Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O31097

- PHYC_BACIU

UniProt

O31097 - PHYC_BACIU

Protein

3-phytase

Gene

phyC

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the hydrolysis of inorganic orthophosphate from phytate. Only phytate, ADP, and ATP were hydrolyzed (100, 75, and 50% of the relative activity, respectively).

    Catalytic activityi

    Myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate.PROSITE-ProRule annotation

    Cofactori

    Calcium. Required for its activity and/or stability.

    pH dependencei

    Optimum pH is 7.

    Temperature dependencei

    Optimum temperature is 55 degrees Celsius.

    GO - Molecular functioni

    1. 3-phytase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-phytase (EC:3.1.3.8)
    Alternative name(s):
    MYO-inositol-hexaphosphate 3-phosphohydrolase
    Phytate 3-phosphatase
    Gene namesi
    Name:phyC
    Synonyms:phyB13
    OrganismiBacillus subtilis
    Taxonomic identifieri1423 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Propeptidei27 – 293PRO_0000022055
    Chaini30 – 3833543-phytasePRO_0000022056Add
    BLAST

    Expressioni

    Inductioni

    By phytate.

    Structurei

    Secondary structure

    1
    383
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi35 – 384
    Beta strandi41 – 433
    Beta strandi54 – 607
    Helixi66 – 683
    Beta strandi70 – 756
    Turni76 – 783
    Beta strandi79 – 846
    Beta strandi89 – 935
    Beta strandi98 – 10912
    Beta strandi112 – 12110
    Turni124 – 1263
    Beta strandi128 – 1347
    Turni136 – 1383
    Beta strandi141 – 1433
    Beta strandi161 – 1655
    Turni167 – 1693
    Beta strandi172 – 1776
    Beta strandi179 – 19012
    Beta strandi196 – 20510
    Beta strandi210 – 2167
    Turni217 – 2204
    Beta strandi221 – 2266
    Turni227 – 2293
    Beta strandi230 – 2356
    Beta strandi245 – 25511
    Beta strandi259 – 2668
    Helixi268 – 2703
    Beta strandi272 – 2787
    Helixi279 – 2813
    Beta strandi283 – 2908
    Beta strandi295 – 3017
    Beta strandi305 – 3073
    Beta strandi316 – 3194
    Beta strandi331 – 3388
    Beta strandi349 – 3546
    Helixi355 – 3617
    Helixi368 – 3703
    Helixi374 – 3763

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3AMRX-ray1.25A29-383[»]
    3AMSX-ray2.08A29-383[»]
    ProteinModelPortaliO31097.
    SMRiO31097. Positions 29-381.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO31097.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 362333BPPPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 BPP (beta-propeller phytase) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.120.10.20. 1 hit.
    InterProiIPR003431. b_Phytase.
    [Graphical view]
    PfamiPF02333. Phytase. 1 hit.
    [Graphical view]
    PROSITEiPS51662. BP_PHYTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O31097-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNHSKTLLLT AAAGLMLTCG AVSSQAKHKL SDPYHFTVNA AAETEPVDTA    50
    GDAADDPAIW LDPKTPQNSK LITTNKKSGL VVYSLDGKML HSYNTGKLNN 100
    VDIRYDFPLN GKKVDIAAAS NRSEGKNTIE IYAIDGKNGT LQSMTDPDHP 150
    IATAINEVYG FTLYHSQKTG KYYAMVTGKE GEFEQYELKA DKNGYISGKK 200
    VRAFKMNSQT EGMAADDEYG RLYIAEEDEA IWKFSAEPDG GSNGTVIDRA 250
    DGRHLTRDIE GLTIYYAADG KGYLMASSQG NSSYAIYDRQ GKNKYVADFR 300
    ITDGPETDGT SDTDGIDVLG FGLGPEYPFG IFVAQDGENI DHGQKANQNF 350
    KIVPWERIAD QIGFRPLANE QVDPRKLTDR SGK 383
    Length:383
    Mass (Da):41,923
    Last modified:January 1, 1998 - v1
    Checksum:iE9BEC2E4A48EB9CA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF029053 Genomic DNA. Translation: AAC31775.1.
    AJ277890 Genomic DNA. Translation: CAB91845.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF029053 Genomic DNA. Translation: AAC31775.1 .
    AJ277890 Genomic DNA. Translation: CAB91845.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3AMR X-ray 1.25 A 29-383 [» ]
    3AMS X-ray 2.08 A 29-383 [» ]
    ProteinModelPortali O31097.
    SMRi O31097. Positions 29-381.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei O31097.

    Family and domain databases

    Gene3Di 2.120.10.20. 1 hit.
    InterProi IPR003431. b_Phytase.
    [Graphical view ]
    Pfami PF02333. Phytase. 1 hit.
    [Graphical view ]
    PROSITEi PS51662. BP_PHYTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, characterization, molecular gene cloning, and sequencing of a novel phytase from Bacillus subtilis."
      Kerovuo J., Lauraeus M., Nurminen P., Kalkkinen N., Apajalahti J.
      Appl. Environ. Microbiol. 64:2079-2085(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: VTT-E-68013.
    2. "Cloning of neutral phytase gene nphy from Bacillus subtilis and its expression in Escherichia coli."
      Yao B., Yuan T., Wang Y., Fan Y.
      Chin. J. Biotechnol. 17:11-15(2001)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 981.

    Entry informationi

    Entry nameiPHYC_BACIU
    AccessioniPrimary (citable) accession number: O31097
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3