources8
Helixi268 – 270Combined sources3
Beta strandi272 – 278Combined sources7
Helixi279 – 281Combined sources3
Beta strandi283 – 290Combined sources8
Beta strandi295 – 301Combined sources7
Beta strandi305 – 307Combined sources3
Beta strandi316 – 319Combined sources4
Beta strandi331 – 338Combined sources8
Beta strandi349 – 354Combined sources6
Helixi355 – 361Combined sources7
Helixi368 – 370Combined sources3
Helixi374 – 376Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AMRX-ray1.25A29-383[»]
3AMSX-ray2.08A29-383[»]
ProteinModelPortaliO31097.
SMRiO31097.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO31097.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 362BPPPROSITE-ProRule annotationAdd BLAST333

Sequence similaritiesi

Contains 1 BPP (beta-propeller phytase) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.120.10.20. 1 hit.
InterProiIPR003431. b_Phytase.
[Graphical view]
PfamiPF02333. Phytase. 1 hit.
[Graphical view]
PROSITEiPS51662. BP_PHYTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O31097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNHSKTLLLT AAAGLMLTCG AVSSQAKHKL SDPYHFTVNA AAETEPVDTA
60 70 80 90 100
GDAADDPAIW LDPKTPQNSK LITTNKKSGL VVYSLDGKML HSYNTGKLNN
110 120 130 140 150
VDIRYDFPLN GKKVDIAAAS NRSEGKNTIE IYAIDGKNGT LQSMTDPDHP
160 170 180 190 200
IATAINEVYG FTLYHSQKTG KYYAMVTGKE GEFEQYELKA DKNGYISGKK
210 220 230 240 250
VRAFKMNSQT EGMAADDEYG RLYIAEEDEA IWKFSAEPDG GSNGTVIDRA
260 270 280 290 300
DGRHLTRDIE GLTIYYAADG KGYLMASSQG NSSYAIYDRQ GKNKYVADFR
310 320 330 340 350
ITDGPETDGT SDTDGIDVLG FGLGPEYPFG IFVAQDGENI DHGQKANQNF
360 370 380
KIVPWERIAD QIGFRPLANE QVDPRKLTDR SGK
Length:383
Mass (Da):41,923
Last modified:January 1, 1998 - v1
Checksum:iE9BEC2E4A48EB9CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029053 Genomic DNA. Translation: AAC31775.1.
AJ277890 Genomic DNA. Translation: CAB91845.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029053 Genomic DNA. Translation: AAC31775.1.
AJ277890 Genomic DNA. Translation: CAB91845.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AMRX-ray1.25A29-383[»]
3AMSX-ray2.08A29-383[»]
ProteinModelPortaliO31097.
SMRiO31097.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.3.8. 658.

Miscellaneous databases

EvolutionaryTraceiO31097.

Family and domain databases

Gene3Di2.120.10.20. 1 hit.
InterProiIPR003431. b_Phytase.
[Graphical view]
PfamiPF02333. Phytase. 1 hit.
[Graphical view]
PROSITEiPS51662. BP_PHYTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHYC_BACIU
AccessioniPrimary (citable) accession number: O31097
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.