Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O30868 (MTH2_HAEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase HaeII

Short name=M.HaeII
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase HaeII
Gene names
Name:haeIIM
Synonyms:dcmA
OrganismHaemophilus aegyptius
Taxonomic identifier197575 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence RGCGCY, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HaeII endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Modification methylase HaeII
PRO_0000087877

Regions

Domain4 – 304301SAM-dependent MTase C5-type

Sites

Active site731 By similarity

Sequences

Sequence LengthMass (Da)Tools
O30868 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: CA879CF66731D434

FASTA31835,670
        10         20         30         40         50         60 
MKEYKTIDLF AGIGGIRLGF EAFGCKNVFS SEWDKYAQSM YEVNFGEKPF GDINDISPSD 

        70         80         90        100        110        120 
IPDHDILLAG FPCQPFSIAG KGLGFADTRG TLFFNIEAIL KAKKPKAFLL ENVKRLTTHD 

       130        140        150        160        170        180 
NGNTFKVIND KLNKLGYTVY HKVLNTLDFG LPQKRERIYI VGFLDKLHFE FPKPIGKYEE 

       190        200        210        220        230        240 
LSNILESDDV VPNNYFLSDE LKRKRLSSIT KDVPYPSIWH ENISGNVSAL PYSCALSVGG 

       250        260        270        280        290        300 
SYNYLVVNGV RRLTGPEMLR LQGFPDTFEI NIPYSQVRKV AGNSVSVPVI RAIAGSMINS 

       310 
LNMATRKIYQ AIQLSLLD 

« Hide

References

[1]"Sequence similarities between the genes encoding the S.NgoI and HaeII restriction/modification systems."
Stein D.C., Gunn J.S., Piekarowicz A.
Biol. Chem. 379:575-578(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 11116 / CCUG 25716 / NCTC 8502.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF019752 Genomic DNA. Translation: AAB70829.1.

3D structure databases

ProteinModelPortalO30868.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3409. M.HaeII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTH2_HAEAE
AccessionPrimary (citable) accession number: O30868
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: October 16, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries