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Reviewed, UniProtKB/Swiss-Prot O31097 (PHYC_BACSU)

Last modified June 16, 2009. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information

Names and origin

Protein namesRecommended name:
    3-phytase
    EC=3.1.3.8
Alternative name(s):
    Phytate 3-phosphatase
    MYO-inositol-hexaphosphate 3-phosphohydrolase
Gene names
Name: phyC
Synonyms: phyB13
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesBacillaceaeBacillus

Protein attributes

Sequence length383 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the hydrolysis of inorganic orthophosphate from phytate. Only phytate, ADP, and ATP were hydrolyzed (100, 75, and 50% of the relative activity, respectively).

Catalytic activity

Myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate.

Cofactor

Calcium. Required for its activity and/or stability.

Subcellular location

Secreted.

Induction

By phytate.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.

Temperature dependence:

Optimum temperature is 55 degrees Celsius.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function3-phytase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Propeptide27 – 293
PRO_0000022055
Chain30 – 3833543-phytase
PRO_0000022056

Sequences

Sequence LengthMass (Da)Tools
O31097-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: E9BEC2E4A48EB9CA

FASTA38341,923
        10         20         30         40         50         60 
MNHSKTLLLT AAAGLMLTCG AVSSQAKHKL SDPYHFTVNA AAETEPVDTA GDAADDPAIW 

        70         80         90        100        110        120 
LDPKTPQNSK LITTNKKSGL VVYSLDGKML HSYNTGKLNN VDIRYDFPLN GKKVDIAAAS 

       130        140        150        160        170        180 
NRSEGKNTIE IYAIDGKNGT LQSMTDPDHP IATAINEVYG FTLYHSQKTG KYYAMVTGKE 

       190        200        210        220        230        240 
GEFEQYELKA DKNGYISGKK VRAFKMNSQT EGMAADDEYG RLYIAEEDEA IWKFSAEPDG 

       250        260        270        280        290        300 
GSNGTVIDRA DGRHLTRDIE GLTIYYAADG KGYLMASSQG NSSYAIYDRQ GKNKYVADFR 

       310        320        330        340        350        360 
ITDGPETDGT SDTDGIDVLG FGLGPEYPFG IFVAQDGENI DHGQKANQNF KIVPWERIAD 

       370        380 
QIGFRPLANE QVDPRKLTDR SGK 

« Hide

References

[1]"Isolation, characterization, molecular gene cloning, and sequencing of a novel phytase from Bacillus subtilis."
Kerovuo J., Lauraeus M., Nurminen P., Kalkkinen N., Apajalahti J.
Appl. Environ. Microbiol. 64:2079-2085(1998) [PubMed: 9603817] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: VTT-E-68013.
[2]"Cloning of neutral phytase gene nphy from Bacillus subtilis and its expression in Escherichia coli."
Yao B., Yuan T., Wang Y., Fan Y.
Chin. J. Biotechnol. 17:11-15(2001)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 981.

Cross-references

Sequence databases

AF029053 Genomic DNA. Translation: AAC31775.1.
AJ277890 Genomic DNA. Translation: CAB91845.1.

3D structure databases

HSSPHSSP built from PDB template 1H6L based on UniProtKB Q938A7.
SMRO31097. Positions 29-381.
ModBaseSearch...

Enzyme and pathway databases

BRENDA3.1.3.8. 150.

Family and domain databases

InterProIPR003431. Phytase.
[Graphical view]
PfamPF02333. Phytase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePHYC_BACSU
AccessionPrimary (citable) accession number: O31097
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information