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Protein

Modification methylase HaeII

Gene

haeIIM

Organism
Haemophilus aegyptius
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence RGCGCY, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HaeII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3409. M.HaeII.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HaeII (EC:2.1.1.37)
Short name:
M.HaeII
Alternative name(s):
Cytosine-specific methyltransferase HaeII
Gene namesi
Name:haeIIM
Synonyms:dcmA
OrganismiHaemophilus aegyptius
Taxonomic identifieri197575 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Modification methylase HaeIIPRO_0000087877Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO30868.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 304301SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O30868-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKEYKTIDLF AGIGGIRLGF EAFGCKNVFS SEWDKYAQSM YEVNFGEKPF
60 70 80 90 100
GDINDISPSD IPDHDILLAG FPCQPFSIAG KGLGFADTRG TLFFNIEAIL
110 120 130 140 150
KAKKPKAFLL ENVKRLTTHD NGNTFKVIND KLNKLGYTVY HKVLNTLDFG
160 170 180 190 200
LPQKRERIYI VGFLDKLHFE FPKPIGKYEE LSNILESDDV VPNNYFLSDE
210 220 230 240 250
LKRKRLSSIT KDVPYPSIWH ENISGNVSAL PYSCALSVGG SYNYLVVNGV
260 270 280 290 300
RRLTGPEMLR LQGFPDTFEI NIPYSQVRKV AGNSVSVPVI RAIAGSMINS
310
LNMATRKIYQ AIQLSLLD
Length:318
Mass (Da):35,670
Last modified:January 1, 1998 - v1
Checksum:iCA879CF66731D434
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019752 Genomic DNA. Translation: AAB70829.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019752 Genomic DNA. Translation: AAB70829.1.

3D structure databases

ProteinModelPortaliO30868.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3409. M.HaeII.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence similarities between the genes encoding the S.NgoI and HaeII restriction/modification systems."
    Stein D.C., Gunn J.S., Piekarowicz A.
    Biol. Chem. 379:575-578(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 11116 / CCUG 25716 / NCTC 8502.

Entry informationi

Entry nameiMTH2_HAEAE
AccessioniPrimary (citable) accession number: O30868
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: January 7, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.