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Protein

Modification methylase HaeII

Gene

haeIIM

Organism
Haemophilus aegyptius
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence RGCGCY, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HaeII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3409. M.HaeII.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HaeII (EC:2.1.1.37)
Short name:
M.HaeII
Alternative name(s):
Cytosine-specific methyltransferase HaeII
Gene namesi
Name:haeIIM
Synonyms:dcmA
OrganismiHaemophilus aegyptius
Taxonomic identifieri197575 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878771 – 318Modification methylase HaeIIAdd BLAST318

Structurei

3D structure databases

ProteinModelPortaliO30868.
SMRiO30868.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 304SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST301

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiView protein in InterPro
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR031303. C5_meth_CS.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF00145. DNA_methylase. 1 hit.
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiView protein in PROSITE
PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.

Sequencei

Sequence statusi: Complete.

O30868-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEYKTIDLF AGIGGIRLGF EAFGCKNVFS SEWDKYAQSM YEVNFGEKPF
60 70 80 90 100
GDINDISPSD IPDHDILLAG FPCQPFSIAG KGLGFADTRG TLFFNIEAIL
110 120 130 140 150
KAKKPKAFLL ENVKRLTTHD NGNTFKVIND KLNKLGYTVY HKVLNTLDFG
160 170 180 190 200
LPQKRERIYI VGFLDKLHFE FPKPIGKYEE LSNILESDDV VPNNYFLSDE
210 220 230 240 250
LKRKRLSSIT KDVPYPSIWH ENISGNVSAL PYSCALSVGG SYNYLVVNGV
260 270 280 290 300
RRLTGPEMLR LQGFPDTFEI NIPYSQVRKV AGNSVSVPVI RAIAGSMINS
310
LNMATRKIYQ AIQLSLLD
Length:318
Mass (Da):35,670
Last modified:January 1, 1998 - v1
Checksum:iCA879CF66731D434
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019752 Genomic DNA. Translation: AAB70829.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019752 Genomic DNA. Translation: AAB70829.1.

3D structure databases

ProteinModelPortaliO30868.
SMRiO30868.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3409. M.HaeII.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiView protein in InterPro
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR031303. C5_meth_CS.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF00145. DNA_methylase. 1 hit.
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiView protein in PROSITE
PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTH2_HAEAE
AccessioniPrimary (citable) accession number: O30868
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: February 15, 2017
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.