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Protein

Cyclomaltodextrin glucanotransferase

Gene

cgt

Organism
Brevibacillus brevis (Bacillus brevis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.

Cofactori

Ca2+By similarityNote: Binds 2 calcium ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431Calcium 1By similarity
Metal bindingi45 – 451Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi48 – 481Calcium 1By similarity
Metal bindingi49 – 491Calcium 1By similarity
Metal bindingi67 – 671Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi69 – 691Calcium 1By similarity
Metal bindingi152 – 1521Calcium 2By similarity
Binding sitei153 – 1531SubstrateBy similarity
Metal bindingi203 – 2031Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi212 – 2121Calcium 2By similarity
Binding sitei239 – 2391SubstrateBy similarity
Active sitei241 – 2411NucleophileBy similarity
Metal bindingi245 – 2451Calcium 2; via carbonyl oxygenBy similarity
Active sitei269 – 2691Proton donorBy similarity
Binding sitei339 – 3391SubstrateBy similarity
Sitei340 – 3401Transition state stabilizerBy similarity
Binding sitei382 – 3821SubstrateBy similarity
Binding sitei386 – 3861SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.19. 638.

Protein family/group databases

CAZyiCBM20. Carbohydrate-Binding Module Family 20.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclomaltodextrin glucanotransferase (EC:2.4.1.19)
Alternative name(s):
Cyclodextrin-glycosyltransferase
Short name:
CGTase
Gene namesi
Name:cgt
OrganismiBrevibacillus brevis (Bacillus brevis)
Taxonomic identifieri1393 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 692672Cyclomaltodextrin glucanotransferasePRO_0000001429Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi59 ↔ 66By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliO30565.
SMRiO30565. Positions 24-692.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini509 – 58779IPT/TIGAdd
BLAST
Domaini588 – 692105CBM20PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni113 – 1142Substrate bindingBy similarity
Regioni206 – 2094Substrate bindingBy similarity
Regioni244 – 2452Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated
Contains 1 CBM20 (carbohydrate binding type-20) domain.PROSITE-ProRule annotation
Contains 1 IPT/TIG domain.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF00686. CBM_20. 1 hit.
PF01833. TIG. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
SM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O30565-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITPSFIISS FLSLPTVVEA SVTNKVNYSK DVIYQIVTDR FSDGNPANNP
60 70 80 90 100
SGAIFSQNCS DLHKYCGGDW QGIINKMNDG YLTDLGITAL WISQPVENVY
110 120 130 140 150
ALHPSGYTSY HGYWARDYKK TNPYFGNFSD FDRLVSTAHN KGIKIIMDFT
160 170 180 190 200
PNHSSLALET NPNYVENGAL YNNGALLGNY SNDRNKLFHH NGGTDFSSYE
210 220 230 240 250
DSIYRNLYDL ADYDLNNKVV DQYLKESIKL WLIKIDGIRV DAVKHMSEGW
260 270 280 290 300
QTSLMSDIYT YKPVFTFGEW FLGTGEVDPQ NHHFANESGM SLLDFQFGQT
310 320 330 340 350
IRSVLKDRTS NWYDFNEMIK STEKDYDEVI DQVTFIDNHD MSRFSMVVFN
360 370 380 390 400
FQTDIALAVL LTSRGVPTIY YGTEQYLTGG NDPDNRKPMK TFDRSTNSYK
410 420 430 440 450
ITSKLASLRQ RNSALGYGNT TERWINSDVY IYERKFGNSI VLTAVNSSNR
460 470 480 490 500
NQTISNLNTS LPQGNYTDEL QQLLDGNTIT VNANGSANSP QLQANSVAVW
510 520 530 540 550
QVTKESTSPL IGHVGPMIGK TGNTVTVSGE GFGDKKGSVL FGSTSAEIVS
560 570 580 590 600
WSNTEIQVKV PNVTAGHYNL SVVNATNTKS PAYEKFEVLS GNQVSVRFAV
610 620 630 640 650
NNATTNSGTN VYIVGNVSEL GNWDPNKAIG PMFNQVMYKY PTWYYDISVP
660 670 680 690
AGKNLEYKYI KKDHNGNVTW QSGNNRTYTS PATGTDTVIS NW
Length:692
Mass (Da):77,405
Last modified:January 1, 1998 - v1
Checksum:i8CF5AD8CB9EFF57E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011388 Genomic DNA. Translation: AAB65420.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011388 Genomic DNA. Translation: AAB65420.1.

3D structure databases

ProteinModelPortaliO30565.
SMRiO30565. Positions 24-692.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM20. Carbohydrate-Binding Module Family 20.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.4.1.19. 638.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF00686. CBM_20. 1 hit.
PF01833. TIG. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
SM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDGT_BREBE
AccessioniPrimary (citable) accession number: O30565
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: January 1, 1998
Last modified: May 11, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.