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O30601

- YOSP_BACSU

UniProt

O30601 - YOSP_BACSU

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Protein

SPBc2 prophage-derived ribonucleoside-diphosphate reductase subunit beta

Gene
yosP, yojQ/yojS, BSU20040
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

Binds 2 iron ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi66 – 661Iron 1 By similarity
Metal bindingi97 – 971Iron 1 By similarity
Metal bindingi97 – 971Iron 2 By similarity
Metal bindingi101 – 1011Iron 1 By similarity
Active sitei105 – 1051 By similarity
Metal bindingi164 – 1641Iron 2 By similarity
Metal bindingi198 – 1981Iron 2 By similarity
Metal bindingi201 – 2011Iron 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. deoxyribonucleotide biosynthetic process Source: InterPro
  3. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU20040-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
SPBc2 prophage-derived ribonucleoside-diphosphate reductase subunit beta (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
Name:yosP
Synonyms:yojQ/yojS
Ordered Locus Names:BSU20040
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU20040.

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329SPBc2 prophage-derived ribonucleoside-diphosphate reductase subunit betaPRO_0000389010Add
BLAST

Proteomic databases

PaxDbiO30601.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits By similarity.

Protein-protein interaction databases

STRINGi224308.BSU20040.

Structurei

3D structure databases

ProteinModelPortaliO30601.
SMRiO30601. Positions 5-286.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0208.
HOGENOMiHOG000246154.
KOiK00526.
OMAiHYEANEP.
OrthoDBiEOG68DD19.
PhylomeDBiO30601.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026023. RNR_bsu_prok.
IPR026494. RNR_NrdF.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
PIRSFiPIRSF000355. NrdB. 1 hit.
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR04171. RNR_1b_NrdF. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O30601-1 [UniParc]FASTAAdd to Basket

« Hide

MTKIYDAANW SKHEDDFTQM FYNQNVKQFW LPEEIALNGD LLTWKYLGKN    50
EQDTYMKVLA GLTLLDTEQG NTGMPIVAEH VDGHQRKAVL NFMAMMENAV 100
HAKSYSNIFL TLAPTEQINE VFEWVKNNRF LQKKARTIVS VYKTIKKNDE 150
ISLFKGMVAS VFLESFLFYS GFYYPLYFYG QGKLMQSGEI INLIIRDEAI 200
HGVYVGLLAQ EIYKKQTPQK QKELYAWALN LLQELYENEL EYTEDVYDQV 250
GLAPDVKKFI RYNANKALNN LGFDHWFEEE DVNPIVINGL NTKTKSHDFF 300
STKGNGYKKA TVEPLKDSDF IFTEKGCIQ 329
Length:329
Mass (Da):38,181
Last modified:January 1, 1998 - v1
Checksum:iEC36E9DFC12BD269
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012906 Genomic DNA. Translation: AAB92486.1.
AL009126 Genomic DNA. Translation: CAB13896.2.
PIRiB69927.
RefSeqiNP_389886.2. NC_000964.3.
WP_010886538.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB13896; CAB13896; BSU20040.
GeneIDi939476.
KEGGibsu:BSU20040.
PATRICi18975853. VBIBacSub10457_2117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012906 Genomic DNA. Translation: AAB92486.1 .
AL009126 Genomic DNA. Translation: CAB13896.2 .
PIRi B69927.
RefSeqi NP_389886.2. NC_000964.3.
WP_010886538.1. NC_000964.3.

3D structure databases

ProteinModelPortali O30601.
SMRi O30601. Positions 5-286.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU20040.

Proteomic databases

PaxDbi O30601.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB13896 ; CAB13896 ; BSU20040 .
GeneIDi 939476.
KEGGi bsu:BSU20040.
PATRICi 18975853. VBIBacSub10457_2117.

Organism-specific databases

GenoListi BSU20040.

Phylogenomic databases

eggNOGi COG0208.
HOGENOMi HOG000246154.
KOi K00526.
OMAi HYEANEP.
OrthoDBi EOG68DD19.
PhylomeDBi O30601.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci BSUB:BSU20040-MONOMER.

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026023. RNR_bsu_prok.
IPR026494. RNR_NrdF.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view ]
PIRSFi PIRSF000355. NrdB. 1 hit.
SUPFAMi SSF47240. SSF47240. 1 hit.
TIGRFAMsi TIGR04171. RNR_1b_NrdF. 1 hit.
PROSITEi PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An 8 kb nucleotide sequence at the 3' flanking region of the sspC gene (184 degrees) on the Bacillus subtilis 168 chromosome containing an intein and an intron."
    Ghim S.-Y., Choi S.-K., Shin B.-S., Park S.-H.
    DNA Res. 5:121-126(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROPOSAL OF INTRON.
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO C-TERMINUS.

Entry informationi

Entry nameiYOSP_BACSU
AccessioniPrimary (citable) accession number: O30601
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This gene has an intron within which is encoded YosQ, a putative homing endonuclease.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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