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Protein

Cyclomaltodextrin glucanotransferase

Gene

cgt

Organism
Brevibacillus brevis (Bacillus brevis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.

Cofactori

Ca2+By similarityNote: Binds 2 calcium ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43Calcium 1By similarity1
Metal bindingi45Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi48Calcium 1By similarity1
Metal bindingi49Calcium 1By similarity1
Metal bindingi67Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi69Calcium 1By similarity1
Metal bindingi152Calcium 2By similarity1
Binding sitei153SubstrateBy similarity1
Metal bindingi203Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi212Calcium 2By similarity1
Binding sitei239SubstrateBy similarity1
Active sitei241NucleophileBy similarity1
Metal bindingi245Calcium 2; via carbonyl oxygenBy similarity1
Active sitei269Proton donorBy similarity1
Binding sitei339SubstrateBy similarity1
Sitei340Transition state stabilizerBy similarity1
Binding sitei382SubstrateBy similarity1
Binding sitei386SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.19. 638.

Protein family/group databases

CAZyiCBM20. Carbohydrate-Binding Module Family 20.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclomaltodextrin glucanotransferase (EC:2.4.1.19)
Alternative name(s):
Cyclodextrin-glycosyltransferase
Short name:
CGTase
Gene namesi
Name:cgt
OrganismiBrevibacillus brevis (Bacillus brevis)
Taxonomic identifieri1393 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000000142921 – 692Cyclomaltodextrin glucanotransferaseAdd BLAST672

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi59 ↔ 66By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliO30565.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini509 – 587IPT/TIGAdd BLAST79
Domaini588 – 692CBM20PROSITE-ProRule annotationAdd BLAST105

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni113 – 114Substrate bindingBy similarity2
Regioni206 – 209Substrate bindingBy similarity4
Regioni244 – 245Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated
Contains 1 CBM20 (carbohydrate binding type-20) domain.PROSITE-ProRule annotation
Contains 1 IPT/TIG domain.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF00686. CBM_20. 1 hit.
PF01833. TIG. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
SM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O30565-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITPSFIISS FLSLPTVVEA SVTNKVNYSK DVIYQIVTDR FSDGNPANNP
60 70 80 90 100
SGAIFSQNCS DLHKYCGGDW QGIINKMNDG YLTDLGITAL WISQPVENVY
110 120 130 140 150
ALHPSGYTSY HGYWARDYKK TNPYFGNFSD FDRLVSTAHN KGIKIIMDFT
160 170 180 190 200
PNHSSLALET NPNYVENGAL YNNGALLGNY SNDRNKLFHH NGGTDFSSYE
210 220 230 240 250
DSIYRNLYDL ADYDLNNKVV DQYLKESIKL WLIKIDGIRV DAVKHMSEGW
260 270 280 290 300
QTSLMSDIYT YKPVFTFGEW FLGTGEVDPQ NHHFANESGM SLLDFQFGQT
310 320 330 340 350
IRSVLKDRTS NWYDFNEMIK STEKDYDEVI DQVTFIDNHD MSRFSMVVFN
360 370 380 390 400
FQTDIALAVL LTSRGVPTIY YGTEQYLTGG NDPDNRKPMK TFDRSTNSYK
410 420 430 440 450
ITSKLASLRQ RNSALGYGNT TERWINSDVY IYERKFGNSI VLTAVNSSNR
460 470 480 490 500
NQTISNLNTS LPQGNYTDEL QQLLDGNTIT VNANGSANSP QLQANSVAVW
510 520 530 540 550
QVTKESTSPL IGHVGPMIGK TGNTVTVSGE GFGDKKGSVL FGSTSAEIVS
560 570 580 590 600
WSNTEIQVKV PNVTAGHYNL SVVNATNTKS PAYEKFEVLS GNQVSVRFAV
610 620 630 640 650
NNATTNSGTN VYIVGNVSEL GNWDPNKAIG PMFNQVMYKY PTWYYDISVP
660 670 680 690
AGKNLEYKYI KKDHNGNVTW QSGNNRTYTS PATGTDTVIS NW
Length:692
Mass (Da):77,405
Last modified:January 1, 1998 - v1
Checksum:i8CF5AD8CB9EFF57E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011388 Genomic DNA. Translation: AAB65420.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011388 Genomic DNA. Translation: AAB65420.1.

3D structure databases

ProteinModelPortaliO30565.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM20. Carbohydrate-Binding Module Family 20.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.4.1.19. 638.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF00686. CBM_20. 1 hit.
PF01833. TIG. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
SM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDGT_BREBE
AccessioniPrimary (citable) accession number: O30565
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: January 1, 1998
Last modified: October 5, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.