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O30601 (YOSP_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
SPBc2 prophage-derived ribonucleoside-diphosphate reductase subunit beta

EC=1.17.4.1
Alternative name(s):
Ribonucleotide reductase small subunit
Gene names
Name:yosP
Synonyms:yojQ/yojS
Ordered Locus Names:BSU20040
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

Binds 2 iron ions per subunit By similarity.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Tetramer of two alpha and two beta subunits By similarity.

Miscellaneous

This gene has an intron within which is encoded YosQ, a putative homing endonuclease.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase small chain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329SPBc2 prophage-derived ribonucleoside-diphosphate reductase subunit beta
PRO_0000389010

Sites

Active site1051 By similarity
Metal binding661Iron 1 By similarity
Metal binding971Iron 1 By similarity
Metal binding971Iron 2 By similarity
Metal binding1011Iron 1 By similarity
Metal binding1641Iron 2 By similarity
Metal binding1981Iron 2 By similarity
Metal binding2011Iron 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
O30601 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: EC36E9DFC12BD269

FASTA32938,181
        10         20         30         40         50         60 
MTKIYDAANW SKHEDDFTQM FYNQNVKQFW LPEEIALNGD LLTWKYLGKN EQDTYMKVLA 

        70         80         90        100        110        120 
GLTLLDTEQG NTGMPIVAEH VDGHQRKAVL NFMAMMENAV HAKSYSNIFL TLAPTEQINE 

       130        140        150        160        170        180 
VFEWVKNNRF LQKKARTIVS VYKTIKKNDE ISLFKGMVAS VFLESFLFYS GFYYPLYFYG 

       190        200        210        220        230        240 
QGKLMQSGEI INLIIRDEAI HGVYVGLLAQ EIYKKQTPQK QKELYAWALN LLQELYENEL 

       250        260        270        280        290        300 
EYTEDVYDQV GLAPDVKKFI RYNANKALNN LGFDHWFEEE DVNPIVINGL NTKTKSHDFF 

       310        320 
STKGNGYKKA TVEPLKDSDF IFTEKGCIQ 

« Hide

References

« Hide 'large scale' references
[1]"An 8 kb nucleotide sequence at the 3' flanking region of the sspC gene (184 degrees) on the Bacillus subtilis 168 chromosome containing an intein and an intron."
Ghim S.-Y., Choi S.-K., Shin B.-S., Park S.-H.
DNA Res. 5:121-126(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROPOSAL OF INTRON.
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO C-TERMINUS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF012906 Genomic DNA. Translation: AAB92486.1.
AL009126 Genomic DNA. Translation: CAB13896.2.
PIRB69927.
RefSeqNP_389886.2. NC_000964.3.

3D structure databases

ProteinModelPortalO30601.
SMRO30601. Positions 5-286.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU20040.

Proteomic databases

PaxDbO30601.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB13896; CAB13896; BSU20040.
GeneID939476.
KEGGbsu:BSU20040.
PATRIC18975853. VBIBacSub10457_2117.

Organism-specific databases

GenoListBSU20040.

Phylogenomic databases

eggNOGCOG0208.
HOGENOMHOG000246154.
KOK00526.
OMAHYEANEP.
OrthoDBEOG68DD19.
PhylomeDBO30601.

Enzyme and pathway databases

BioCycBSUB:BSU20040-MONOMER.
UniPathwayUPA00326.

Family and domain databases

Gene3D1.10.620.20. 1 hit.
InterProIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026494. RNR_1b_NrdF.
IPR026023. RNR_bsu_prok.
IPR000358. RNR_small.
[Graphical view]
PANTHERPTHR23409. PTHR23409. 1 hit.
PfamPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
PIRSFPIRSF000355. NrdB. 1 hit.
SUPFAMSSF47240. SSF47240. 1 hit.
TIGRFAMsTIGR04171. RNR_1b_NrdF. 1 hit.
PROSITEPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameYOSP_BACSU
AccessionPrimary (citable) accession number: O30601
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList