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Protein

Type-2 restriction enzyme SacI

Gene

sacIR

Organism
Streptomyces achromogenes
Status
Reviewed-Annotation score: -Protein predictedi

Functioni

Recognizes the double-stranded sequence GAGCTC and cleaves after T-5.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system

Enzyme and pathway databases

BRENDAi3.1.21.4 5915

Protein family/group databases

REBASEi1578 SacI

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme SacI (EC:3.1.21.4)
Short name:
R.SacI
Alternative name(s):
Endonuclease SacI
Type II restriction enzyme SacI
Gene namesi
Name:sacIR
OrganismiStreptomyces achromogenes
Taxonomic identifieri67255 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773641 – 358Type-2 restriction enzyme SacIAdd BLAST358

Proteomic databases

PRIDEiO31074

Structurei

3D structure databases

SMRiO31074
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Family and domain databases

InterProiView protein in InterPro
IPR019066 Restrct_endonuc_II_SacI
PfamiView protein in Pfam
PF09566 RE_SacI, 1 hit

Sequencei

Sequence statusi: Complete.

O31074-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGITIKKSTA EQVLRKAYEA AASDDVFLED WIFLATSLRE VDAPRTYTAA
60 70 80 90 100
LVTALLARAC DDRVDPRSIK EKYDDRAFSL RTLCHGVVVP MSVELGFDLG
110 120 130 140 150
ATGREPINNQ PFFRYDQYSE IVRVQTKARP YLDRVSSALA RVDEEDYSTE
160 170 180 190 200
ESFRALVAVL AVCISVANKK QRVAVGSAIV EASLIAETQS FVVSGHDVPR
210 220 230 240 250
KLQACVAAGL DMVYSEVVSR RINDPSRDFP GDVQVILDGD PLLTVEVRGK
260 270 280 290 300
SVSWEGLEQF VSSATYAGFR RVALMVDAAS HVSLMSADDL TSALERKYEC
310 320 330 340 350
IVKVNESVSS FLRDVFVWSP RDVHSILSAF PEAMYRRMIE IEVREPELDR

WAEIFPET
Length:358
Mass (Da):39,965
Last modified:January 1, 1998 - v1
Checksum:iEBF68CB8037EBF13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027867 Genomic DNA Translation: AAC97119.1

Similar proteinsi

Entry informationi

Entry nameiT2S1_STRAH
AccessioniPrimary (citable) accession number: O31074
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 47 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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