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Protein

NAD kinase

Gene

nadK

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of the intracellular balance between NAD(H) and NADP(H), and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.1 Publication

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei49 – 491Proton acceptorUniRule annotation
Binding sitei54 – 541NADUniRule annotation1 Publication
Binding sitei126 – 1261NADUniRule annotation1 Publication
Binding sitei143 – 1431NADUniRule annotation1 Publication
Binding sitei145 – 1451NAD1 Publication
Binding sitei153 – 1531NAD; via carbonyl oxygenUniRule annotation1 Publication
Binding sitei180 – 1801NAD; via carbonyl oxygen1 Publication
Binding sitei211 – 2111NADUniRule annotation1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi49 – 502NADUniRule annotation1 Publication
Nucleotide bindingi115 – 1162NADUniRule annotation1 Publication
Nucleotide bindingi156 – 1616NADUniRule annotation1 Publication

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB
  4. NAD binding Source: UniProtKB

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2422-MONOMER.
BRENDAi2.7.1.23. 414.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:AF_2373
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
ProteomesiUP000002199 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249NAD kinasePRO_0000120697Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi224325.AF2373.

Structurei

Secondary structure

1
249
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 109Combined sources
Helixi13 – 2210Combined sources
Beta strandi26 – 327Combined sources
Helixi35 – 395Combined sources
Beta strandi40 – 478Combined sources
Helixi49 – 568Combined sources
Beta strandi65 – 695Combined sources
Beta strandi71 – 733Combined sources
Turni81 – 833Combined sources
Helixi85 – 9410Combined sources
Beta strandi97 – 1004Combined sources
Beta strandi103 – 1053Combined sources
Beta strandi108 – 12316Combined sources
Beta strandi128 – 1347Combined sources
Beta strandi137 – 15216Combined sources
Helixi153 – 1564Combined sources
Helixi158 – 1614Combined sources
Beta strandi174 – 1807Combined sources
Beta strandi183 – 1853Combined sources
Beta strandi189 – 1913Combined sources
Beta strandi197 – 20812Combined sources
Turni209 – 2113Combined sources
Beta strandi212 – 22413Combined sources
Beta strandi229 – 2324Combined sources
Helixi237 – 24610Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SUWX-ray2.45A/B/C/D1-249[»]
1Z0SX-ray1.70A/B/C/D1-249[»]
1Z0UX-ray2.00A/B1-249[»]
1Z0ZX-ray2.85A/B/C/D1-249[»]
SMRiO30297. Positions 1-249.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO30297.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
KOiK00858.
OMAiRIKRITE.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

O30297-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAAVVYKTD GHVKRIEEAL KRLEVEVELF NQPSEELENF DFIVSVGGDG
60 70 80 90 100
TILRILQKLK RCPPIFGINT GRVGLLTHAS PENFEVELKK AVEKFEVERF
110 120 130 140 150
PRVSCSAMPD VLALNEIAVL SRKPAKMIDV ALRVDGVEVD RIRCDGFIVA
160 170 180 190 200
TQIGSTGYAF SAGGPVVEPY LECFILIPIA PFRFGWKPYV VSMERKIEVI
210 220 230 240
AEKAIVVADG QKSVDFDGEI TIEKSEFPAV FFKNEKRFRN LFGKVRSIG
Length:249
Mass (Da):27,868
Last modified:January 1, 1998 - v1
Checksum:iE07E3163BD14FC7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB91287.1.
PIRiE69546.
RefSeqiNP_071196.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB91287; AAB91287; AF_2373.
GeneIDi1485603.
KEGGiafu:AF2373.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB91287.1.
PIRiE69546.
RefSeqiNP_071196.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SUWX-ray2.45A/B/C/D1-249[»]
1Z0SX-ray1.70A/B/C/D1-249[»]
1Z0UX-ray2.00A/B1-249[»]
1Z0ZX-ray2.85A/B/C/D1-249[»]
SMRiO30297. Positions 1-249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF2373.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB91287; AAB91287; AF_2373.
GeneIDi1485603.
KEGGiafu:AF2373.

Phylogenomic databases

eggNOGiCOG0061.
KOiK00858.
OMAiRIKRITE.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2422-MONOMER.
BRENDAi2.7.1.23. 414.

Miscellaneous databases

EvolutionaryTraceiO30297.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
  2. "Crystal structures of an NAD kinase from Archaeoglobus fulgidus in complex with ATP, NAD, or NADP."
    Liu J., Lou Y., Yokota H., Adams P.D., Kim R., Kim S.H.
    J. Mol. Biol. 354:289-303(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) IN COMPLEX WITH ATP AND NAD, FUNCTION, SUBUNIT.

Entry informationi

Entry nameiNADK_ARCFU
AccessioniPrimary (citable) accession number: O30297
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: April 29, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.