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O30869 (T2H2_HAEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme HaeII

Short name=R.HaeII
EC=3.1.21.4
Alternative name(s):
Endonuclease HaeII
Type II restriction enzyme HaeII
Gene names
Name:haeIIR
Synonyms:dcrA
OrganismHaemophilus aegyptius
Taxonomic identifier197575 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length352 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence RGCGCY and cleaves after C-5.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 352352Type-2 restriction enzyme HaeII
PRO_0000077316

Sequences

Sequence LengthMass (Da)Tools
O30869 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 3AE01F59FB1C289A

FASTA35240,801
        10         20         30         40         50         60 
MNDIQVAKES LDKIIKKARV HLYKPIQIAE ILYQDRVNKN INLLDKETYR NISKKWRDVI 

        70         80         90        100        110        120 
CIRFLGRVST SSAKYQDDLF NDNAMPPKHL NILGEMNRKT NGGVESYIYK KFFERFNQMS 

       130        140        150        160        170        180 
SALEYSYSRT PDNFHLSEFL ALFWLEPGLK RSIDKVYEIV VYALFSSLIE ALGVKVKIDL 

       190        200        210        220        230        240 
DLSNIDLLKE FEDFTRQIIS LDSENTSLEL NAKINRVGVT NASDRGLDMW ANFGMAIQIK 

       250        260        270        280        290        300 
HLSLTEELAE NIVSSVSSDR IVIVCKESEE KLILSLLNQI GWRSKIQSII TEADLIKWYD 

       310        320        330        340        350 
KALRGKSAYL VGSKILEHIR NEINLEFPAT DIVDFNHFFH EREYNKALDI EK 

« Hide

References

[1]"Sequence similarities between the genes encoding the S.NgoI and HaeII restriction/modification systems."
Stein D.C., Gunn J.S., Piekarowicz A.
Biol. Chem. 379:575-578(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 11116 / CCUG 25716 / NCTC 8502.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF019752 Genomic DNA. Translation: AAB70830.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1088. HaeII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019058. Restrct_endonuc_II_HaeII.
[Graphical view]
PfamPF09554. RE_HaeII. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2H2_HAEAE
AccessionPrimary (citable) accession number: O30869
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries