O31066 (KATG_CAUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	CC_3043

Organism

Caulobacter crescentus (Caulobacter vibrioides)

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. Important for stationary phase survival. Ref.2
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.
Sequence caution	The sequence AAC45850.1 differs from that shown. Reason: Erroneous initiation. The sequence AAK25005.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 727	727	Catalase-peroxidase HAMAP MF_01961		PRO_0000055568
Sites
	Active site	96	1	Proton acceptor By similarity
	Metal binding	258	1	Iron (heme axial ligand) By similarity
	Site	92	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	95 ↔ 217		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-243) By similarity
	Cross-link	217 ↔ 243		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-95) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O31066 [UniParc]. Last modified November 25, 2008. Version 3. Checksum: 8E6AB7EA0B930516	FASTA	727	79,012
	10 20 30 40 50 60 MDAKVEDNIA GKCPMGHGRG PANRDWWPQS LRLEGLNQHA PRSNPMGEAF DYAEAFKSLD 70 80 90 100 110 120 LDAVVSDLHA LMTDSQEWWP ADFGHYGGLF IRLAWHAAGT YRITDGRGGA GGGQQRFAPL 130 140 150 160 170 180 NSWPDNTNLD KARRLLWPIK QKYGAKLSWA DLYVLVGNVA LESMGFKTFG FAGGRADQWE 190 200 210 220 230 240 PEELYWGPES TWLDDKRYSG ERELDSPLGA VQMGLIYVNP EGPNGNPDPL ASARDIRETF 250 260 270 280 290 300 ARMAMNDEET VALIAGGHTF GKAHGAGDAS LVGVEPEGGA IEAQGFGWAS KHGTGKGPDA 310 320 330 340 350 360 ITGGPEVIWT QTPTRWSNHF FDNLFKYEWE LTQSPAGAKQ WQAKNAPADI PDAFDPNKTH 370 380 390 400 410 420 VPRMLTSDLA LRFDPAYEKI SRRFYENPDQ FADAFARAWF KLTHRDMGPI GRYLGPLVPK 430 440 450 460 470 480 EELIWQDPIP AVDHPLADDK DIAALKAKIL ATGLSASDLV STAWASASTY RQSDKRGGAN 490 500 510 520 530 540 GARIRLAPQK DWAVNNPPVL AKVLAALEGV QKDFNASAGG GKKISLADLI VLGGAAAIEK 550 560 570 580 590 600 AAKDAGTSVT VPFAPGRMDA SAEQTDAHSF EALEPRSDGF RNYRGPGKHY MAPEEALVDR 610 620 630 640 650 660 AQLLGLSGPE LTVLVGGLRV LGANADGSKD GVFTNRPGAL SNDFFVNLLS METTWSPTAA 670 680 690 700 710 720 NAFAGHDRKS SEPRWTATRV DLIFGSHAEL RAFAEVYACA DSQEKFVCDF VTAWNKVMNA DRLDLAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.
[2]	"Catalase-peroxidase of Caulobacter crescentus: function and role in stationary-phase survival." Steinman H.M., Fareed F., Weinstein L. J. Bacteriol. 179:6831-6836(1997) [PubMed: 9352936] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-484, FUNCTION. Strain: ATCC 19089 / CB15.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005673 Genomic DNA. Translation: AAK25005.1. Different initiation. AF027168 Genomic DNA. Translation: AAC45850.1. Different initiation.
PIR	A87626. T45480.
RefSeq	NP_421837.1. NC_002696.2.
3D structure databases
ModBase	Search...
Protein family/group databases
PeroxiBase	2349. CcrCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	943888.
GenomeReviews	Gene locus CC_3043 in contig AE005673_GR.
KEGG	ccr:CC_3043.
NMPDR	fig|190650.1.peg.3017.
PATRIC	21303106. VBICauCre124313_3047.
TIGR	CC_3043.
Phylogenomic databases
HOGENOM	HBG285610.
OMA	GPEVIWT.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_CAUCR

Accession

Primary (citable) accession number: O31066

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 91 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program