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Protein

Cyclomaltodextrin glucanotransferase

Gene

cgt

Organism
Brevibacillus brevis (Bacillus brevis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.

Cofactori

Ca2+By similarityNote: Binds 2 calcium ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43Calcium 1By similarity1
Metal bindingi45Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi48Calcium 1By similarity1
Metal bindingi49Calcium 1By similarity1
Metal bindingi67Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi69Calcium 1By similarity1
Metal bindingi152Calcium 2By similarity1
Binding sitei153SubstrateBy similarity1
Metal bindingi203Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi212Calcium 2By similarity1
Binding sitei239SubstrateBy similarity1
Active sitei241NucleophileBy similarity1
Metal bindingi245Calcium 2; via carbonyl oxygenBy similarity1
Active sitei269Proton donorBy similarity1
Binding sitei339SubstrateBy similarity1
Sitei340Transition state stabilizerBy similarity1
Binding sitei382SubstrateBy similarity1
Binding sitei386SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.19 638

Protein family/group databases

CAZyiCBM20 Carbohydrate-Binding Module Family 20
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclomaltodextrin glucanotransferase (EC:2.4.1.19)
Alternative name(s):
Cyclodextrin-glycosyltransferase
Short name:
CGTase
Gene namesi
Name:cgt
OrganismiBrevibacillus brevis (Bacillus brevis)
Taxonomic identifieri1393 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000000142921 – 692Cyclomaltodextrin glucanotransferaseAdd BLAST672

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi59 ↔ 66By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiO30565

Structurei

3D structure databases

ProteinModelPortaliO30565
SMRiO30565
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini509 – 587IPT/TIGAdd BLAST79
Domaini588 – 692CBM20PROSITE-ProRule annotationAdd BLAST105

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni113 – 114Substrate bindingBy similarity2
Regioni206 – 209Substrate bindingBy similarity4
Regioni244 – 245Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.10, 2 hits
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR013784 Carb-bd-like_fold
IPR002044 CBM_fam20
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF00686 CBM_20, 1 hit
PF01833 TIG, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SM01065 CBM_2, 1 hit
SUPFAMiSSF49452 SSF49452, 1 hit
SSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS51166 CBM20, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O30565-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITPSFIISS FLSLPTVVEA SVTNKVNYSK DVIYQIVTDR FSDGNPANNP
60 70 80 90 100
SGAIFSQNCS DLHKYCGGDW QGIINKMNDG YLTDLGITAL WISQPVENVY
110 120 130 140 150
ALHPSGYTSY HGYWARDYKK TNPYFGNFSD FDRLVSTAHN KGIKIIMDFT
160 170 180 190 200
PNHSSLALET NPNYVENGAL YNNGALLGNY SNDRNKLFHH NGGTDFSSYE
210 220 230 240 250
DSIYRNLYDL ADYDLNNKVV DQYLKESIKL WLIKIDGIRV DAVKHMSEGW
260 270 280 290 300
QTSLMSDIYT YKPVFTFGEW FLGTGEVDPQ NHHFANESGM SLLDFQFGQT
310 320 330 340 350
IRSVLKDRTS NWYDFNEMIK STEKDYDEVI DQVTFIDNHD MSRFSMVVFN
360 370 380 390 400
FQTDIALAVL LTSRGVPTIY YGTEQYLTGG NDPDNRKPMK TFDRSTNSYK
410 420 430 440 450
ITSKLASLRQ RNSALGYGNT TERWINSDVY IYERKFGNSI VLTAVNSSNR
460 470 480 490 500
NQTISNLNTS LPQGNYTDEL QQLLDGNTIT VNANGSANSP QLQANSVAVW
510 520 530 540 550
QVTKESTSPL IGHVGPMIGK TGNTVTVSGE GFGDKKGSVL FGSTSAEIVS
560 570 580 590 600
WSNTEIQVKV PNVTAGHYNL SVVNATNTKS PAYEKFEVLS GNQVSVRFAV
610 620 630 640 650
NNATTNSGTN VYIVGNVSEL GNWDPNKAIG PMFNQVMYKY PTWYYDISVP
660 670 680 690
AGKNLEYKYI KKDHNGNVTW QSGNNRTYTS PATGTDTVIS NW
Length:692
Mass (Da):77,405
Last modified:January 1, 1998 - v1
Checksum:i8CF5AD8CB9EFF57E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011388 Genomic DNA Translation: AAB65420.1

Similar proteinsi

Entry informationi

Entry nameiCDGT_BREBE
AccessioniPrimary (citable) accession number: O30565
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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