Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O30569

- MTS1_RHIME

UniProt

O30569 - MTS1_RHIME

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Modification methylase SmeI

Gene

smeIM

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-946-MONOMER.

Protein family/group databases

REBASEi3264. M.SmeI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase SmeI (EC:2.1.1.72)
Short name:
M.SmeI
Alternative name(s):
Adenine-specific methyltransferase SmeIP
M.CcrMI ortholog
Modification methylase SmeIP
Short name:
M.SmeIP
Gene namesi
Name:smeIM
Synonyms:ccrM
Ordered Locus Names:R00926
ORF Names:SMc00021
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001976: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Modification methylase SmeIPRO_0000087988Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi266834.SMc00021.

Structurei

3D structure databases

ProteinModelPortaliO30569.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0863.
HOGENOMiHOG000125329.
KOiK13581.
OMAiFWHFEDG.
OrthoDBiEOG6FV84T.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O30569-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSVVSLAEI SRAARPLNWL DSIIKGDCVA ALNALPDHSV DVVFADPPYN
60 70 80 90 100
LQLGGTLHRP DQSLVDAVDD DWDQFASFEA YDAFTRAWLL ACRRVLKPTG
110 120 130 140 150
TLWVIGSYHN IFRVGAILQD LHFWVLNDII WRKTNPMPNF KGRRFQNAHE
160 170 180 190 200
TLIWATPNAK AKGYTFNYEA MKAANDDVQM RSDWLFPICS GSERLKGDDG
210 220 230 240 250
KKVHPTQKPE ALLARILMAS TKPGDVVLDP FFGSGTTGAV AKRLGRHFVG
260 270 280 290 300
IEREQDYIDA AAERIAAVEP LGKATLSVMT GKKAEPRVAF NTLVESGLIK
310 320 330 340 350
PGTVLTDAKR RYSAIVRADG TLASGGEAGS IHRLGAKVQG LDACNGWTFW
360 370
HFEEGSVLKP IDELRSVIRN DLAKLN
Length:376
Mass (Da):41,442
Last modified:May 30, 2000 - v2
Checksum:i790DE7FE3D22900A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti135 – 1417NPMPNFK → QPDAELQ in AAB71350. (PubMed:9294447)Curated
Sequence conflicti157 – 1571P → A in AAB71350. (PubMed:9294447)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF011894 Genomic DNA. Translation: AAB71350.1.
AL591688 Genomic DNA. Translation: CAC45498.1.
RefSeqiNP_385032.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC45498; CAC45498; SMc00021.
GeneIDi1232567.
KEGGisme:SMc00021.
PATRICi23631121. VBISinMel96828_2324.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF011894 Genomic DNA. Translation: AAB71350.1 .
AL591688 Genomic DNA. Translation: CAC45498.1 .
RefSeqi NP_385032.1. NC_003047.1.

3D structure databases

ProteinModelPortali O30569.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 266834.SMc00021.

Protein family/group databases

REBASEi 3264. M.SmeI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC45498 ; CAC45498 ; SMc00021 .
GeneIDi 1232567.
KEGGi sme:SMc00021.
PATRICi 23631121. VBISinMel96828_2324.

Phylogenomic databases

eggNOGi COG0863.
HOGENOMi HOG000125329.
KOi K13581.
OMAi FWHFEDG.
OrthoDBi EOG6FV84T.

Enzyme and pathway databases

BioCyci SMEL266834:GJF6-946-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01555. N6_N4_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00508. S21N4MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The CcrM DNA methyltransferase is widespread in the alpha subdivision of proteobacteria, and its essential functions are conserved in Rhizobium meliloti and Caulobacter crescentus."
    Wright R., Stephens C., Shapiro L.
    J. Bacteriol. 179:5869-5877(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 1021.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.

Entry informationi

Entry nameiMTS1_RHIME
AccessioniPrimary (citable) accession number: O30569
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: October 1, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3