Reviewed, UniProtKB/Swiss-Prot O30973 (TRXB_MYCSM)

Names and origin

Protein names

Recommended name:
    Thioredoxin reductase
      Short name=TRXR
    EC=1.8.1.9

Gene names

Name:

trxB

Organism

Mycobacterium smegmatis

Taxonomic identifier

1772 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW removal of superoxide radicals Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Thioredoxin reductase		PRO_0000166740
Regions
	Nucleotide binding	37 – 44	8	FAD By similarity
	Nucleotide binding	280 – 289	10	FAD By similarity
Amino acid modifications
	Disulfide bond	137 ↔ 140		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	O30973-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 3EC3896EADCF6493	FASTA	311	33,612
	10 20 30 40 50 60 MSTSQTVHDV IIIGSGPAGY TAAIYAARAQ LKPLVFEGTQ FGGALMTTTE VENYPGFREG 70 80 90 100 110 120 ITGPELMDQM REQALRFRAD LRMEDVDAVQ LEGPVKTVVV GDETHQARAV ILAMGAAARH 130 140 150 160 170 180 LGVPGEEALT GMGVSTCATC DGFFFRDQDI VVVGGGDSAM EEATFLTRFA RSVTLIHRRD 190 200 210 220 230 240 EFRASKIMLE RARANEKITF LTNTEITQIE GDPKVTGVRL RDTVTGEESK LDVTGVFVAI 250 260 270 280 290 300 GHDPRSELVR GQVELDDEGY VKVQGRTTYT SLDGVFAAGD LVDHTYRQAI TAAGSGCAAS 310 IDAERWLAEQ D

References

[1]	"Molecular characterization of the thioredoxin system of Mycobacterium smegmatis." Asano R.L., Davies J. Res. Microbiol. 149:567-576(1998) [PubMed: 9795994] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 607 / DSM 43465 / mc(2)6 / NCTC 7017 / NRRL B-692.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF023161 Genomic DNA. Translation: AAB80939.1.
3D structure databases
HSSP	HSSP built from PDB template 1VDC based on UniProtKB Q39243.
SMR	O30973. Positions 7-310.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.8.1.9. 259.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR005982. Thioredox_Rdtase. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01292. TRX_reduct. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXB_MYCSM

Accession

Primary (citable) accession number: O30973

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)