Reviewed, UniProtKB/Swiss-Prot O31074 (T2S1_STRAH)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme SacI
      Short name=R.SacI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme SacI
    Endonuclease SacI

Gene names

Name:

sacIR

Organism

Streptomyces achromogenes

Taxonomic identifier

67255 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GAGCTC and cleaves after T-5.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Type-2 restriction enzyme SacI		PRO_0000077364

Sequences

	Sequence		Length	Mass (Da)
	O31074-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: EBF68CB8037EBF13	FASTA	358	39,965
	10 20 30 40 50 60 MGITIKKSTA EQVLRKAYEA AASDDVFLED WIFLATSLRE VDAPRTYTAA LVTALLARAC 70 80 90 100 110 120 DDRVDPRSIK EKYDDRAFSL RTLCHGVVVP MSVELGFDLG ATGREPINNQ PFFRYDQYSE 130 140 150 160 170 180 IVRVQTKARP YLDRVSSALA RVDEEDYSTE ESFRALVAVL AVCISVANKK QRVAVGSAIV 190 200 210 220 230 240 EASLIAETQS FVVSGHDVPR KLQACVAAGL DMVYSEVVSR RINDPSRDFP GDVQVILDGD 250 260 270 280 290 300 PLLTVEVRGK SVSWEGLEQF VSSATYAGFR RVALMVDAAS HVSLMSADDL TSALERKYEC 310 320 330 340 350 IVKVNESVSS FLRDVFVWSP RDVHSILSAF PEAMYRRMIE IEVREPELDR WAEIFPET

References

[1]

"Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli." Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II Mol. Gen. Genet. 260:226-231(1998) [PubMed: 9862476] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 12767 / CBS 458.68 / DSM 40028 / IFO 12735 / JCM 4121.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF027867 Genomic DNA. Translation: AAC97119.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1578. SacI.
Enzyme and pathway databases
BRENDA	3.1.21.4. 1632.
Family and domain databases
InterPro	IPR019066. Restrict_endonuc_II_SacI. [Graphical view]
Pfam	PF09566. RE_SacI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2S1_STRAH

Accession

Primary (citable) accession number: O31074

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)