Reviewed, UniProtKB/Swiss-Prot O30569 (MTS1_RHIME)

Names and origin

Protein names

Recommended name:
    Modification methylase SmeI
      Short name=M.SmeI
    EC=2.1.1.72
Alternative name(s):
    Modification methylase SmeIP
      Short name=M.SmeIP
    Adenine-specific methyltransferase SmeIP
    M.CcrMI ortholog

Gene names

Name:	smeIM
Synonyms:	ccrM
Ordered Locus Names:	R00926
ORF Names:	SMc00021

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Modification methylase SmeI		PRO_0000087988
Experimental info
	Sequence conflict	135 – 141	7	NPMPNFK → QPDAELQ in AAB71350. Ref.1
	Sequence conflict	157	1	P → A in AAB71350. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O30569-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 790DE7FE3D22900A	FASTA	376	41,442
	10 20 30 40 50 60 MSSVVSLAEI SRAARPLNWL DSIIKGDCVA ALNALPDHSV DVVFADPPYN LQLGGTLHRP 70 80 90 100 110 120 DQSLVDAVDD DWDQFASFEA YDAFTRAWLL ACRRVLKPTG TLWVIGSYHN IFRVGAILQD 130 140 150 160 170 180 LHFWVLNDII WRKTNPMPNF KGRRFQNAHE TLIWATPNAK AKGYTFNYEA MKAANDDVQM 190 200 210 220 230 240 RSDWLFPICS GSERLKGDDG KKVHPTQKPE ALLARILMAS TKPGDVVLDP FFGSGTTGAV 250 260 270 280 290 300 AKRLGRHFVG IEREQDYIDA AAERIAAVEP LGKATLSVMT GKKAEPRVAF NTLVESGLIK 310 320 330 340 350 360 PGTVLTDAKR RYSAIVRADG TLASGGEAGS IHRLGAKVQG LDACNGWTFW HFEEGSVLKP 370 IDELRSVIRN DLAKLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The CcrM DNA methyltransferase is widespread in the alpha subdivision of proteobacteria, and its essential functions are conserved in Rhizobium meliloti and Caulobacter crescentus." Wright R., Stephens C., Shapiro L. J. Bacteriol. 179:5869-5877(1997) [PubMed: 9294447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1021.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF011894 Genomic DNA. Translation: AAB71350.1. AL591688 Genomic DNA. Translation: CAC45498.1.
RefSeq	NP_385032.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3264. M.SmeI.
Genome annotation databases
GeneID	1232567.
GenomeReviews	Gene locus R00926 in contig AL591688_GR.
KEGG	sme:SMc00021.
NMPDR	fig|266834.1.peg.2220.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG501679.
OMA	WRKANPM.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC00021-MONOMER.
BRENDA	2.1.1.72. 142.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. IPR001091. MeTrfase_CN4. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTS1_RHIME

Accession

Primary (citable) accession number: O30569

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	January 19, 2010
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)