Reviewed, UniProtKB/Swiss-Prot O30745 (DADA_KLEAE)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1

Gene names

Name:

dadA

Organism

Klebsiella aerogenes

Taxonomic identifier

28451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids. HAMAP MF_01202
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Induction	By alanine; slightly repressed by glucose. HAMAP MF_01202
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_0000166134
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	O30745-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 096986F47BDB4DD6	FASTA	432	47,251
	10 20 30 40 50 60 MRVVILGSGV FGVASAWYLS QAGHDVTVID RQPGPAEETS AANAGQISPG YAAPWAAPGV 70 80 90 100 110 120 PLKAIKWMFQ RHAPLAIGLD GTSFQLKWMW QMLRNCDTRH YMENKGRMVR LAEYSRDCLK 130 140 150 160 170 180 ALRDTTGIQY EGRQGGTLQL FRTAKQYENA TRDIAVLEDA GVPYQLLEAK RLAEVEPALA 190 200 210 220 230 240 EVSHKLTGGL RLPNDETGDC QLFTTRLAAM ADQAGVTFRF NTAVDALLHE GDRIAGVKCG 250 260 270 280 290 300 MRIIKGDAYV MAFGSYSTAM LKGLVDIPVY PLKGYSLTIP IAQEDGAPVS TILDVTYTIA 310 320 330 340 350 360 ITRFDQRIRV GGMAEIVGFN KTLLQPRRET LEMVVRDLFP RGGHVEQATF WTGLRPMTPD 370 380 390 400 410 420 GTPVVGRTAY KNLWLNTGHG TLGWTMACGS GQLISDLISG RTPAIPYDDL AVARYSPGFT 430 PARPQHLHGA HN

References

[1]

"Alanine catabolism in Klebsiella aerogenes: molecular characterization of the dadAB operon and its regulation by the nitrogen assimilation control protein." Janes B.K., Bender R.A. J. Bacteriol. 180:563-570(1998) [PubMed: 9457858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: W70.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF016253 Genomic DNA. Translation: AAC38139.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.99.1. 366.
Family and domain databases
HAMAP	MF_01202. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

DADA_KLEAE

Accession

Primary (citable) accession number: O30745

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	January 1, 1998
Last modified:	January 20, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)