O30869 (T2H2_HAEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme HaeII
Short name=R.HaeII
EC=3.1.21.4

Alternative name(s):
Endonuclease HaeII
Type II restriction enzyme HaeII

Gene names

Name:	haeIIR
Synonyms:	dcrA

Organism

Haemophilus aegyptius

Taxonomic identifier

197575 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence RGCGCY and cleaves after C-5.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Type-2 restriction enzyme HaeII		PRO_0000077316

Sequences

	Sequence		Length	Mass (Da)
	O30869 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 3AE01F59FB1C289A	FASTA	352	40,801
	10 20 30 40 50 60 MNDIQVAKES LDKIIKKARV HLYKPIQIAE ILYQDRVNKN INLLDKETYR NISKKWRDVI 70 80 90 100 110 120 CIRFLGRVST SSAKYQDDLF NDNAMPPKHL NILGEMNRKT NGGVESYIYK KFFERFNQMS 130 140 150 160 170 180 SALEYSYSRT PDNFHLSEFL ALFWLEPGLK RSIDKVYEIV VYALFSSLIE ALGVKVKIDL 190 200 210 220 230 240 DLSNIDLLKE FEDFTRQIIS LDSENTSLEL NAKINRVGVT NASDRGLDMW ANFGMAIQIK 250 260 270 280 290 300 HLSLTEELAE NIVSSVSSDR IVIVCKESEE KLILSLLNQI GWRSKIQSII TEADLIKWYD 310 320 330 340 350 KALRGKSAYL VGSKILEHIR NEINLEFPAT DIVDFNHFFH EREYNKALDI EK

References

[1]	"Sequence similarities between the genes encoding the S.NgoI and HaeII restriction/modification systems." Stein D.C., Gunn J.S., Piekarowicz A. Biol. Chem. 379:575-578(1998) [PubMed: 9628358] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 11116 / CCUG 25716 / NCTC 8502.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF019752 Genomic DNA. Translation: AAB70830.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1088. HaeII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019058. Restrct_endonuc_II_HaeII. [Graphical view]
Pfam	PF09554. RE_HaeII. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2H2_HAEAE

Accession

Primary (citable) accession number: O30869

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 1, 1998
Last modified:	May 31, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program