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O30477

- DNAB_RHOMR

UniProt

O30477 - DNAB_RHOMR

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Protein

Replicative DNA helicase

Gene

dnaB

Organism
Rhodothermus marinus (Rhodothermus obamensis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi245 – 2528ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA helicase activity Source: InterPro
  4. endonuclease activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
  2. intein-mediated protein splicing Source: InterPro
  3. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

DNA replication, Intron homing

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Protein family/group databases

REBASEi4095. PI-Rma43812IP.

Names & Taxonomyi

Protein namesi
Recommended name:
Replicative DNA helicase (EC:3.6.4.12)
Cleaved into the following chain:
Alternative name(s):
Rma dnaB intein
Gene namesi
Name:dnaB
OrganismiRhodothermus marinus (Rhodothermus obamensis)
Taxonomic identifieri29549 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidetes Order II. Incertae sedisRhodothermaceaeRhodothermus

Subcellular locationi

GO - Cellular componenti

  1. primosome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421Replicative DNA helicase, 1st partSequence AnalysisPRO_0000013285Add
BLAST
Chaini422 – 849428Endonuclease PI-Rma43812IPSequence AnalysisPRO_0000013286Add
BLAST
Chaini850 – 94192Replicative DNA helicase, 2nd partSequence AnalysisPRO_0000013287Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Structurei

3D structure databases

ProteinModelPortaliO30477.
SMRiO30477. Positions 790-851.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini214 – 484271SF4 helicase; first partPROSITE-ProRule annotationAdd
BLAST
Domaini534 – 683150DOD-type homing endonucleasePROSITE-ProRule annotationAdd
BLAST
Domaini646 – 915270SF4 helicase; second partPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. DnaB subfamily.Curated
Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation
Contains 1 SF4 helicase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.10.860.10. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF48024. SSF48024. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR00665. DnaB. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O30477-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEFEERPRL SIGEEEAPPY PLEKLTGGRR TRAQIHALHQ QAGRVPPQAV
60 70 80 90 100
ELEQAVLGAM LIEPEAIPRA LEILTPEAFY DGRHQRIFRA IVRLFEQNRG
110 120 130 140 150
VDLLTVTEEL RRTGELEQAG DTIYLSELTT RVASAANVEY HARIIAEKLL
160 170 180 190 200
RRMIEVMTLL VGRAYDPAAD AFELLDEVEA EIFRLSDVHL RKAARSMNEV
210 220 230 240 250
VKETLERLEA IHGRPGGITG VPSGFHQLDA LTGGWQRGDL IIIAARPSMG
260 270 280 290 300
KTAFALSCRN AALHPHYGTG VAIFSLEMGA EQLAQRLLTA EAASMPRRPA
310 320 330 340 350
PDGCATRTGV SWPARRPLSD APIFIDDTPS LGVLELRAKC RRLKAEHDIG
360 370 380 390 400
LVIVDYLQLM QASHMPRNAN REQEIAQISR SLKALAKELN VPVVALSQLS
410 420 430 440 450
RAVETRGGDK RPQLSDLRES GCLAGDTLIT LADGRRVPIR ELVSQQNFSV
460 470 480 490 500
WALNPQTYRL ERARVSRAFC TGIKPVYRLT TRLGRSIRAT ANHRFLTPQG
510 520 530 540 550
WKRVDELQPG DYLALPRRIP TASTPTLTEA ELALLGHLIG DGCTLPHHVI
560 570 580 590 600
QYTSRDADLA TLVAHLATKV FGSKVTPQIR KELRWYQVYL RAARPLAPGK
610 620 630 640 650
RNPISDWLRD LGIFGLRSYE KKVPALLFCQ TSEAIATFLR HLWATDGCIQ
660 670 680 690 700
MRRGKKPYPA VYYATSSYQL ARDVQSLLLR LGINARLKTV AQGEKGRVQY
710 720 730 740 750
HVKVSGREDL LRFVEKIGAV GARQRAALAS VYDYLSVRTG NPNRDIIPVA
760 770 780 790 800
LWYELVREAM YQRGISHRQL HANLGMAYGG MTLFRQNLSR ARALRLAEAA
810 820 830 840 850
ACPELRQLAQ SDVYWDPIVS IEPDGVEEVF DLTVPGPHNF VANDIIAHNS
860 870 880 890 900
IEQDADVVLF IYRPERYGIT VDENGNPTEG IAEIIIGKQR NGPTGTVRLA
910 920 930 940
FINQYARFEN LTMYQPEPGT PLPETPDETI LPSGPPDEAP F
Length:941
Mass (Da):104,883
Last modified:January 1, 1998 - v1
Checksum:i9145C41066152B29
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301R → RR in CAA74140. 1 PublicationCurated
Sequence conflicti148 – 1481K → KA in CAA74140. 1 PublicationCurated
Sequence conflicti258 – 2581C → CA in CAA74140. 1 PublicationCurated
Sequence conflicti293 – 31725ASMPR…PARRP → RVDAQAARTGRLRDEDWRKL ARAAGR in CAA74140. 1 PublicationCuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF006675 Genomic DNA. Translation: AAB66912.1.
Y13813 Genomic DNA. Translation: CAA74140.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF006675 Genomic DNA. Translation: AAB66912.1 .
Y13813 Genomic DNA. Translation: CAA74140.1 .

3D structure databases

ProteinModelPortali O30477.
SMRi O30477. Positions 790-851.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 4095. PI-Rma43812IP.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.860.10. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF48024. SSF48024. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR00665. DnaB. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A DnaB intein in Rhodothermus marinus: indication of recent intein homing across remotely related organisms."
    Liu X.Q., Hu Z.
    Proc. Natl. Acad. Sci. U.S.A. 94:7851-7856(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning of a dnaB homolog gene from Rhodothermus marinus with high similarity to the E.coli helicase but not able to complement a dnaBts mutation."
    Politz O.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiDNAB_RHOMR
AccessioniPrimary (citable) accession number: O30477
Secondary accession number(s): O50478
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3