O30394 (TYSY_BACAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Alternative name(s):
Thymidylate synthase A
Short name=TS A
Short name=TSase A

Gene names

Name:

thyA

Organism

Bacillus atrophaeus

Taxonomic identifier

1452 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group

Protein attributes

Sequence length	227 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 227	›227	Thymidylate synthase HAMAP-Rule MF_00008		PRO_0000140919
Sites
	Active site	109	1	By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	O30394 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 15B33930C329FAAB	FASTA	227	26,901
	10 20 30 40 50 60 SEVPILTTKK LAWKTAIKEL LWIWQLKSND VNDLNKMSVH IWDQWKQEDG TIGHAYGFQL 70 80 90 100 110 120 GKKNRSLNGE KVDQVDYLLH QLKNNPSSRR HITMLWNPDE LDAMALTPCV YETQWYVKHG 130 140 150 160 170 180 KLHLEVRARS NDMALGNPFN VFQYNVLQRM IAQVTGYELG EYIFNIGDCH VYTRHIDNLK 190 200 210 220 IQMEREQFEA PELWINPEVK DFYDFTIDDF KLINYKHGDK LLFEVRV

References

[1]	"Genes encoding thymidylate synthases A and B in the genus Bacillus are members of two distinct families." Tam N.H., Borriss R. Mol. Gen. Genet. 258:427-430(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S223 / B0099 / 1208.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF004101 Genomic DNA. Translation: AAC26323.1.
3D structure databases
ProteinModelPortal	O30394.
SMR	O30394. Positions 1-227.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 1 hit.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BACAT

Accession

Primary (citable) accession number: O30394

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program