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O31073

- MTS1_STRAH

UniProt

O31073 - MTS1_STRAH

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Protein

Modification methylase SacI

Gene
sacIM
Organism
Streptomyces achromogenes
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GAGCTC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the SacI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei96 – 961 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3489. M.SacI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase SacI (EC:2.1.1.37)
Short name:
M.SacI
Alternative name(s):
Cytosine-specific methyltransferase SacI
Gene namesi
Name:sacIM
OrganismiStreptomyces achromogenes
Taxonomic identifieri67255 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Modification methylase SacIPRO_0000087909Add
BLAST

Proteomic databases

PRIDEiO31073.

Structurei

3D structure databases

ProteinModelPortaliO31073.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 371367SAM-dependent MTase C5-typeAdd
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O31073-1 [UniParc]FASTAAdd to Basket

« Hide

MNHELPVISL FSGAGGLDCA IESCAEPPLV QDGSGSPLRV AVATDYEQTA    50
LDTLSANFPH TKTLCGDIQT IPTAELLEAG GLKPGDPTLV IGGPPCTPFS 100
KSGFWIEEKR NSADPNASLL DEYVRVVRES KPEAFILENV QGLTYKTHQA 150
QFDRLIAGLK DAGYNPTFRV LLAAEYGVPQ LRRRVFVVGR RDGKAFHFPE 200
TTHSGESERD RVIDHTKIPF TSLREALAGL PDVPEAGEVV EGTYAELAAE 250
VPPGQNYLWH TDRYGGRNEF KWRSRYWTFL LKADPDRPST TLQAQPGPWV 300
GPFHWENVKN ANGEERARRF RVAEMKRIMT FPDEFVFTGV KREVQRQIGN 350
PVPVELGKVV VRALMEQLGY LDSRGTTIPS QAGHEQLELI 390
Length:390
Mass (Da):43,293
Last modified:January 1, 1998 - v1
Checksum:iC7470D97BADFCE87
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF027867 Genomic DNA. Translation: AAC97118.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF027867 Genomic DNA. Translation: AAC97118.1 .

3D structure databases

ProteinModelPortali O31073.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3489. M.SacI.

Proteomic databases

PRIDEi O31073.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
    Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
    Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 12767 / CBS 458.68 / DSM 40028 / JCM 4121 / NBRC 12735 / NRRL B-2120.

Entry informationi

Entry nameiMTS1_STRAH
AccessioniPrimary (citable) accession number: O31073
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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