Reviewed, UniProtKB/Swiss-Prot O30746 (ALR2_KLEAE)

Names and origin

Protein names

Recommended name:
    Alanine racemase, catabolic
    EC=5.1.1.1

Gene names

Name:

dadB

Organism

Klebsiella aerogenes

Taxonomic identifier

28451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by dadA. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201
Induction	By alanine. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Alanine racemase, catabolic HAMAP MF_01201		PRO_0000114525
Sites
	Active site	35	1	Proton acceptor; specific for D-alanine By similarity
	Active site	253	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	35	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O30746-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 45A7CD7E56855856	FASTA	356	38,788
	10 20 30 40 50 60 MTRPVVASID LLALRQNLQI VRRAAPGSRL WAVDKDNAYG HGVARVWSAL SAADGFALLN 70 80 90 100 110 120 LEEAILLREQ GWKGPILLLE GFFHADELAV LDQYRLPTSV HSNWQIKALQ QAKLRAPLDI 130 140 150 160 170 180 YLKVNSGMNR LGFMPERVHT VWQQLRAISN VGEMTLMSHF AEAENPQGIV EPMRRIEQAA 190 200 210 220 230 240 EGLDCPRSLA NSAATLWHPE AHFDWVRPGI VLYGASPSGQ WQDIANTGLK PVMTLRSEII 250 260 270 280 290 300 GVQNLRPGEA IGYGGLYRTT QEQRIGIVAC GYADGYPRVA PSGTPVLVDG VRTTTVGRVS 310 320 330 340 350 MDMLAVDLTP CPQAGIGAPV ELWGKEIKID DVAASSGTVG YELMCALAPR VPVVTL

References

[1]

"Alanine catabolism in Klebsiella aerogenes: molecular characterization of the dadAB operon and its regulation by the nitrogen assimilation control protein." Janes B.K., Bender R.A. J. Bacteriol. 180:563-570(1998) [PubMed: 9457858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: W70.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF016253 Genomic DNA. Translation: AAC38140.1.
3D structure databases
SMR	O30746. Positions 3-354.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.1.1.1. 366.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR2_KLEAE

Accession

Primary (citable) accession number: O30746

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)