Reviewed, UniProtKB/Swiss-Prot O30394 (TYSY_BACAT)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45
Alternative name(s):
    Thymidylate synthase A
      Short name=TS A
      Short name=TSase A

Gene names

Name:

thyA

Organism

Bacillus atrophaeus

Taxonomic identifier

1452 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	227 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 227	›227	Thymidylate synthase HAMAP MF_00008		PRO_0000140919
Sites
	Active site	109	1	By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	O30394-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 15B33930C329FAAB	FASTA	227	26,901
	10 20 30 40 50 60 SEVPILTTKK LAWKTAIKEL LWIWQLKSND VNDLNKMSVH IWDQWKQEDG TIGHAYGFQL 70 80 90 100 110 120 GKKNRSLNGE KVDQVDYLLH QLKNNPSSRR HITMLWNPDE LDAMALTPCV YETQWYVKHG 130 140 150 160 170 180 KLHLEVRARS NDMALGNPFN VFQYNVLQRM IAQVTGYELG EYIFNIGDCH VYTRHIDNLK 190 200 210 220 IQMEREQFEA PELWINPEVK DFYDFTIDDF KLINYKHGDK LLFEVRV

References

[1]	"Genes encoding thymidylate synthases A and B in the genus Bacillus are members of two distinct families." Tam N.H., Borriss R. Mol. Gen. Genet. 258:427-430(1998) [PubMed: 9648749] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S223 / B0099 / 1208.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF004101 Genomic DNA. Translation: AAC26323.1.
3D structure databases
HSSP	HSSP built from PDB template 1BKP based on UniProtKB P42326.
SMR	O30394. Positions 1-227.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.45. 306929.
Family and domain databases
HAMAP	MF_00008. [Tree]
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BACAT

Accession

Primary (citable) accession number: O30394

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)