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O30477

- DNAB_RHOMR

UniProt

O30477 - DNAB_RHOMR

Protein

Replicative DNA helicase

Gene

dnaB

Organism
Rhodothermus marinus (Rhodothermus obamensis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi245 – 2528ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. DNA helicase activity Source: InterPro
    4. endonuclease activity Source: UniProtKB-KW

    GO - Biological processi

    1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
    2. intein-mediated protein splicing Source: InterPro
    3. intron homing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Helicase, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA replication, Intron homing

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Protein family/group databases

    MEROPSiN10.002.
    REBASEi4095. PI-Rma43812IP.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicative DNA helicase (EC:3.6.4.12)
    Cleaved into the following chain:
    Alternative name(s):
    Rma dnaB intein
    Gene namesi
    Name:dnaB
    OrganismiRhodothermus marinus (Rhodothermus obamensis)
    Taxonomic identifieri29549 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidetes Order II. Incertae sedisRhodothermaceaeRhodothermus

    Subcellular locationi

    GO - Cellular componenti

    1. primosome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Primosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 421421Replicative DNA helicase, 1st partSequence AnalysisPRO_0000013285Add
    BLAST
    Chaini422 – 849428Endonuclease PI-Rma43812IPSequence AnalysisPRO_0000013286Add
    BLAST
    Chaini850 – 94192Replicative DNA helicase, 2nd partSequence AnalysisPRO_0000013287Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Structurei

    3D structure databases

    ProteinModelPortaliO30477.
    SMRiO30477. Positions 790-851.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini214 – 484271SF4 helicase; first partPROSITE-ProRule annotationAdd
    BLAST
    Domaini534 – 683150DOD-type homing endonucleasePROSITE-ProRule annotationAdd
    BLAST
    Domaini646 – 915270SF4 helicase; second partPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the helicase family. DnaB subfamily.Curated
    Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation
    Contains 1 SF4 helicase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di1.10.860.10. 1 hit.
    2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR007692. DNA_helicase_DnaB.
    IPR007694. DNA_helicase_DnaB-like_C.
    IPR007693. DNA_helicase_DnaB-like_N.
    IPR016136. DNA_helicase_N/primase_C.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00772. DnaB. 1 hit.
    PF03796. DnaB_C. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view]
    SUPFAMiSSF48024. SSF48024. 1 hit.
    SSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsiTIGR00665. DnaB. 1 hit.
    TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    PROSITEiPS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS51199. SF4_HELICASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O30477-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEFEERPRL SIGEEEAPPY PLEKLTGGRR TRAQIHALHQ QAGRVPPQAV    50
    ELEQAVLGAM LIEPEAIPRA LEILTPEAFY DGRHQRIFRA IVRLFEQNRG 100
    VDLLTVTEEL RRTGELEQAG DTIYLSELTT RVASAANVEY HARIIAEKLL 150
    RRMIEVMTLL VGRAYDPAAD AFELLDEVEA EIFRLSDVHL RKAARSMNEV 200
    VKETLERLEA IHGRPGGITG VPSGFHQLDA LTGGWQRGDL IIIAARPSMG 250
    KTAFALSCRN AALHPHYGTG VAIFSLEMGA EQLAQRLLTA EAASMPRRPA 300
    PDGCATRTGV SWPARRPLSD APIFIDDTPS LGVLELRAKC RRLKAEHDIG 350
    LVIVDYLQLM QASHMPRNAN REQEIAQISR SLKALAKELN VPVVALSQLS 400
    RAVETRGGDK RPQLSDLRES GCLAGDTLIT LADGRRVPIR ELVSQQNFSV 450
    WALNPQTYRL ERARVSRAFC TGIKPVYRLT TRLGRSIRAT ANHRFLTPQG 500
    WKRVDELQPG DYLALPRRIP TASTPTLTEA ELALLGHLIG DGCTLPHHVI 550
    QYTSRDADLA TLVAHLATKV FGSKVTPQIR KELRWYQVYL RAARPLAPGK 600
    RNPISDWLRD LGIFGLRSYE KKVPALLFCQ TSEAIATFLR HLWATDGCIQ 650
    MRRGKKPYPA VYYATSSYQL ARDVQSLLLR LGINARLKTV AQGEKGRVQY 700
    HVKVSGREDL LRFVEKIGAV GARQRAALAS VYDYLSVRTG NPNRDIIPVA 750
    LWYELVREAM YQRGISHRQL HANLGMAYGG MTLFRQNLSR ARALRLAEAA 800
    ACPELRQLAQ SDVYWDPIVS IEPDGVEEVF DLTVPGPHNF VANDIIAHNS 850
    IEQDADVVLF IYRPERYGIT VDENGNPTEG IAEIIIGKQR NGPTGTVRLA 900
    FINQYARFEN LTMYQPEPGT PLPETPDETI LPSGPPDEAP F 941
    Length:941
    Mass (Da):104,883
    Last modified:January 1, 1998 - v1
    Checksum:i9145C41066152B29
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti30 – 301R → RR in CAA74140. 1 PublicationCurated
    Sequence conflicti148 – 1481K → KA in CAA74140. 1 PublicationCurated
    Sequence conflicti258 – 2581C → CA in CAA74140. 1 PublicationCurated
    Sequence conflicti293 – 31725ASMPR…PARRP → RVDAQAARTGRLRDEDWRKL ARAAGR in CAA74140. 1 PublicationCuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF006675 Genomic DNA. Translation: AAB66912.1.
    Y13813 Genomic DNA. Translation: CAA74140.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF006675 Genomic DNA. Translation: AAB66912.1 .
    Y13813 Genomic DNA. Translation: CAA74140.1 .

    3D structure databases

    ProteinModelPortali O30477.
    SMRi O30477. Positions 790-851.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi N10.002.
    REBASEi 4095. PI-Rma43812IP.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.860.10. 1 hit.
    2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR007692. DNA_helicase_DnaB.
    IPR007694. DNA_helicase_DnaB-like_C.
    IPR007693. DNA_helicase_DnaB-like_N.
    IPR016136. DNA_helicase_N/primase_C.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00772. DnaB. 1 hit.
    PF03796. DnaB_C. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48024. SSF48024. 1 hit.
    SSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsi TIGR00665. DnaB. 1 hit.
    TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    PROSITEi PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS51199. SF4_HELICASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A DnaB intein in Rhodothermus marinus: indication of recent intein homing across remotely related organisms."
      Liu X.Q., Hu Z.
      Proc. Natl. Acad. Sci. U.S.A. 94:7851-7856(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Cloning of a dnaB homolog gene from Rhodothermus marinus with high similarity to the E.coli helicase but not able to complement a dnaBts mutation."
      Politz O.
      Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiDNAB_RHOMR
    AccessioniPrimary (citable) accession number: O30477
    Secondary accession number(s): O50478
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3