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O30297

- NADK_ARCFU

UniProt

O30297 - NADK_ARCFU

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Protein

NAD kinase

Gene
nadK, AF_2373
Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the regulation of the intracellular balance between NAD(H) and NADP(H), and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.1 Publication

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

Divalent metal ions By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei49 – 491Proton acceptor By similarity
Binding sitei54 – 541NAD
Binding sitei126 – 1261NAD
Binding sitei143 – 1431NAD
Binding sitei145 – 1451NAD Inferred
Binding sitei153 – 1531NAD; via carbonyl oxygen
Binding sitei180 – 1801NAD; via carbonyl oxygen Inferred
Binding sitei211 – 2111NAD

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi49 – 502NADUniRule annotation
Nucleotide bindingi115 – 1162NADUniRule annotation
Nucleotide bindingi156 – 1616NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB
  4. NAD binding Source: UniProtKB

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2422-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinase (EC:2.7.1.23)
Alternative name(s):
ATP-dependent NAD kinase
Gene namesi
Name:nadK
Ordered Locus Names:AF_2373
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
ProteomesiUP000002199: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249NAD kinaseUniRule annotationPRO_0000120697Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi224325.AF2373.

Structurei

Secondary structure

1
249
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 109
Helixi13 – 2210
Beta strandi26 – 327
Helixi35 – 395
Beta strandi40 – 478
Helixi49 – 568
Beta strandi65 – 695
Beta strandi71 – 733
Turni81 – 833
Helixi85 – 9410
Beta strandi97 – 1004
Beta strandi103 – 1053
Beta strandi108 – 12316
Beta strandi128 – 1347
Beta strandi137 – 15216
Helixi153 – 1564
Helixi158 – 1614
Beta strandi174 – 1807
Beta strandi183 – 1853
Beta strandi189 – 1913
Beta strandi197 – 20812
Turni209 – 2113
Beta strandi212 – 22413
Beta strandi229 – 2324
Helixi237 – 24610

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SUWX-ray2.45A/B/C/D1-249[»]
1Z0SX-ray1.70A/B/C/D1-249[»]
1Z0UX-ray2.00A/B1-249[»]
1Z0ZX-ray2.85A/B/C/D1-249[»]
ProteinModelPortaliO30297.
SMRiO30297. Positions 1-249.

Miscellaneous databases

EvolutionaryTraceiO30297.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.

Phylogenomic databases

eggNOGiCOG0061.
KOiK00858.
OMAiWREWIAT.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

O30297-1 [UniParc]FASTAAdd to Basket

« Hide

MRAAVVYKTD GHVKRIEEAL KRLEVEVELF NQPSEELENF DFIVSVGGDG    50
TILRILQKLK RCPPIFGINT GRVGLLTHAS PENFEVELKK AVEKFEVERF 100
PRVSCSAMPD VLALNEIAVL SRKPAKMIDV ALRVDGVEVD RIRCDGFIVA 150
TQIGSTGYAF SAGGPVVEPY LECFILIPIA PFRFGWKPYV VSMERKIEVI 200
AEKAIVVADG QKSVDFDGEI TIEKSEFPAV FFKNEKRFRN LFGKVRSIG 249
Length:249
Mass (Da):27,868
Last modified:January 1, 1998 - v1
Checksum:iE07E3163BD14FC7B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000782 Genomic DNA. Translation: AAB91287.1.
PIRiE69546.
RefSeqiNP_071196.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB91287; AAB91287; AF_2373.
GeneIDi1485603.
KEGGiafu:AF2373.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000782 Genomic DNA. Translation: AAB91287.1 .
PIRi E69546.
RefSeqi NP_071196.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1SUW X-ray 2.45 A/B/C/D 1-249 [» ]
1Z0S X-ray 1.70 A/B/C/D 1-249 [» ]
1Z0U X-ray 2.00 A/B 1-249 [» ]
1Z0Z X-ray 2.85 A/B/C/D 1-249 [» ]
ProteinModelPortali O30297.
SMRi O30297. Positions 1-249.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224325.AF2373.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB91287 ; AAB91287 ; AF_2373 .
GeneIDi 1485603.
KEGGi afu:AF2373.

Phylogenomic databases

eggNOGi COG0061.
KOi K00858.
OMAi WREWIAT.

Enzyme and pathway databases

BioCyci AFUL224325:GJBC-2422-MONOMER.

Miscellaneous databases

EvolutionaryTracei O30297.

Family and domain databases

Gene3Di 2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPi MF_00361. NAD_kinase.
InterProi IPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view ]
PANTHERi PTHR20275. PTHR20275. 1 hit.
Pfami PF01513. NAD_kinase. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
  2. "Crystal structures of an NAD kinase from Archaeoglobus fulgidus in complex with ATP, NAD, or NADP."
    Liu J., Lou Y., Yokota H., Adams P.D., Kim R., Kim S.H.
    J. Mol. Biol. 354:289-303(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) IN COMPLEX WITH ATP AND NAD, FUNCTION, SUBUNIT.

Entry informationi

Entry nameiNADK_ARCFU
AccessioniPrimary (citable) accession number: O30297
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3