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O31074 (T2S1_STRAH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme SacI

Short name=R.SacI
EC=3.1.21.4
Alternative name(s):
Endonuclease SacI
Type II restriction enzyme SacI
Gene names
Name:sacIR
OrganismStreptomyces achromogenes
Taxonomic identifier67255 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GAGCTC and cleaves after T-5.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 358358Type-2 restriction enzyme SacI
PRO_0000077364

Sequences

Sequence LengthMass (Da)Tools
O31074 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: EBF68CB8037EBF13

FASTA35839,965
        10         20         30         40         50         60 
MGITIKKSTA EQVLRKAYEA AASDDVFLED WIFLATSLRE VDAPRTYTAA LVTALLARAC 

        70         80         90        100        110        120 
DDRVDPRSIK EKYDDRAFSL RTLCHGVVVP MSVELGFDLG ATGREPINNQ PFFRYDQYSE 

       130        140        150        160        170        180 
IVRVQTKARP YLDRVSSALA RVDEEDYSTE ESFRALVAVL AVCISVANKK QRVAVGSAIV 

       190        200        210        220        230        240 
EASLIAETQS FVVSGHDVPR KLQACVAAGL DMVYSEVVSR RINDPSRDFP GDVQVILDGD 

       250        260        270        280        290        300 
PLLTVEVRGK SVSWEGLEQF VSSATYAGFR RVALMVDAAS HVSLMSADDL TSALERKYEC 

       310        320        330        340        350 
IVKVNESVSS FLRDVFVWSP RDVHSILSAF PEAMYRRMIE IEVREPELDR WAEIFPET 

« Hide

References

[1]"Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 12767 / CBS 458.68 / DSM 40028 / JCM 4121 / NBRC 12735 / NRRL B-2120.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF027867 Genomic DNA. Translation: AAC97119.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1578. SacI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019066. Restrct_endonuc_II_SacI.
[Graphical view]
PfamPF09566. RE_SacI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2S1_STRAH
AccessionPrimary (citable) accession number: O31074
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries