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O31073 (MTS1_STRAH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase SacI

Short name=M.SacI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase SacI
Gene names
Name:sacIM
OrganismStreptomyces achromogenes
Taxonomic identifier67255 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length390 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GAGCTC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the SacI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 390390Modification methylase SacI
PRO_0000087909

Regions

Domain5 – 371367SAM-dependent MTase C5-type

Sites

Active site961 By similarity

Sequences

Sequence LengthMass (Da)Tools
O31073 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: C7470D97BADFCE87

FASTA39043,293
        10         20         30         40         50         60 
MNHELPVISL FSGAGGLDCA IESCAEPPLV QDGSGSPLRV AVATDYEQTA LDTLSANFPH 

        70         80         90        100        110        120 
TKTLCGDIQT IPTAELLEAG GLKPGDPTLV IGGPPCTPFS KSGFWIEEKR NSADPNASLL 

       130        140        150        160        170        180 
DEYVRVVRES KPEAFILENV QGLTYKTHQA QFDRLIAGLK DAGYNPTFRV LLAAEYGVPQ 

       190        200        210        220        230        240 
LRRRVFVVGR RDGKAFHFPE TTHSGESERD RVIDHTKIPF TSLREALAGL PDVPEAGEVV 

       250        260        270        280        290        300 
EGTYAELAAE VPPGQNYLWH TDRYGGRNEF KWRSRYWTFL LKADPDRPST TLQAQPGPWV 

       310        320        330        340        350        360 
GPFHWENVKN ANGEERARRF RVAEMKRIMT FPDEFVFTGV KREVQRQIGN PVPVELGKVV 

       370        380        390 
VRALMEQLGY LDSRGTTIPS QAGHEQLELI 

« Hide

References

[1]"Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 12767 / CBS 458.68 / DSM 40028 / JCM 4121 / NBRC 12735 / NRRL B-2120.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF027867 Genomic DNA. Translation: AAC97118.1.

3D structure databases

ProteinModelPortalO31073.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3489. M.SacI.

Proteomic databases

PRIDEO31073.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTS1_STRAH
AccessionPrimary (citable) accession number: O31073
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries