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O31074

- T2S1_STRAH

UniProt

O31074 - T2S1_STRAH

Protein

Type-2 restriction enzyme SacI

Gene

sacIR

Organism
Streptomyces achromogenes
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 43 (16 Apr 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GAGCTC and cleaves after T-5.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1578. SacI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme SacI (EC:3.1.21.4)
    Short name:
    R.SacI
    Alternative name(s):
    Endonuclease SacI
    Type II restriction enzyme SacI
    Gene namesi
    Name:sacIR
    OrganismiStreptomyces achromogenes
    Taxonomic identifieri67255 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 358358Type-2 restriction enzyme SacIPRO_0000077364Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR019066. Restrct_endonuc_II_SacI.
    [Graphical view]
    PfamiPF09566. RE_SacI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O31074-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGITIKKSTA EQVLRKAYEA AASDDVFLED WIFLATSLRE VDAPRTYTAA    50
    LVTALLARAC DDRVDPRSIK EKYDDRAFSL RTLCHGVVVP MSVELGFDLG 100
    ATGREPINNQ PFFRYDQYSE IVRVQTKARP YLDRVSSALA RVDEEDYSTE 150
    ESFRALVAVL AVCISVANKK QRVAVGSAIV EASLIAETQS FVVSGHDVPR 200
    KLQACVAAGL DMVYSEVVSR RINDPSRDFP GDVQVILDGD PLLTVEVRGK 250
    SVSWEGLEQF VSSATYAGFR RVALMVDAAS HVSLMSADDL TSALERKYEC 300
    IVKVNESVSS FLRDVFVWSP RDVHSILSAF PEAMYRRMIE IEVREPELDR 350
    WAEIFPET 358
    Length:358
    Mass (Da):39,965
    Last modified:January 1, 1998 - v1
    Checksum:iEBF68CB8037EBF13
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF027867 Genomic DNA. Translation: AAC97119.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF027867 Genomic DNA. Translation: AAC97119.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1578. SacI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019066. Restrct_endonuc_II_SacI.
    [Graphical view ]
    Pfami PF09566. RE_SacI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
      Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
      Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 12767 / CBS 458.68 / DSM 40028 / JCM 4121 / NBRC 12735 / NRRL B-2120.

    Entry informationi

    Entry nameiT2S1_STRAH
    AccessioniPrimary (citable) accession number: O31074
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: January 1, 1998
    Last modified: April 16, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3