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Protein

Inositol phosphate phosphatase SopB

Gene

sopB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns 3-P and prevents the transition of PtdIns 3-P to PtdIns 3,5-P2. It is one of the known effectors injected by Salmonella into the host cell and is required for invasion and for an efficient generation and maintenance of Salmonella-containing vacuole (SVC). Alteration of the phosphoinositide composition of the plasma membrane causes membrane ruffling and actin cytoskeleton rearrangements. The persistence of PtdIns 3-P diverts the SCV from the endocytic pathway resulting in enlarged vesicles, which are essential to create a favorable environment where Salmonella can replicate and avoid immune defenses of the host cell.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei460 – 4601Sequence analysis

GO - Molecular functioni

  • lipid phosphatase activity Source: AgBase

GO - Biological processi

  • dephosphorylation Source: GOC
  • negative regulation of cell growth Source: AgBase
  • negative regulation of signaling Source: AgBase
  • pathogenesis Source: UniProtKB-KW
  • positive regulation of DNA replication Source: AgBase
  • regulation of protein kinase activity Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15186.
SENT99287:GCTI-1100-MONOMER.
BRENDAi3.1.3.78. 5542.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol phosphate phosphatase SopB (EC:3.1.3.-)
Alternative name(s):
Effector protein SopB
Gene namesi
Name:sopB
Synonyms:sigD
Ordered Locus Names:STM1091
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Secreted By similarity

  • Note: Secreted via the type III secretion system 1 (SPI-1 TTSS).By similarity

GO - Cellular componenti

  • cell periphery Source: AgBase
  • symbiont-containing vacuole Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi460 – 4601C → S: Loss of activity. 1 Publication
Mutagenesisi525 – 5251K → A: Decrease in activity. 1 Publication
Mutagenesisi528 – 5281K → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 561561Inositol phosphate phosphatase SopBPRO_0000220495Add
BLAST

Proteomic databases

PaxDbiO30916.
PRIDEiO30916.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC42P609535EBI-11167349,EBI-81752From a different organism.

Protein-protein interaction databases

IntActiO30916. 1 interaction.
MINTiMINT-157109.
STRINGi99287.STM1091.

Structurei

Secondary structure

1
561
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi49 – 524Combined sources
Helixi59 – 624Combined sources
Beta strandi69 – 724Combined sources
Helixi73 – 9018Combined sources
Turni91 – 933Combined sources
Helixi96 – 10712Combined sources
Helixi108 – 1103Combined sources
Helixi118 – 14225Combined sources
Helixi147 – 16620Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DIDX-ray2.35B30-181[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi460 – 4667CX5R motif

Domaini

Contains the consensus sequence Cys-X(5)-Arg characteristic of Mg-independent phosphatases.

Sequence similaritiesi

Belongs to the phosphatase IpgD/SopB family.Curated

Phylogenomic databases

eggNOGiENOG4108N4M. Bacteria.
ENOG410Y7WM. LUCA.
HOGENOMiHOG000028303.
KOiK13085.
OMAiCWNCKSG.
OrthoDBiEOG67DPGJ.

Family and domain databases

InterProiIPR008108. IpgD/SopB.
[Graphical view]
PfamiPF05925. IpgD. 1 hit.
[Graphical view]
PRINTSiPR01734. TYPE3OMBPROT.

Sequencei

Sequence statusi: Complete.

O30916-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIQSFYHSA SLKTQEAFKS LQKTLYNGMQ ILSGQGKAPA KAPDARPEII
60 70 80 90 100
VLREPGATWG NYLQHQKASN HSLHNLYNLQ RDLLTVAATV LGKQDPVLTS
110 120 130 140 150
MANQMELAKV KADRPATKQE EAAAKALKKN LIELIAARTQ QQDGLPAKEA
160 170 180 190 200
HRFAAVAFRD AQVKQLNNQP WQTIKNTLTH NGHHYTNTQL PAAEMKIGAK
210 220 230 240 250
DIFPSAYEGK GVCSWDTKNI HHANNLWMST VSVHEDGKDK TLFCGIRHGV
260 270 280 290 300
LSPYHEKDPL LRHVGAENKA KEVLTAALFS KPELLNKALA GEAVSLKLVS
310 320 330 340 350
VGLLTASNIF GKEGTMVEDQ MRAWQSLTQP GKMIHLKIRN KDGDLQTVKI
360 370 380 390 400
KPDVAAFNVG VNELALKLGF GLKASDSYNA EALHQLLGND LRPEARPGGW
410 420 430 440 450
VGEWLAQYPD NYEVVNTLAR QIKDIWKNNQ HHKDGGEPYK LAQRLAMLAH
460 470 480 490 500
EIDAVPAWNC KSGKDRTGMM DSEIKREIIS LHQTHMLSAP GSLPDSGGQK
510 520 530 540 550
IFQKVLLNSG NLEIQKQNTG GAGNKVMKNL SPEVLNLSYQ KRVGDENIWQ
560
SVKGISSLIT S
Length:561
Mass (Da):61,935
Last modified:October 11, 2004 - v2
Checksum:iA484B55502CFA643
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti244 – 2441C → FD in AAC46234 (PubMed:9537377).Curated
Sequence conflicti354 – 3541V → VV in AAC46234 (PubMed:9537377).Curated
Sequence conflicti476 – 4761R → G in AAC46234 (PubMed:9537377).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF021817 Genomic DNA. Translation: AAC46234.1.
AE006468 Genomic DNA. Translation: AAL20023.1.
AF213335 Genomic DNA. Translation: AAF21057.2.
RefSeqiNP_460064.1. NC_003197.1.
WP_001166946.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20023; AAL20023; STM1091.
GeneIDi1252609.
KEGGistm:STM1091.
PATRICi32380619. VBISalEnt20916_1155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF021817 Genomic DNA. Translation: AAC46234.1.
AE006468 Genomic DNA. Translation: AAL20023.1.
AF213335 Genomic DNA. Translation: AAF21057.2.
RefSeqiNP_460064.1. NC_003197.1.
WP_001166946.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DIDX-ray2.35B30-181[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO30916. 1 interaction.
MINTiMINT-157109.
STRINGi99287.STM1091.

Proteomic databases

PaxDbiO30916.
PRIDEiO30916.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20023; AAL20023; STM1091.
GeneIDi1252609.
KEGGistm:STM1091.
PATRICi32380619. VBISalEnt20916_1155.

Phylogenomic databases

eggNOGiENOG4108N4M. Bacteria.
ENOG410Y7WM. LUCA.
HOGENOMiHOG000028303.
KOiK13085.
OMAiCWNCKSG.
OrthoDBiEOG67DPGJ.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15186.
SENT99287:GCTI-1100-MONOMER.
BRENDAi3.1.3.78. 5542.

Family and domain databases

InterProiIPR008108. IpgD/SopB.
[Graphical view]
PfamiPF05925. IpgD. 1 hit.
[Graphical view]
PRINTSiPR01734. TYPE3OMBPROT.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel Salmonella invasion locus homologous to Shigella ipgDE."
    Hong K.H., Miller V.L.
    J. Bacteriol. 180:1793-1802(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 14028s / SGSG 2262.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  3. "Prevalence and polymorphism of genes encoding translocated effector proteins among clinical isolates of Salmonella enterica."
    Prager R., Mirold S., Tietze E., Strutz U., Knuppel B., Rabsch W., Hardt W.-D., Tschape H.
    Int. J. Med. Microbiol. 290:605-617(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 97-529.
    Strain: IE1534.
  4. "Salmonella host cell invasion emerged by acquisition of a mosaic of separate genetic elements, including Salmonella pathogenicity island 1 (SPI1), SPI5, and sopE2."
    Mirold S., Ehrbar K., Weissmueller A., Prager R., Tschaepe H., Ruessmann H., Hardt W.-D.
    J. Bacteriol. 183:2348-2358(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN HOST CELL INVASION, SUBCELLULAR LOCATION.
    Strain: SL1344.
  5. "A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host cell actin cytoskeleton rearrangements and bacterial internalization."
    Zhou D., Chen L.-M., Hernandez L.D., Shears S.B., Galan J.E.
    Mol. Microbiol. 39:248-259(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Elimination of host cell PtdIns(4,5)P(2) by bacterial SigD promotes membrane fission during invasion by Salmonella."
    Terebiznik M.R., Vieira O.V., Marcus S.L., Slade A., Yip C.M., Trimble W.S., Meyer T., Finlay B.B., Grinstein S.
    Nat. Cell Biol. 4:766-773(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: SLI344.
  7. "Salmonella modulates vesicular traffic by altering phosphoinositide metabolism."
    Hernandez L.D., Hueffer K., Wenk M.R., Galan J.E.
    Science 304:1805-1807(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "A synaptojanin-homologous region of Salmonella typhimurium SigD is essential for inositol phosphatase activity and Akt activation."
    Marcus S.L., Wenk M.R., Steele-Mortimer O., Finlay B.B.
    FEBS Lett. 494:201-207(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF CYS-460; LYS-525 AND LYS-528.

Entry informationi

Entry nameiSOPB_SALTY
AccessioniPrimary (citable) accession number: O30916
Secondary accession number(s): Q8ZQ57, Q9L889
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 11, 2015
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.