O30853 (KPYK1_PHOLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate kinase I
EC=2.7.1.40

Alternative name(s):
PK-1

Gene names

Name:

pki

Organism

Photobacterium leiognathi

Taxonomic identifier

553611 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	235 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Potassium.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 235	›235	Pyruvate kinase I		PRO_0000112083
Sites
	Metal binding	11	1	Magnesium By similarity
	Binding site	10	1	Substrate; via amide nitrogen By similarity
	Binding site	11	1	Substrate; via amide nitrogen By similarity
	Binding site	43	1	Substrate By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	O30853 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: F805606C4C8EF4C1	FASTA	235	24,734
	10 20 30 40 50 60 ASDGIMVARG DLGVEIPVEE VIFAQKMMIE KCNRARKVVI TATQMLDSMI KNPRPTRAEA 70 80 90 100 110 120 GDVANAIMDG TDAVMLSGES AKGKYPIEAV TIMAQICART DDVVKPELGS RLDSPRLRIT 130 140 150 160 170 180 EAVCKGAVDT AEKLNAPLII VATEAGKSAR SVRKYFPTAR ILAVTTNTKT AAQLCLSKGV 190 200 210 220 230 TPVVVDSIES TDAFYLRGKE LALETGLGAK GDIVVMVSGA LVASGTTNTA SVHVL

References

[1]

"The pkI gene encoding pyruvate kinase I links to the luxZ gene which enhances bioluminescence of the lux operon from Photobacterium leiognathi." Lin J.-W., Lu H.-C., Chen H.-Y., Weng S.-F. Biochem. Biophys. Res. Commun. 239:228-234(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25521 / L1 / CIP 665.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF019143 Genomic DNA. Translation: AAC45776.1.
PIR	PC4418.
3D structure databases
ProteinModelPortal	O30853.
SMR	O30853. Positions 1-235.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00109; UER00188.
Family and domain databases
Gene3D	3.20.20.60. 1 hit. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase. IPR015794. Pyrv_Knase_a/b. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK1_PHOLE

Accession

Primary (citable) accession number: O30853

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program