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O30569

- MTS1_RHIME

UniProt

O30569 - MTS1_RHIME

Protein

Modification methylase SmeI

Gene

smeIM

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSMEL266834:GJF6-946-MONOMER.

    Protein family/group databases

    REBASEi3264. M.SmeI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase SmeI (EC:2.1.1.72)
    Short name:
    M.SmeI
    Alternative name(s):
    Adenine-specific methyltransferase SmeIP
    M.CcrMI ortholog
    Modification methylase SmeIP
    Short name:
    M.SmeIP
    Gene namesi
    Name:smeIM
    Synonyms:ccrM
    Ordered Locus Names:R00926
    ORF Names:SMc00021
    OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
    Taxonomic identifieri266834 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001976: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 376376Modification methylase SmeIPRO_0000087988Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi266834.SMc00021.

    Structurei

    3D structure databases

    ProteinModelPortaliO30569.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0863.
    HOGENOMiHOG000125329.
    KOiK13581.
    OMAiFWHFEDG.
    OrthoDBiEOG6FV84T.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01555. N6_N4_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00508. S21N4MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O30569-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSVVSLAEI SRAARPLNWL DSIIKGDCVA ALNALPDHSV DVVFADPPYN    50
    LQLGGTLHRP DQSLVDAVDD DWDQFASFEA YDAFTRAWLL ACRRVLKPTG 100
    TLWVIGSYHN IFRVGAILQD LHFWVLNDII WRKTNPMPNF KGRRFQNAHE 150
    TLIWATPNAK AKGYTFNYEA MKAANDDVQM RSDWLFPICS GSERLKGDDG 200
    KKVHPTQKPE ALLARILMAS TKPGDVVLDP FFGSGTTGAV AKRLGRHFVG 250
    IEREQDYIDA AAERIAAVEP LGKATLSVMT GKKAEPRVAF NTLVESGLIK 300
    PGTVLTDAKR RYSAIVRADG TLASGGEAGS IHRLGAKVQG LDACNGWTFW 350
    HFEEGSVLKP IDELRSVIRN DLAKLN 376
    Length:376
    Mass (Da):41,442
    Last modified:May 30, 2000 - v2
    Checksum:i790DE7FE3D22900A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti135 – 1417NPMPNFK → QPDAELQ in AAB71350. (PubMed:9294447)Curated
    Sequence conflicti157 – 1571P → A in AAB71350. (PubMed:9294447)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF011894 Genomic DNA. Translation: AAB71350.1.
    AL591688 Genomic DNA. Translation: CAC45498.1.
    RefSeqiNP_385032.1. NC_003047.1.

    Genome annotation databases

    EnsemblBacteriaiCAC45498; CAC45498; SMc00021.
    GeneIDi1232567.
    KEGGisme:SMc00021.
    PATRICi23631121. VBISinMel96828_2324.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF011894 Genomic DNA. Translation: AAB71350.1 .
    AL591688 Genomic DNA. Translation: CAC45498.1 .
    RefSeqi NP_385032.1. NC_003047.1.

    3D structure databases

    ProteinModelPortali O30569.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 266834.SMc00021.

    Protein family/group databases

    REBASEi 3264. M.SmeI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC45498 ; CAC45498 ; SMc00021 .
    GeneIDi 1232567.
    KEGGi sme:SMc00021.
    PATRICi 23631121. VBISinMel96828_2324.

    Phylogenomic databases

    eggNOGi COG0863.
    HOGENOMi HOG000125329.
    KOi K13581.
    OMAi FWHFEDG.
    OrthoDBi EOG6FV84T.

    Enzyme and pathway databases

    BioCyci SMEL266834:GJF6-946-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01555. N6_N4_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00508. S21N4MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The CcrM DNA methyltransferase is widespread in the alpha subdivision of proteobacteria, and its essential functions are conserved in Rhizobium meliloti and Caulobacter crescentus."
      Wright R., Stephens C., Shapiro L.
      J. Bacteriol. 179:5869-5877(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 1021.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.

    Entry informationi

    Entry nameiMTS1_RHIME
    AccessioniPrimary (citable) accession number: O30569
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3