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Protein

Monomethylamine methyltransferase MtmB1

Gene

mtmB1

Organism
Methanosarcina barkeri
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the methyl group from monomethylamine to the corrinoid cofactor of MtmC (MtmC1 or MtmC2).2 Publications

Catalytic activityi

Methylamine + a [Co(I) methylamine-specific corrinoid protein] = a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia.1 Publication

Pathwayi: methanogenesis from methylamine

This protein is involved in the pathway methanogenesis from methylamine, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway methanogenesis from methylamine and in One-carbon metabolism.

GO - Molecular functioni

  • monomethylamine methyltransferase activity Source: MENGO

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Methanogenesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12210.
BRENDAi2.1.1.248. 3250.
2.1.1.249. 3250.
UniPathwayiUPA00643.

Names & Taxonomyi

Protein namesi
Recommended name:
Monomethylamine methyltransferase MtmB1 (EC:2.1.1.248)
Short name:
MMA methyltransferase 1
Short name:
MMAMT 1
Gene namesi
Name:mtmB1
Synonyms:mtmB
OrganismiMethanosarcina barkeri
Taxonomic identifieri2208 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002165502 – 458Monomethylamine methyltransferase MtmB1Add BLAST457

Interactioni

Subunit structurei

Dimer of homotrimers. Can form a complex with MtmC (MtmC1 or MtmC2).

Protein-protein interaction databases

STRINGi269797.Mbar_A0843.

Structurei

Secondary structure

1458
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 18Combined sources10
Beta strandi19 – 21Combined sources3
Helixi24 – 28Combined sources5
Helixi31 – 42Combined sources12
Helixi56 – 73Combined sources18
Beta strandi75 – 77Combined sources3
Turni78 – 81Combined sources4
Beta strandi82 – 84Combined sources3
Helixi88 – 95Combined sources8
Beta strandi101 – 105Combined sources5
Helixi107 – 109Combined sources3
Beta strandi111 – 114Combined sources4
Beta strandi125 – 129Combined sources5
Helixi137 – 139Combined sources3
Helixi140 – 148Combined sources9
Beta strandi155 – 157Combined sources3
Beta strandi160 – 162Combined sources3
Helixi174 – 193Combined sources20
Helixi210 – 214Combined sources5
Beta strandi226 – 231Combined sources6
Turni234 – 236Combined sources3
Helixi240 – 251Combined sources12
Beta strandi255 – 259Combined sources5
Beta strandi266 – 268Combined sources3
Helixi271 – 288Combined sources18
Beta strandi292 – 296Combined sources5
Turni301 – 303Combined sources3
Helixi309 – 325Combined sources17
Beta strandi330 – 333Combined sources4
Beta strandi340 – 342Combined sources3
Helixi343 – 359Combined sources17
Beta strandi362 – 366Combined sources5
Helixi370 – 372Combined sources3
Helixi380 – 393Combined sources14
Helixi398 – 410Combined sources13
Turni411 – 414Combined sources4
Helixi415 – 417Combined sources3
Helixi425 – 428Combined sources4
Turni431 – 434Combined sources4
Helixi438 – 454Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1L2QX-ray1.70A1-458[»]
1NTHX-ray1.55A1-457[»]
1TV2X-ray2.00A1-457[»]
1TV3X-ray2.20A1-457[»]
1TV4X-ray1.80A1-457[»]
SMRiO30642.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO30642.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG05143. Archaea.
ENOG4110FS3. LUCA.

Family and domain databases

Gene3Di3.20.20.460. 1 hit.
InterProiIPR008031. MtmB_MeTrfase.
[Graphical view]
PfamiPF05369. MtmB. 1 hit.
[Graphical view]
SUPFAMiSSF75098. SSF75098. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O30642-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFRKSFDCY DFYDRAKVGE KCTQDDWDLM KIPMKAMELK QKYGLDFKGE
60 70 80 90 100
FIPTDKDMME KLFKAGFEML LECGIYCTDT HRIVKYTEDE IWDAINNVQK
110 120 130 140 150
EFVLGTGRDA VNVRKRSVGD KAKPIVQGGP TGSPISEDVF MPVHMSYALE
160 170 180 190 200
KEVDTIVNGV MTSVRGKSPI PKSPYEVLAA KTETRLIKNA CAMAGRPGMG
210 220 230 240 250
VOGPETSLSA QGNISADCTG GMTCTDSHEV SQLNELKIDL DAISVIAHYK
260 270 280 290 300
GNSDIIMDEQ MPIFGGYAGG IEETTIVDVA THINAVLMSS ASWHLDGPVH
310 320 330 340 350
IRWGSTNTRE TLMIAGWACA TISEFTDILS GNQYYPCAGP CTEMCLLEAS
360 370 380 390 400
AQSITDTASG REILSGVASA KGVVTDKTTG MEARMMGEVA RATAGVEISE
410 420 430 440 450
VNVILDKLVS LYEKNYASAP AGKTFQECYD VKTVTPTEEY MQVYDGARKK

LEDLGLVF
Length:458
Mass (Da):50,238
Last modified:February 26, 2008 - v6
Checksum:iCB75A20B1E5C0A2C
GO

Mass spectrometryi

Molecular mass is 50105±2 Da from positions 2 - 458. Determined by ESI. 1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti36A → S in strain: NIH. 1
Natural varianti219T → A in strain: NIH. 1

Non-standard residue

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-standard residuei202Pyrrolysine1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013713 Genomic DNA. Translation: AAC38636.3.
AF046875 Genomic DNA. Translation: AAC38637.1.

Keywords - Coding sequence diversityi

Pyrrolysine

Cross-referencesi

Web resourcesi

Protein Spotlight

Life's jokers - Issue 25 of August 2002

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013713 Genomic DNA. Translation: AAC38636.3.
AF046875 Genomic DNA. Translation: AAC38637.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1L2QX-ray1.70A1-458[»]
1NTHX-ray1.55A1-457[»]
1TV2X-ray2.00A1-457[»]
1TV3X-ray2.20A1-457[»]
1TV4X-ray1.80A1-457[»]
SMRiO30642.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269797.Mbar_A0843.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiarCOG05143. Archaea.
ENOG4110FS3. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00643.
BioCyciMetaCyc:MONOMER-12210.
BRENDAi2.1.1.248. 3250.
2.1.1.249. 3250.

Miscellaneous databases

EvolutionaryTraceiO30642.

Family and domain databases

Gene3Di3.20.20.460. 1 hit.
InterProiIPR008031. MtmB_MeTrfase.
[Graphical view]
PfamiPF05369. MtmB. 1 hit.
[Graphical view]
SUPFAMiSSF75098. SSF75098. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTMB1_METBA
AccessioniPrimary (citable) accession number: O30642
Secondary accession number(s): O52752
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: February 26, 2008
Last modified: November 2, 2016
This is version 85 of the entry and version 6 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

MtmB1 transcript is abundant relative to the mtmB2 transcript.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.