O31074 (T2S1_STRAH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme SacI
Short name=R.SacI
EC=3.1.21.4

Alternative name(s):
Endonuclease SacI
Type II restriction enzyme SacI

Gene names

Name:

sacIR

Organism

Streptomyces achromogenes

Taxonomic identifier

67255 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence GAGCTC and cleaves after T-5.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Type-2 restriction enzyme SacI		PRO_0000077364

Sequences

	Sequence		Length	Mass (Da)
	O31074 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: EBF68CB8037EBF13	FASTA	358	39,965
	10 20 30 40 50 60 MGITIKKSTA EQVLRKAYEA AASDDVFLED WIFLATSLRE VDAPRTYTAA LVTALLARAC 70 80 90 100 110 120 DDRVDPRSIK EKYDDRAFSL RTLCHGVVVP MSVELGFDLG ATGREPINNQ PFFRYDQYSE 130 140 150 160 170 180 IVRVQTKARP YLDRVSSALA RVDEEDYSTE ESFRALVAVL AVCISVANKK QRVAVGSAIV 190 200 210 220 230 240 EASLIAETQS FVVSGHDVPR KLQACVAAGL DMVYSEVVSR RINDPSRDFP GDVQVILDGD 250 260 270 280 290 300 PLLTVEVRGK SVSWEGLEQF VSSATYAGFR RVALMVDAAS HVSLMSADDL TSALERKYEC 310 320 330 340 350 IVKVNESVSS FLRDVFVWSP RDVHSILSAF PEAMYRRMIE IEVREPELDR WAEIFPET

References

[1]

"Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli." Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 12767 / CBS 458.68 / DSM 40028 / JCM 4121 / NBRC 12735 / NRRL B-2120.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF027867 Genomic DNA. Translation: AAC97119.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1578. SacI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019066. Restrct_endonuc_II_SacI. [Graphical view]
Pfam	PF09566. RE_SacI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2S1_STRAH

Accession

Primary (citable) accession number: O31074

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program