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Protein

Replicative DNA helicase

Gene

dnaB

Organism
Rhodothermus marinus (Rhodothermus obamensis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi245 – 252ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Endonuclease, Helicase, Hydrolase, Nuclease
Biological processDNA replication, Intron homing
LigandATP-binding, Nucleotide-binding

Protein family/group databases

MEROPSiN10.002
REBASEi4095 PI-Rma43812IP

Names & Taxonomyi

Protein namesi
Recommended name:
Replicative DNA helicase (EC:3.6.4.12)
Cleaved into the following chain:
Alternative name(s):
Rma dnaB intein
Gene namesi
Name:dnaB
OrganismiRhodothermus marinus (Rhodothermus obamensis)
Taxonomic identifieri29549 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidetes Order II. Incertae sedisRhodothermaceaeRhodothermus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000132851 – 421Replicative DNA helicase, 1st partSequence analysisAdd BLAST421
ChainiPRO_0000013286422 – 849Endonuclease PI-Rma43812IPSequence analysisAdd BLAST428
ChainiPRO_0000013287850 – 941Replicative DNA helicase, 2nd partSequence analysisAdd BLAST92

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRIDEiO30477

Structurei

3D structure databases

ProteinModelPortaliO30477
SMRiO30477
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini214 – 484SF4 helicase; first partPROSITE-ProRule annotationAdd BLAST271
Domaini534 – 683DOD-type homing endonucleasePROSITE-ProRule annotationAdd BLAST150
Domaini646 – 915SF4 helicase; second partPROSITE-ProRule annotationAdd BLAST270

Sequence similaritiesi

Belongs to the helicase family. DnaB subfamily.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105CDU Bacteria
COG0305 LUCA
COG1372 LUCA

Family and domain databases

CDDicd00984 DnaB_C, 1 hit
Gene3Di1.10.860.10, 1 hit
3.10.28.10, 1 hit
InterProiView protein in InterPro
IPR036185 DNA_heli_DnaB-like_N_sf
IPR007692 DNA_helicase_DnaB
IPR007694 DNA_helicase_DnaB-like_C
IPR007693 DNA_helicase_DnaB-like_N
IPR016136 DNA_helicase_N/primase_C
IPR003586 Hint_dom_C
IPR003587 Hint_dom_N
IPR036844 Hint_dom_sf
IPR027434 Homing_endonucl
IPR006142 INTEIN
IPR030934 Intein_C
IPR004042 Intein_endonuc
IPR006141 Intein_N
IPR004860 LAGLIDADG_2
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00772 DnaB, 1 hit
PF03796 DnaB_C, 1 hit
PF14528 LAGLIDADG_3, 1 hit
PRINTSiPR00379 INTEIN
SMARTiView protein in SMART
SM00305 HintC, 1 hit
SM00306 HintN, 1 hit
SUPFAMiSSF48024 SSF48024, 1 hit
SSF51294 SSF51294, 2 hits
SSF52540 SSF52540, 1 hit
SSF55608 SSF55608, 1 hit
TIGRFAMsiTIGR00665 DnaB, 1 hit
TIGR01443 intein_Cterm, 1 hit
TIGR01445 intein_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS50818 INTEIN_C_TER, 1 hit
PS50819 INTEIN_ENDONUCLEASE, 1 hit
PS50817 INTEIN_N_TER, 1 hit
PS51199 SF4_HELICASE, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O30477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEFEERPRL SIGEEEAPPY PLEKLTGGRR TRAQIHALHQ QAGRVPPQAV
60 70 80 90 100
ELEQAVLGAM LIEPEAIPRA LEILTPEAFY DGRHQRIFRA IVRLFEQNRG
110 120 130 140 150
VDLLTVTEEL RRTGELEQAG DTIYLSELTT RVASAANVEY HARIIAEKLL
160 170 180 190 200
RRMIEVMTLL VGRAYDPAAD AFELLDEVEA EIFRLSDVHL RKAARSMNEV
210 220 230 240 250
VKETLERLEA IHGRPGGITG VPSGFHQLDA LTGGWQRGDL IIIAARPSMG
260 270 280 290 300
KTAFALSCRN AALHPHYGTG VAIFSLEMGA EQLAQRLLTA EAASMPRRPA
310 320 330 340 350
PDGCATRTGV SWPARRPLSD APIFIDDTPS LGVLELRAKC RRLKAEHDIG
360 370 380 390 400
LVIVDYLQLM QASHMPRNAN REQEIAQISR SLKALAKELN VPVVALSQLS
410 420 430 440 450
RAVETRGGDK RPQLSDLRES GCLAGDTLIT LADGRRVPIR ELVSQQNFSV
460 470 480 490 500
WALNPQTYRL ERARVSRAFC TGIKPVYRLT TRLGRSIRAT ANHRFLTPQG
510 520 530 540 550
WKRVDELQPG DYLALPRRIP TASTPTLTEA ELALLGHLIG DGCTLPHHVI
560 570 580 590 600
QYTSRDADLA TLVAHLATKV FGSKVTPQIR KELRWYQVYL RAARPLAPGK
610 620 630 640 650
RNPISDWLRD LGIFGLRSYE KKVPALLFCQ TSEAIATFLR HLWATDGCIQ
660 670 680 690 700
MRRGKKPYPA VYYATSSYQL ARDVQSLLLR LGINARLKTV AQGEKGRVQY
710 720 730 740 750
HVKVSGREDL LRFVEKIGAV GARQRAALAS VYDYLSVRTG NPNRDIIPVA
760 770 780 790 800
LWYELVREAM YQRGISHRQL HANLGMAYGG MTLFRQNLSR ARALRLAEAA
810 820 830 840 850
ACPELRQLAQ SDVYWDPIVS IEPDGVEEVF DLTVPGPHNF VANDIIAHNS
860 870 880 890 900
IEQDADVVLF IYRPERYGIT VDENGNPTEG IAEIIIGKQR NGPTGTVRLA
910 920 930 940
FINQYARFEN LTMYQPEPGT PLPETPDETI LPSGPPDEAP F
Length:941
Mass (Da):104,883
Last modified:January 1, 1998 - v1
Checksum:i9145C41066152B29
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30R → RR in CAA74140 (Ref. 2) Curated1
Sequence conflicti148K → KA in CAA74140 (Ref. 2) Curated1
Sequence conflicti258C → CA in CAA74140 (Ref. 2) Curated1
Sequence conflicti293 – 317ASMPR…PARRP → RVDAQAARTGRLRDEDWRKL ARAAGR in CAA74140 (Ref. 2) CuratedAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006675 Genomic DNA Translation: AAB66912.1
Y13813 Genomic DNA Translation: CAA74140.1

Similar proteinsi

Entry informationi

Entry nameiDNAB_RHOMR
AccessioniPrimary (citable) accession number: O30477
Secondary accession number(s): O50478
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

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