O30477 (DNAB_RHOMR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Replicative DNA helicase
EC=3.6.4.12

Cleaved into the following chain:

1. Endonuclease PI-Rma43812IP
   EC=3.1.-.-
   Alternative name(s):
   Rma dnaB intein

Gene names

Name:

dnaB

Organism

Rhodothermus marinus (Rhodothermus obamensis)

Taxonomic identifier

29549 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Bacteroidetes Order II. Incertae sedis › Rhodothermaceae › Rhodothermus

Protein attributes

Sequence length	941 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the helicase family. DnaB subfamily. Contains 1 DOD-type homing endonuclease domain. Contains 1 SF4 helicase domain.

Ontologies

Keywords
Biological process	DNA replication Intron homing
Cellular component	Primosome
Domain	Repeat
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Endonuclease Helicase Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Gene Ontology (GO)
Biological_process	DNA duplex unwinding Inferred from electronic annotation. Source: GOC DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	replisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Replicative DNA helicase, 1st part Potential		PRO_0000013285
	Chain	422 – 849	428	Endonuclease PI-Rma43812IP Potential		PRO_0000013286
	Chain	850 – 941	92	Replicative DNA helicase, 2nd part Potential		PRO_0000013287
Regions
	Domain	214 – 484	271	SF4 helicase; first part
	Domain	534 – 683	150	DOD-type homing endonuclease
	Domain	646 – 915	270	SF4 helicase; second part
	Nucleotide binding	245 – 252	8	ATP Potential
Experimental info
	Sequence conflict	30	1	R → RR in CAA74140. Ref.2
	Sequence conflict	148	1	K → KA in CAA74140. Ref.2
	Sequence conflict	258	1	C → CA in CAA74140. Ref.2
	Sequence conflict	293 – 317	25	ASMPR…PARRP → RVDAQAARTGRLRDEDWRKL ARAAGR in CAA74140. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O30477 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 9145C41066152B29	FASTA	941	104,883
	10 20 30 40 50 60 MAEFEERPRL SIGEEEAPPY PLEKLTGGRR TRAQIHALHQ QAGRVPPQAV ELEQAVLGAM 70 80 90 100 110 120 LIEPEAIPRA LEILTPEAFY DGRHQRIFRA IVRLFEQNRG VDLLTVTEEL RRTGELEQAG 130 140 150 160 170 180 DTIYLSELTT RVASAANVEY HARIIAEKLL RRMIEVMTLL VGRAYDPAAD AFELLDEVEA 190 200 210 220 230 240 EIFRLSDVHL RKAARSMNEV VKETLERLEA IHGRPGGITG VPSGFHQLDA LTGGWQRGDL 250 260 270 280 290 300 IIIAARPSMG KTAFALSCRN AALHPHYGTG VAIFSLEMGA EQLAQRLLTA EAASMPRRPA 310 320 330 340 350 360 PDGCATRTGV SWPARRPLSD APIFIDDTPS LGVLELRAKC RRLKAEHDIG LVIVDYLQLM 370 380 390 400 410 420 QASHMPRNAN REQEIAQISR SLKALAKELN VPVVALSQLS RAVETRGGDK RPQLSDLRES 430 440 450 460 470 480 GCLAGDTLIT LADGRRVPIR ELVSQQNFSV WALNPQTYRL ERARVSRAFC TGIKPVYRLT 490 500 510 520 530 540 TRLGRSIRAT ANHRFLTPQG WKRVDELQPG DYLALPRRIP TASTPTLTEA ELALLGHLIG 550 560 570 580 590 600 DGCTLPHHVI QYTSRDADLA TLVAHLATKV FGSKVTPQIR KELRWYQVYL RAARPLAPGK 610 620 630 640 650 660 RNPISDWLRD LGIFGLRSYE KKVPALLFCQ TSEAIATFLR HLWATDGCIQ MRRGKKPYPA 670 680 690 700 710 720 VYYATSSYQL ARDVQSLLLR LGINARLKTV AQGEKGRVQY HVKVSGREDL LRFVEKIGAV 730 740 750 760 770 780 GARQRAALAS VYDYLSVRTG NPNRDIIPVA LWYELVREAM YQRGISHRQL HANLGMAYGG 790 800 810 820 830 840 MTLFRQNLSR ARALRLAEAA ACPELRQLAQ SDVYWDPIVS IEPDGVEEVF DLTVPGPHNF 850 860 870 880 890 900 VANDIIAHNS IEQDADVVLF IYRPERYGIT VDENGNPTEG IAEIIIGKQR NGPTGTVRLA 910 920 930 940 FINQYARFEN LTMYQPEPGT PLPETPDETI LPSGPPDEAP F

References

[1]	"A DnaB intein in Rhodothermus marinus: indication of recent intein homing across remotely related organisms." Liu X.Q., Hu Z. Proc. Natl. Acad. Sci. U.S.A. 94:7851-7856(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning of a dnaB homolog gene from Rhodothermus marinus with high similarity to the E.coli helicase but not able to complement a dnaBts mutation." Politz O. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF006675 Genomic DNA. Translation: AAB66912.1. Y13813 Genomic DNA. Translation: CAA74140.1.
3D structure databases
ProteinModelPortal	O30477.
SMR	O30477. Positions 790-851.
ModBase	Search...
Protein family/group databases
MEROPS	N10.002.
REBASE	4095. PI-Rma43812IP.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.860.10. 1 hit.
InterPro	IPR007692. DNA_helicase_DnaB. IPR007694. DNA_helicase_DnaB-like_C. IPR007693. DNA_helicase_DnaB-like_N. IPR016136. DNA_helicase_N/primase_C. IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. [Graphical view]
Pfam	PF00772. DnaB. 1 hit. PF03796. DnaB_C. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF48024. DNA_helicase_DnaB-like_N. 1 hit.
TIGRFAMs	TIGR00665. DnaB. 1 hit. TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS51199. SF4_HELICASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAB_RHOMR

Accession

Primary (citable) accession number: O30477
Secondary accession number(s): O50478

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program