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O31074

- T2S1_STRAH

UniProt

O31074 - T2S1_STRAH

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Protein

Type-2 restriction enzyme SacI

Gene

sacIR

Organism
Streptomyces achromogenes
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi

Functioni

Recognizes the double-stranded sequence GAGCTC and cleaves after T-5.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1578. SacI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme SacI (EC:3.1.21.4)
Short name:
R.SacI
Alternative name(s):
Endonuclease SacI
Type II restriction enzyme SacI
Gene namesi
Name:sacIR
OrganismiStreptomyces achromogenes
Taxonomic identifieri67255 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Type-2 restriction enzyme SacIPRO_0000077364Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019066. Restrct_endonuc_II_SacI.
[Graphical view]
PfamiPF09566. RE_SacI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O31074-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGITIKKSTA EQVLRKAYEA AASDDVFLED WIFLATSLRE VDAPRTYTAA
60 70 80 90 100
LVTALLARAC DDRVDPRSIK EKYDDRAFSL RTLCHGVVVP MSVELGFDLG
110 120 130 140 150
ATGREPINNQ PFFRYDQYSE IVRVQTKARP YLDRVSSALA RVDEEDYSTE
160 170 180 190 200
ESFRALVAVL AVCISVANKK QRVAVGSAIV EASLIAETQS FVVSGHDVPR
210 220 230 240 250
KLQACVAAGL DMVYSEVVSR RINDPSRDFP GDVQVILDGD PLLTVEVRGK
260 270 280 290 300
SVSWEGLEQF VSSATYAGFR RVALMVDAAS HVSLMSADDL TSALERKYEC
310 320 330 340 350
IVKVNESVSS FLRDVFVWSP RDVHSILSAF PEAMYRRMIE IEVREPELDR

WAEIFPET
Length:358
Mass (Da):39,965
Last modified:January 1, 1998 - v1
Checksum:iEBF68CB8037EBF13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027867 Genomic DNA. Translation: AAC97119.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027867 Genomic DNA. Translation: AAC97119.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1578. SacI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR019066. Restrct_endonuc_II_SacI.
[Graphical view ]
Pfami PF09566. RE_SacI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
    Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
    Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 12767 / CBS 458.68 / DSM 40028 / JCM 4121 / NBRC 12735 / NRRL B-2120.

Entry informationi

Entry nameiT2S1_STRAH
AccessioniPrimary (citable) accession number: O31074
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3