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Protein

NADP-dependent malic enzyme

Gene

tme

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+, Mn2+Note: Divalent metal cations. Prefers magnesium or manganese.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei104Proton donorBy similarity1
Metal bindingi146Divalent metal cationBy similarity1
Metal bindingi147Divalent metal cationBy similarity1
Active sitei172Proton acceptorBy similarity1
Binding sitei172NADBy similarity1
Binding sitei297NADBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMultifunctional enzyme, Oxidoreductase
LigandMetal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-dependent malic enzyme (EC:1.1.1.40)
Short name:
NADP-ME
Gene namesi
Name:tme
Ordered Locus Names:R00394
ORF Names:SMc01126
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001602461 – 761NADP-dependent malic enzymeAdd BLAST761

Interactioni

Subunit structurei

Homooctamer.

Protein-protein interaction databases

STRINGi266834.SMc01126

Structurei

3D structure databases

ProteinModelPortaliO30808
SMRiO30808
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 437Malic enzymeAdd BLAST437
Regioni438 – 761Phosphate acetyltransferaseAdd BLAST324

Sequence similaritiesi

In the N-terminal section; belongs to the malic enzymes family.Curated
In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.Curated

Phylogenomic databases

eggNOGiENOG4105C6K Bacteria
COG0280 LUCA
COG0281 LUCA
HOGENOMiHOG000132448
KOiK00029
OMAiINCVRFL

Family and domain databases

Gene3Di3.40.50.10380, 1 hit
InterProiView protein in InterPro
IPR015884 Malic_enzyme_CS
IPR012301 Malic_N_dom
IPR037062 Malic_N_dom_sf
IPR012302 Malic_NAD-bd
IPR012188 ME_PTA
IPR036291 NAD(P)-bd_dom_sf
IPR002505 PTA_PTB
PfamiView protein in Pfam
PF00390 malic, 1 hit
PF03949 Malic_M, 1 hit
PF01515 PTA_PTB, 1 hit
PIRSFiPIRSF036684 ME_PTA, 1 hit
SMARTiView protein in SMART
SM01274 malic, 1 hit
SM00919 Malic_M, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00331 MALIC_ENZYMES, 1 hit

Sequencei

Sequence statusi: Complete.

O30808-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGIDKTDRA MTSVTAQEAL DFHSQGRPGK LEISPTKPMA TQRDLSLAYS
60 70 80 90 100
PGVAVPVKAI ADDPATAYDY TARGNMVAVI SNGTAILGLG NLGALASKPV
110 120 130 140 150
MEGKAVLFKR FADVDSIDLE VDTENVDEFV NCVRFLGPSF GGINLEDIKA
160 170 180 190 200
PDCFIIEQRL REVMDIPVFH DDQHGTAIIA AAGLVNALTL TGRDFKTAKL
210 220 230 240 250
VCNGAGAAAI ACIELIKAMG FNPENIILCD TKGVIYKGRT DGMNQWKSAH
260 270 280 290 300
AVETDRRTLA EALDGADVFF GLSAKGALSA DMVRSMGARP IIFAMANPDP
310 320 330 340 350
EITPEEVALI RDDAIVATGR SDYPNQVNNV LGFPYIFRGA LDVRASTIND
360 370 380 390 400
AMKIAAAEAL ANLAKEDVPD DVAAAYQGNR PRFGPQYIIP VPFDPRLISA
410 420 430 440 450
IPMAVAKAAM ETGVARKPIE DLKAYGQQLS ARRDPIASTL QRIVERVRRQ
460 470 480 490 500
PKRIVFAEGE EVQMMRSAIA YANQQLGTAL LLGREEVMRE TAEREGIDLD
510 520 530 540 550
RAGIQIVNAR LSKRVGAYTD FLYSRLQRKG YLFRDVQRLI NTDRNHFAAS
560 570 580 590 600
MVALGDADGM VTGLTRNYST ALEDVRRCID PKPGHRVIGV SIALCRGRTV
610 620 630 640 650
LVADTAVHDM PTSEELADIA EEAAGLAKRL GYVPRVAMLA YSTFGHPSGE
660 670 680 690 700
RSERVREAVK ILDRRRVDFE YDGEMAADVA LNARVMEQYP FCRLSGTANV
710 720 730 740 750
LVMPAFHSAS ISTKMLQELG GSTVIGPLLV GLDKSVQIAS MSAKDSDLVN
760
LAAIAAYNAG T
Length:761
Mass (Da):82,209
Last modified:May 30, 2000 - v2
Checksum:iA342A5F41F39DBC7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti228L → F in AAB82460 (PubMed:9535928).Curated1
Sequence conflicti609 – 636DMPTS…YVPRV → KHADLRGAG in AAB82460 (PubMed:9535928).CuratedAdd BLAST28
Sequence conflicti761T → TRRGSAESRKTAGTSSVAAS PDG in AAB82460 (PubMed:9535928).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017444 Genomic DNA Translation: AAB82460.1
AL591688 Genomic DNA Translation: CAC41831.1
RefSeqiNP_384500.1, NC_003047.1
WP_010968547.1, NC_003047.1

Genome annotation databases

EnsemblBacteriaiCAC41831; CAC41831; SMc01126
GeneIDi1232028
KEGGisme:SMc01126
PATRICifig|266834.11.peg.1767

Similar proteinsi

Entry informationi

Entry nameiMAO2_RHIME
AccessioniPrimary (citable) accession number: O30808
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: May 23, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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