Reviewed, UniProtKB/Swiss-Prot O30565 (CDGT_BREBE)

Names and origin

Protein names

Recommended name:
    Cyclomaltodextrin glucanotransferase
    EC=2.4.1.19
Alternative name(s):
    Cyclodextrin-glycosyltransferase
      Short name=CGTase

Gene names

Name:

cgt

Organism

Brevibacillus brevis (Bacillus brevis)

Taxonomic identifier

1393 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Paenibacillaceae › Brevibacillus

Protein attributes

Sequence length	692 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. Contains 1 CBM20 (carbohydrate binding type-20) domain. Contains 1 IPT/TIG domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate binding Inferred from electronic annotation. Source: InterPro cyclomaltodextrin glucanotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 692	672	Cyclomaltodextrin glucanotransferase		PRO_0000001429
Regions
	Domain	509 – 587	79	IPT/TIG
	Domain	588 – 692	105	CBM20
Sites
	Active site	241	1	Nucleophile By similarity
	Active site	269	1	Proton donor By similarity
	Active site	340	1	By similarity
	Metal binding	43	1	Calcium 2 By similarity
	Metal binding	45	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	48	1	Calcium 2 By similarity
	Metal binding	49	1	Calcium 2 By similarity
	Metal binding	67	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	69	1	Calcium 2 By similarity
	Metal binding	152	1	Calcium 1 By similarity
	Metal binding	203	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	212	1	Calcium 1 By similarity
	Metal binding	245	1	Calcium 1; via carbonyl oxygen By similarity
Amino acid modifications
	Disulfide bond	59 ↔ 66		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O30565-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 8CF5AD8CB9EFF57E	FASTA	692	77,405
	10 20 30 40 50 60 MITPSFIISS FLSLPTVVEA SVTNKVNYSK DVIYQIVTDR FSDGNPANNP SGAIFSQNCS 70 80 90 100 110 120 DLHKYCGGDW QGIINKMNDG YLTDLGITAL WISQPVENVY ALHPSGYTSY HGYWARDYKK 130 140 150 160 170 180 TNPYFGNFSD FDRLVSTAHN KGIKIIMDFT PNHSSLALET NPNYVENGAL YNNGALLGNY 190 200 210 220 230 240 SNDRNKLFHH NGGTDFSSYE DSIYRNLYDL ADYDLNNKVV DQYLKESIKL WLIKIDGIRV 250 260 270 280 290 300 DAVKHMSEGW QTSLMSDIYT YKPVFTFGEW FLGTGEVDPQ NHHFANESGM SLLDFQFGQT 310 320 330 340 350 360 IRSVLKDRTS NWYDFNEMIK STEKDYDEVI DQVTFIDNHD MSRFSMVVFN FQTDIALAVL 370 380 390 400 410 420 LTSRGVPTIY YGTEQYLTGG NDPDNRKPMK TFDRSTNSYK ITSKLASLRQ RNSALGYGNT 430 440 450 460 470 480 TERWINSDVY IYERKFGNSI VLTAVNSSNR NQTISNLNTS LPQGNYTDEL QQLLDGNTIT 490 500 510 520 530 540 VNANGSANSP QLQANSVAVW QVTKESTSPL IGHVGPMIGK TGNTVTVSGE GFGDKKGSVL 550 560 570 580 590 600 FGSTSAEIVS WSNTEIQVKV PNVTAGHYNL SVVNATNTKS PAYEKFEVLS GNQVSVRFAV 610 620 630 640 650 660 NNATTNSGTN VYIVGNVSEL GNWDPNKAIG PMFNQVMYKY PTWYYDISVP AGKNLEYKYI 670 680 690 KKDHNGNVTW QSGNNRTYTS PATGTDTVIS NW

References

[1]	"Cloning and sequencing of a cyclodextrin glycosyltransferase gene from Brevibacillus brevis CD162 and its expression in Escherichia coli." Kim M.H., Sohn C.B., Oh T.K. FEMS Microbiol. Lett. 164:411-418(1998) [PubMed: 9682490] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CD162.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF011388 Genomic DNA. Translation: AAB65420.1.
3D structure databases
HSSP	HSSP built from PDB template 1CYG based on UniProtKB P31797.
ModBase	Search...
Protein family/group databases
CAZy	CBM20. Carbohydrate-Binding Module Family 20. GH13. Glycoside Hydrolase Family 13.
Enzyme and pathway databases
BRENDA	2.4.1.19. 289125.
Family and domain databases
InterPro	IPR006048. A-amylase_b_C. IPR006046. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR006589. Glyco_hydro_13_sub_cat. IPR002044. Glyco_hydro_carb-bd. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR002909. IPT_TIG_rcpt. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF00686. CBM_20. 1 hit. PF01833. TIG. 1 hit. [Graphical view]
PRINTS	PR00110. ALPHAAMYLASE.
ProDom	PD001568. Glyco_hydro_CBD. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00642. Aamy. 1 hit. SM00632. Aamy_C. 1 hit. [Graphical view]
PROSITE	PS51166. CBM20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDGT_BREBE

Accession

Primary (citable) accession number: O30565

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)