O30917 (SIGE_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chaperone protein SigE

Gene names

Name:	sigE
Synonyms:	pipC
Ordered Locus Names:	STM1090

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	113 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Molecular chaperone required for SopB/SigD stabilization and secretion. Ref.1 Ref.4
Subunit structure	Homodimer or higher-order oligomers.
Subcellular location	Cytoplasm Probable.
Induction	Transcriptionally regulated by InvF and SicA. Also regulated by SirA. Ref.1 Ref.3
Sequence similarities	Belongs to the IpgE/SigE chaperone family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 113

113

Chaperone protein SigE

PRO_0000160575

Secondary structure

1

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113

Helix Strand Turn

Details...

Helix

4 – 11

8

Beta strand

24 – 26

3

Beta strand

29 – 35

7

Beta strand

37 – 48

12

Helix

53 – 65

13

Beta strand

66 – 72

7

Beta strand

78 – 86

9

Helix

91 – 112

22

Sequences

	Sequence		Length	Mass (Da)
	O30917 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 34DEE3AB3944560E	FASTA	113	12,751
	10 20 30 40 50 60 MESLLNRLYD ALGLDAPEDE PLLIIDDGIQ VYFNESDHTL EMCCPFMPLP DDILTLQHFL 70 80 90 100 110 RLNYTSAVTI GADADNTALV ALYRLPQTST EEEALTGFEL FISNVKQLKE HYA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel Salmonella invasion locus homologous to Shigella ipgDE." Hong K.H., Miller V.L. J. Bacteriol. 180:1793-1802(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, REGULATION BY SIRA. Strain: ATCC 14028s / SGSG 2262.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[3]	"The putative invasion protein chaperone SicA acts together with InvF to activate the expression of Salmonella typhimurium virulence genes." Darwin K.H., Miller V.L. Mol. Microbiol. 35:949-960(2000) [PubMed] [Europe PMC] [Abstract] Cited for: REGULATION BY INVF AND SICA. Strain: ATCC 14028s / SGSG 2262, LT2 and SL1344.
[4]	"SigE is a chaperone for the Salmonella enterica serovar Typhimurium invasion protein SigD." Darwin K.H., Robinson L.S., Miller V.L. J. Bacteriol. 183:1452-1454(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: ATCC 14028s / SGSG 2262.
[5]	"Structural and biochemical characterization of the type III secretion chaperones CesT and SigE." Luo Y., Bertero M.G., Frey E.A., Pfuetzner R.A., Wenk M.R., Creagh L., Marcus S.L., Lim D., Sicheri F., Kay C., Haynes C., Finlay B.B., Strynadka N.C.J. Nat. Struct. Biol. 8:1031-1036(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF021817 Genomic DNA. Translation: AAC46235.1.
AE006468 Genomic DNA. Translation: AAL20022.1.

RefSeq

NP_460063.1. NC_003197.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K3S	X-ray	1.90	A/B	1-113	[»]

ProteinModelPortal

O30917.

SMR

O30917. Positions 1-113.

ModBase

Search...

Protein-protein interaction databases

STRING

99287.STM1090.

Proteomic databases

PaxDb

O30917.

PRIDE

O30917.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAL20022; AAL20022; STM1090.

GeneID

1252608.

KEGG

stm:STM1090.

PATRIC

32380617. VBISalEnt20916_1154.

Phylogenomic databases

eggNOG

NOG67978.

HOGENOM

HOG000028304.

OMA

LMLEMCC.

ProtClustDB

PRK15202.

Family and domain databases

InterPro

IPR013095. T3SS_chaperone.
[Graphical view]

Pfam

PF07824. Chaperone_III. 1 hit.
[Graphical view]

PIRSF

PIRSF034754. T3SS_chaperone. 1 hit.

ProDom

PD030752. T3SS_chaperone. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other

EvolutionaryTrace

O30917.

Entry information

Entry name

SIGE_SALTY

Accession

Primary (citable) accession number: O30917
Secondary accession number(s): Q7CQS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program