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O30477 (DNAB_RHOMR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Replicative DNA helicase

EC=3.6.4.12

Cleaved into the following chain:

  1. Endonuclease PI-Rma43812IP
    EC=3.1.-.-
    Alternative name(s):
    Rma dnaB intein
Gene names
Name:dnaB
OrganismRhodothermus marinus (Rhodothermus obamensis)
Taxonomic identifier29549 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidetes Order II. Incertae sedisRhodothermaceaeRhodothermus

Protein attributes

Sequence length941 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.

Sequence similarities

Belongs to the helicase family. DnaB subfamily.

Contains 1 DOD-type homing endonuclease domain.

Contains 1 SF4 helicase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 421421Replicative DNA helicase, 1st part Potential
PRO_0000013285
Chain422 – 849428Endonuclease PI-Rma43812IP Potential
PRO_0000013286
Chain850 – 94192Replicative DNA helicase, 2nd part Potential
PRO_0000013287

Regions

Domain214 – 484271SF4 helicase; first part
Domain534 – 683150DOD-type homing endonuclease
Domain646 – 915270SF4 helicase; second part
Nucleotide binding245 – 2528ATP Potential

Experimental info

Sequence conflict301R → RR in CAA74140. Ref.2
Sequence conflict1481K → KA in CAA74140. Ref.2
Sequence conflict2581C → CA in CAA74140. Ref.2
Sequence conflict293 – 31725ASMPR…PARRP → RVDAQAARTGRLRDEDWRKL ARAAGR in CAA74140. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O30477 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 9145C41066152B29

FASTA941104,883
        10         20         30         40         50         60 
MAEFEERPRL SIGEEEAPPY PLEKLTGGRR TRAQIHALHQ QAGRVPPQAV ELEQAVLGAM 

        70         80         90        100        110        120 
LIEPEAIPRA LEILTPEAFY DGRHQRIFRA IVRLFEQNRG VDLLTVTEEL RRTGELEQAG 

       130        140        150        160        170        180 
DTIYLSELTT RVASAANVEY HARIIAEKLL RRMIEVMTLL VGRAYDPAAD AFELLDEVEA 

       190        200        210        220        230        240 
EIFRLSDVHL RKAARSMNEV VKETLERLEA IHGRPGGITG VPSGFHQLDA LTGGWQRGDL 

       250        260        270        280        290        300 
IIIAARPSMG KTAFALSCRN AALHPHYGTG VAIFSLEMGA EQLAQRLLTA EAASMPRRPA 

       310        320        330        340        350        360 
PDGCATRTGV SWPARRPLSD APIFIDDTPS LGVLELRAKC RRLKAEHDIG LVIVDYLQLM 

       370        380        390        400        410        420 
QASHMPRNAN REQEIAQISR SLKALAKELN VPVVALSQLS RAVETRGGDK RPQLSDLRES 

       430        440        450        460        470        480 
GCLAGDTLIT LADGRRVPIR ELVSQQNFSV WALNPQTYRL ERARVSRAFC TGIKPVYRLT 

       490        500        510        520        530        540 
TRLGRSIRAT ANHRFLTPQG WKRVDELQPG DYLALPRRIP TASTPTLTEA ELALLGHLIG 

       550        560        570        580        590        600 
DGCTLPHHVI QYTSRDADLA TLVAHLATKV FGSKVTPQIR KELRWYQVYL RAARPLAPGK 

       610        620        630        640        650        660 
RNPISDWLRD LGIFGLRSYE KKVPALLFCQ TSEAIATFLR HLWATDGCIQ MRRGKKPYPA 

       670        680        690        700        710        720 
VYYATSSYQL ARDVQSLLLR LGINARLKTV AQGEKGRVQY HVKVSGREDL LRFVEKIGAV 

       730        740        750        760        770        780 
GARQRAALAS VYDYLSVRTG NPNRDIIPVA LWYELVREAM YQRGISHRQL HANLGMAYGG 

       790        800        810        820        830        840 
MTLFRQNLSR ARALRLAEAA ACPELRQLAQ SDVYWDPIVS IEPDGVEEVF DLTVPGPHNF 

       850        860        870        880        890        900 
VANDIIAHNS IEQDADVVLF IYRPERYGIT VDENGNPTEG IAEIIIGKQR NGPTGTVRLA 

       910        920        930        940 
FINQYARFEN LTMYQPEPGT PLPETPDETI LPSGPPDEAP F 

« Hide

References

[1]"A DnaB intein in Rhodothermus marinus: indication of recent intein homing across remotely related organisms."
Liu X.Q., Hu Z.
Proc. Natl. Acad. Sci. U.S.A. 94:7851-7856(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cloning of a dnaB homolog gene from Rhodothermus marinus with high similarity to the E.coli helicase but not able to complement a dnaBts mutation."
Politz O.
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF006675 Genomic DNA. Translation: AAB66912.1.
Y13813 Genomic DNA. Translation: CAA74140.1.

3D structure databases

ProteinModelPortalO30477.
SMRO30477. Positions 790-851.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSN10.002.
REBASE4095. PI-Rma43812IP.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.860.10. 1 hit.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF48024. SSF48024. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsTIGR00665. DnaB. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNAB_RHOMR
AccessionPrimary (citable) accession number: O30477
Secondary accession number(s): O50478
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries