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O31073

- MTS1_STRAH

UniProt

O31073 - MTS1_STRAH

Protein

Modification methylase SacI

Gene

sacIM

Organism
Streptomyces achromogenes
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GAGCTC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the SacI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei96 – 961PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3489. M.SacI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase SacI (EC:2.1.1.37)
    Short name:
    M.SacI
    Alternative name(s):
    Cytosine-specific methyltransferase SacI
    Gene namesi
    Name:sacIM
    OrganismiStreptomyces achromogenes
    Taxonomic identifieri67255 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 390390Modification methylase SacIPRO_0000087909Add
    BLAST

    Proteomic databases

    PRIDEiO31073.

    Structurei

    3D structure databases

    ProteinModelPortaliO31073.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 371367SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O31073-1 [UniParc]FASTAAdd to Basket

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    MNHELPVISL FSGAGGLDCA IESCAEPPLV QDGSGSPLRV AVATDYEQTA    50
    LDTLSANFPH TKTLCGDIQT IPTAELLEAG GLKPGDPTLV IGGPPCTPFS 100
    KSGFWIEEKR NSADPNASLL DEYVRVVRES KPEAFILENV QGLTYKTHQA 150
    QFDRLIAGLK DAGYNPTFRV LLAAEYGVPQ LRRRVFVVGR RDGKAFHFPE 200
    TTHSGESERD RVIDHTKIPF TSLREALAGL PDVPEAGEVV EGTYAELAAE 250
    VPPGQNYLWH TDRYGGRNEF KWRSRYWTFL LKADPDRPST TLQAQPGPWV 300
    GPFHWENVKN ANGEERARRF RVAEMKRIMT FPDEFVFTGV KREVQRQIGN 350
    PVPVELGKVV VRALMEQLGY LDSRGTTIPS QAGHEQLELI 390
    Length:390
    Mass (Da):43,293
    Last modified:January 1, 1998 - v1
    Checksum:iC7470D97BADFCE87
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF027867 Genomic DNA. Translation: AAC97118.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF027867 Genomic DNA. Translation: AAC97118.1 .

    3D structure databases

    ProteinModelPortali O31073.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3489. M.SacI.

    Proteomic databases

    PRIDEi O31073.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
      Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
      Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 12767 / CBS 458.68 / DSM 40028 / JCM 4121 / NBRC 12735 / NRRL B-2120.

    Entry informationi

    Entry nameiMTS1_STRAH
    AccessioniPrimary (citable) accession number: O31073
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3