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O30297 (NADK_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:AF_2373
OrganismArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) [Reference proteome] [HAMAP]
Taxonomic identifier224325 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus

Protein attributes

Sequence length249 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance between NAD(H) and NADP(H), and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP. Ref.2

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subunit structure

Homotetramer. Ref.2

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from direct assay Ref.2. Source: UniProtKB

NAD binding

Inferred from direct assay Ref.2. Source: UniProtKB

NAD+ kinase activity

Inferred from direct assay Ref.2. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 249249NAD kinase HAMAP-Rule MF_00361
PRO_0000120697

Regions

Nucleotide binding49 – 502NAD HAMAP-Rule MF_00361
Nucleotide binding115 – 1162NAD HAMAP-Rule MF_00361
Nucleotide binding156 – 1616NAD HAMAP-Rule MF_00361

Sites

Active site491Proton acceptor By similarity
Binding site541NAD
Binding site1261NAD
Binding site1431NAD
Binding site1451NAD Probable
Binding site1531NAD; via carbonyl oxygen
Binding site1801NAD; via carbonyl oxygen Probable
Binding site2111NAD

Secondary structure

............................................... 249
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O30297 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: E07E3163BD14FC7B

FASTA24927,868
        10         20         30         40         50         60 
MRAAVVYKTD GHVKRIEEAL KRLEVEVELF NQPSEELENF DFIVSVGGDG TILRILQKLK 

        70         80         90        100        110        120 
RCPPIFGINT GRVGLLTHAS PENFEVELKK AVEKFEVERF PRVSCSAMPD VLALNEIAVL 

       130        140        150        160        170        180 
SRKPAKMIDV ALRVDGVEVD RIRCDGFIVA TQIGSTGYAF SAGGPVVEPY LECFILIPIA 

       190        200        210        220        230        240 
PFRFGWKPYV VSMERKIEVI AEKAIVVADG QKSVDFDGEI TIEKSEFPAV FFKNEKRFRN 


LFGKVRSIG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000782 Genomic DNA. Translation: AAB91287.1.
PIRE69546.
RefSeqNP_071196.1. NC_000917.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1SUWX-ray2.45A/B/C/D1-249[»]
1Z0SX-ray1.70A/B/C/D1-249[»]
1Z0UX-ray2.00A/B1-249[»]
1Z0ZX-ray2.85A/B/C/D1-249[»]
ProteinModelPortalO30297.
SMRO30297. Positions 1-249.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224325.AF2373.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB91287; AAB91287; AF_2373.
GeneID1485603.
KEGGafu:AF2373.

Phylogenomic databases

eggNOGCOG0061.
KOK00858.
OMAWREWIAT.

Enzyme and pathway databases

BioCycAFUL224325:GJBC-2422-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceO30297.

Entry information

Entry nameNADK_ARCFU
AccessionPrimary (citable) accession number: O30297
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references