Reviewed, UniProtKB/Swiss-Prot O30297 (PPNK_ARCFU)

Names and origin

Protein names

Recommended name:
    Probable inorganic polyphosphate/ATP-NAD kinase
      Short name=Poly(P)/ATP NAD kinase
    EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	AF_2373

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity.
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP MF_00361
Cofactor	Divalent metal ions By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAD kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 249

249

Probable inorganic polyphosphate/ATP-NAD kinase HAMAP MF_00361

PRO_0000120697

Secondary structure

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249

Helix Strand Turn

Details...

Beta strand

2 – 10

9

Helix

13 – 21

9

Turn

22 – 24

3

Beta strand

26 – 32

7

Helix

35 – 39

5

Beta strand

40 – 47

8

Helix

49 – 56

8

Beta strand

65 – 69

5

Helix

81 – 83

3

Helix

84 – 94

11

Beta strand

97 – 100

4

Beta strand

103 – 108

6

Beta strand

113 – 123

11

Beta strand

128 – 134

7

Beta strand

137 – 151

15

Helix

154 – 156

3

Helix

158 – 161

4

Beta strand

174 – 180

7

Beta strand

189 – 191

3

Beta strand

197 – 208

12

Turn

209 – 211

3

Beta strand

212 – 224

13

Beta strand

229 – 232

4

Helix

237 – 245

9

Sequences

	Sequence		Length	Mass (Da)
	O30297-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: E07E3163BD14FC7B	FASTA	249	27,868
	10 20 30 40 50 60 MRAAVVYKTD GHVKRIEEAL KRLEVEVELF NQPSEELENF DFIVSVGGDG TILRILQKLK 70 80 90 100 110 120 RCPPIFGINT GRVGLLTHAS PENFEVELKK AVEKFEVERF PRVSCSAMPD VLALNEIAVL 130 140 150 160 170 180 SRKPAKMIDV ALRVDGVEVD RIRCDGFIVA TQIGSTGYAF SAGGPVVEPY LECFILIPIA 190 200 210 220 230 240 PFRFGWKPYV VSMERKIEVI AEKAIVVADG QKSVDFDGEI TIEKSEFPAV FFKNEKRFRN LFGKVRSIG

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000782 Genomic DNA. Translation: AAB91287.1.

PIR

E69546.

RefSeq

NP_071196.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SUW	X-ray	2.45	A/B/C/D	1-249	[»]
1Z0S	X-ray	1.70	A/B/C/D	1-249	[»]
1Z0U	X-ray	2.00	A/B	1-249	[»]
1Z0Z	X-ray	2.85	A/B/C/D	1-249	[»]

ModBase

Search...

Genome annotation databases

GeneID

1485603.

GenomeReviews

Gene locus AF_2373 in contig AE000782_GR.

KEGG

afu:AF2373.

NMPDR

fig|224325.1.peg.2357.

TIGR

AF_2373.

Phylogenomic databases

HOGENOM

O30297.

OMA

IVSTPHG.

Enzyme and pathway databases

BioCyc

AFUL224325:AF_2373-MON.

BRENDA

2.7.1.23. 7576.

Family and domain databases

HAMAP

MF_00361.
[Tree]

InterPro

IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. ATP_NADK.
[Graphical view]

Gene3D

G3DSA:2.60.200.30. ATP-NAD_kinase_PpnK-typ. 1 hit.

PANTHER

PTHR20275. ATP_NADK. 1 hit.

Pfam

PF01513. NAD_kinase. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PPNK_ARCFU

Accession

Primary (citable) accession number: O30297

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)