Reviewed, UniProtKB/Swiss-Prot O31067 (HEMN_THEVL)

Names and origin

Protein names

Recommended name:
    Oxygen-independent coproporphyrinogen-III oxidase
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.99.22

Gene names

Name:

hemN

Organism

Thermosynechococcus vulcanus (Synechococcus vulcanus)

Taxonomic identifier

32053 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Thermosynechococcus

Protein attributes

Sequence length	325 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX By similarity.
Catalytic activity	Coproporphyrinogen-III + 2 S-adenosyl-L-methionine = protoporphyrinogen-IX + 2 CO2 + 2 L-methionine + 2 5'-deoxyadenosine.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (AdoMet route): step 1/1.
Subunit structure	Monomer.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the anaerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW coproporphyrinogen dehydrogenase activity Inferred from electronic annotation. Source: EC coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 325	›325	Oxygen-independent coproporphyrinogen-III oxidase		PRO_0000109953
Sites
	Binding site	13	1	S-adenosyl-L-methionine 1 By similarity
	Binding site	40	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	52	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	77	1	S-adenosyl-L-methionine 2 By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	O31067-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A4011FB51C67732B	FASTA	325	36,817
	10 20 30 40 50 60 AFLMTKDAEI SIEVSPRYIN GDYFLRGRKL GFNRISFGVQ DFDPQVQLAV NRVQRETMFF 70 80 90 100 110 120 QVMEWIRAAE FESVNIDLIY GLPYQTVQSF EATIAKTLRL DPDRIAVFNF AYLPNLKPIQ 130 140 150 160 170 180 KRIDPTTLPD SATKLTILQR VIERLTSQGY RYIGMDHFAK PTDELAIAQR SGDLKRNFQG 190 200 210 220 230 240 YTTLPTADLI GFGLTSISML QAAYAQNQKH LATYFSDVAA GHHGPQECGF NCTVEDLLRR 250 260 270 280 290 300 TIIMELMCQF SLDKGAIARQ FNLDFDAYFA SELAALRELA ADGLLHLGRD RLEVTPVGRL 310 320 LIRNITAVFD AYLQQKSGRT FSKAI

References

[1]	Los D.A. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF027176 Genomic DNA. Translation: AAB84028.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.99.22. 276791.
Family and domain databases
InterPro	IPR006638. Elp3/MiaB/NifB. IPR004558. HemN. IPR010723. HemN_C. IPR007197. Radical_SAM. [Graphical view]
Pfam	PF06969. HemN_C. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00538. hemN. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HEMN_THEVL

Accession

Primary (citable) accession number: O31067

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)