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Protein

Inositol phosphate phosphatase SopB

Gene

sopB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns 3-P and prevents the transition of PtdIns 3-P to PtdIns 3,5-P2. It is one of the known effectors injected by Salmonella into the host cell and is required for invasion and for an efficient generation and maintenance of Salmonella-containing vacuole (SVC). Alteration of the phosphoinositide composition of the plasma membrane causes membrane ruffling and actin cytoskeleton rearrangements. The persistence of PtdIns 3-P diverts the SCV from the endocytic pathway resulting in enlarged vesicles, which are essential to create a favorable environment where Salmonella can replicate and avoid immune defenses of the host cell.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei460Sequence analysis1

GO - Molecular functioni

  • lipid phosphatase activity Source: AgBase

GO - Biological processi

  • negative regulation of cell growth Source: AgBase
  • negative regulation of signaling Source: AgBase
  • pathogenesis Source: UniProtKB-KW
  • positive regulation of DNA replication Source: AgBase
  • regulation of protein kinase activity Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15186.
BRENDAi3.1.3.78. 5542.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol phosphate phosphatase SopB (EC:3.1.3.-)
Alternative name(s):
Effector protein SopB
Gene namesi
Name:sopB
Synonyms:sigD
Ordered Locus Names:STM1091
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Secreted By similarity

  • Note: Secreted via the type III secretion system 1 (SPI-1 TTSS).By similarity

GO - Cellular componenti

  • cell periphery Source: AgBase
  • symbiont-containing vacuole Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi460C → S: Loss of activity. 1 Publication1
Mutagenesisi525K → A: Decrease in activity. 1 Publication1
Mutagenesisi528K → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002204951 – 561Inositol phosphate phosphatase SopBAdd BLAST561

Proteomic databases

PaxDbiO30916.
PRIDEiO30916.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC42P609535EBI-11167349,EBI-81752From a different organism.

Protein-protein interaction databases

IntActiO30916. 1 interactor.
MINTiMINT-157109.
STRINGi99287.STM1091.

Structurei

Secondary structure

1561
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi49 – 52Combined sources4
Helixi59 – 62Combined sources4
Beta strandi69 – 72Combined sources4
Helixi73 – 90Combined sources18
Turni91 – 93Combined sources3
Helixi96 – 107Combined sources12
Helixi108 – 110Combined sources3
Helixi118 – 142Combined sources25
Helixi147 – 166Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DIDX-ray2.35B30-181[»]
SMRiO30916.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi460 – 466CX5R motif7

Domaini

Contains the consensus sequence Cys-X(5)-Arg characteristic of Mg-independent phosphatases.

Sequence similaritiesi

Belongs to the phosphatase IpgD/SopB family.Curated

Phylogenomic databases

eggNOGiENOG4108N4M. Bacteria.
ENOG410Y7WM. LUCA.
HOGENOMiHOG000028303.
KOiK13085.
OMAiCWNCKSG.

Family and domain databases

InterProiIPR008108. IpgD/SopB.
[Graphical view]
PfamiPF05925. IpgD. 1 hit.
[Graphical view]
PRINTSiPR01734. TYPE3OMBPROT.

Sequencei

Sequence statusi: Complete.

O30916-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIQSFYHSA SLKTQEAFKS LQKTLYNGMQ ILSGQGKAPA KAPDARPEII
60 70 80 90 100
VLREPGATWG NYLQHQKASN HSLHNLYNLQ RDLLTVAATV LGKQDPVLTS
110 120 130 140 150
MANQMELAKV KADRPATKQE EAAAKALKKN LIELIAARTQ QQDGLPAKEA
160 170 180 190 200
HRFAAVAFRD AQVKQLNNQP WQTIKNTLTH NGHHYTNTQL PAAEMKIGAK
210 220 230 240 250
DIFPSAYEGK GVCSWDTKNI HHANNLWMST VSVHEDGKDK TLFCGIRHGV
260 270 280 290 300
LSPYHEKDPL LRHVGAENKA KEVLTAALFS KPELLNKALA GEAVSLKLVS
310 320 330 340 350
VGLLTASNIF GKEGTMVEDQ MRAWQSLTQP GKMIHLKIRN KDGDLQTVKI
360 370 380 390 400
KPDVAAFNVG VNELALKLGF GLKASDSYNA EALHQLLGND LRPEARPGGW
410 420 430 440 450
VGEWLAQYPD NYEVVNTLAR QIKDIWKNNQ HHKDGGEPYK LAQRLAMLAH
460 470 480 490 500
EIDAVPAWNC KSGKDRTGMM DSEIKREIIS LHQTHMLSAP GSLPDSGGQK
510 520 530 540 550
IFQKVLLNSG NLEIQKQNTG GAGNKVMKNL SPEVLNLSYQ KRVGDENIWQ
560
SVKGISSLIT S
Length:561
Mass (Da):61,935
Last modified:October 11, 2004 - v2
Checksum:iA484B55502CFA643
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti244C → FD in AAC46234 (PubMed:9537377).Curated1
Sequence conflicti354V → VV in AAC46234 (PubMed:9537377).Curated1
Sequence conflicti476R → G in AAC46234 (PubMed:9537377).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF021817 Genomic DNA. Translation: AAC46234.1.
AE006468 Genomic DNA. Translation: AAL20023.1.
AF213335 Genomic DNA. Translation: AAF21057.2.
RefSeqiNP_460064.1. NC_003197.1.
WP_001166946.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20023; AAL20023; STM1091.
GeneIDi1252609.
KEGGistm:STM1091.
PATRICi32380619. VBISalEnt20916_1155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF021817 Genomic DNA. Translation: AAC46234.1.
AE006468 Genomic DNA. Translation: AAL20023.1.
AF213335 Genomic DNA. Translation: AAF21057.2.
RefSeqiNP_460064.1. NC_003197.1.
WP_001166946.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DIDX-ray2.35B30-181[»]
SMRiO30916.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO30916. 1 interactor.
MINTiMINT-157109.
STRINGi99287.STM1091.

Proteomic databases

PaxDbiO30916.
PRIDEiO30916.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20023; AAL20023; STM1091.
GeneIDi1252609.
KEGGistm:STM1091.
PATRICi32380619. VBISalEnt20916_1155.

Phylogenomic databases

eggNOGiENOG4108N4M. Bacteria.
ENOG410Y7WM. LUCA.
HOGENOMiHOG000028303.
KOiK13085.
OMAiCWNCKSG.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15186.
BRENDAi3.1.3.78. 5542.

Family and domain databases

InterProiIPR008108. IpgD/SopB.
[Graphical view]
PfamiPF05925. IpgD. 1 hit.
[Graphical view]
PRINTSiPR01734. TYPE3OMBPROT.
ProtoNetiSearch...

Entry informationi

Entry nameiSOPB_SALTY
AccessioniPrimary (citable) accession number: O30916
Secondary accession number(s): Q8ZQ57, Q9L889
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.