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O30477

- DNAB_RHOMR

UniProt

O30477 - DNAB_RHOMR

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Protein
Replicative DNA helicase
Gene
dnaB
Organism
Rhodothermus marinus (Rhodothermus obamensis)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity By similarity.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi245 – 2528ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA helicase activity Source: InterPro
  4. endonuclease activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
  2. intein-mediated protein splicing Source: InterPro
  3. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

DNA replication, Intron homing

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Protein family/group databases

MEROPSiN10.002.
REBASEi4095. PI-Rma43812IP.

Names & Taxonomyi

Protein namesi
Recommended name:
Replicative DNA helicase (EC:3.6.4.12)
Cleaved into the following chain:
Alternative name(s):
Rma dnaB intein
Gene namesi
Name:dnaB
OrganismiRhodothermus marinus (Rhodothermus obamensis)
Taxonomic identifieri29549 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidetes Order II. Incertae sedisRhodothermaceaeRhodothermus

Subcellular locationi

GO - Cellular componenti

  1. primosome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421Replicative DNA helicase, 1st part Reviewed prediction
PRO_0000013285Add
BLAST
Chaini422 – 849428Endonuclease PI-Rma43812IP Reviewed prediction
PRO_0000013286Add
BLAST
Chaini850 – 94192Replicative DNA helicase, 2nd part Reviewed prediction
PRO_0000013287Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Reviewed prediction.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Structurei

3D structure databases

ProteinModelPortaliO30477.
SMRiO30477. Positions 790-851.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini214 – 484271SF4 helicase; first part
Add
BLAST
Domaini534 – 683150DOD-type homing endonuclease
Add
BLAST
Domaini646 – 915270SF4 helicase; second part
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.10.860.10. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF48024. SSF48024. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR00665. DnaB. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O30477-1 [UniParc]FASTAAdd to Basket

« Hide

MAEFEERPRL SIGEEEAPPY PLEKLTGGRR TRAQIHALHQ QAGRVPPQAV    50
ELEQAVLGAM LIEPEAIPRA LEILTPEAFY DGRHQRIFRA IVRLFEQNRG 100
VDLLTVTEEL RRTGELEQAG DTIYLSELTT RVASAANVEY HARIIAEKLL 150
RRMIEVMTLL VGRAYDPAAD AFELLDEVEA EIFRLSDVHL RKAARSMNEV 200
VKETLERLEA IHGRPGGITG VPSGFHQLDA LTGGWQRGDL IIIAARPSMG 250
KTAFALSCRN AALHPHYGTG VAIFSLEMGA EQLAQRLLTA EAASMPRRPA 300
PDGCATRTGV SWPARRPLSD APIFIDDTPS LGVLELRAKC RRLKAEHDIG 350
LVIVDYLQLM QASHMPRNAN REQEIAQISR SLKALAKELN VPVVALSQLS 400
RAVETRGGDK RPQLSDLRES GCLAGDTLIT LADGRRVPIR ELVSQQNFSV 450
WALNPQTYRL ERARVSRAFC TGIKPVYRLT TRLGRSIRAT ANHRFLTPQG 500
WKRVDELQPG DYLALPRRIP TASTPTLTEA ELALLGHLIG DGCTLPHHVI 550
QYTSRDADLA TLVAHLATKV FGSKVTPQIR KELRWYQVYL RAARPLAPGK 600
RNPISDWLRD LGIFGLRSYE KKVPALLFCQ TSEAIATFLR HLWATDGCIQ 650
MRRGKKPYPA VYYATSSYQL ARDVQSLLLR LGINARLKTV AQGEKGRVQY 700
HVKVSGREDL LRFVEKIGAV GARQRAALAS VYDYLSVRTG NPNRDIIPVA 750
LWYELVREAM YQRGISHRQL HANLGMAYGG MTLFRQNLSR ARALRLAEAA 800
ACPELRQLAQ SDVYWDPIVS IEPDGVEEVF DLTVPGPHNF VANDIIAHNS 850
IEQDADVVLF IYRPERYGIT VDENGNPTEG IAEIIIGKQR NGPTGTVRLA 900
FINQYARFEN LTMYQPEPGT PLPETPDETI LPSGPPDEAP F 941
Length:941
Mass (Da):104,883
Last modified:January 1, 1998 - v1
Checksum:i9145C41066152B29
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301R → RR in CAA74140. 1 Publication
Sequence conflicti148 – 1481K → KA in CAA74140. 1 Publication
Sequence conflicti258 – 2581C → CA in CAA74140. 1 Publication
Sequence conflicti293 – 31725ASMPR…PARRP → RVDAQAARTGRLRDEDWRKL ARAAGR in CAA74140. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF006675 Genomic DNA. Translation: AAB66912.1.
Y13813 Genomic DNA. Translation: CAA74140.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF006675 Genomic DNA. Translation: AAB66912.1 .
Y13813 Genomic DNA. Translation: CAA74140.1 .

3D structure databases

ProteinModelPortali O30477.
SMRi O30477. Positions 790-851.
ModBasei Search...

Protein family/group databases

MEROPSi N10.002.
REBASEi 4095. PI-Rma43812IP.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.860.10. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF48024. SSF48024. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR00665. DnaB. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A DnaB intein in Rhodothermus marinus: indication of recent intein homing across remotely related organisms."
    Liu X.Q., Hu Z.
    Proc. Natl. Acad. Sci. U.S.A. 94:7851-7856(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning of a dnaB homolog gene from Rhodothermus marinus with high similarity to the E.coli helicase but not able to complement a dnaBts mutation."
    Politz O.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiDNAB_RHOMR
AccessioniPrimary (citable) accession number: O30477
Secondary accession number(s): O50478
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi