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Protein

NAD kinase

Gene

nadK

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of the intracellular balance between NAD(H) and NADP(H), and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.1 Publication

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei49Proton acceptorUniRule annotation1
Binding sitei54NADUniRule annotation1 Publication1
Binding sitei126NADUniRule annotation1 Publication1
Binding sitei143NADUniRule annotation1 Publication1
Binding sitei145NAD1 Publication1
Binding sitei153NAD; via carbonyl oxygenUniRule annotation1 Publication1
Binding sitei180NAD; via carbonyl oxygen1 Publication1
Binding sitei211NADUniRule annotation1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi49 – 50NADUniRule annotation1 Publication2
Nucleotide bindingi115 – 116NADUniRule annotation1 Publication2
Nucleotide bindingi156 – 161NADUniRule annotation1 Publication6

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-HAMAP
  • NAD+ kinase activity Source: UniProtKB
  • NAD binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.23. 414.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:AF_2373
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001206971 – 249NAD kinaseAdd BLAST249

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi224325.AF2373.

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 10Combined sources9
Helixi13 – 22Combined sources10
Beta strandi26 – 32Combined sources7
Helixi35 – 39Combined sources5
Beta strandi40 – 47Combined sources8
Helixi49 – 56Combined sources8
Beta strandi65 – 69Combined sources5
Beta strandi71 – 73Combined sources3
Turni81 – 83Combined sources3
Helixi85 – 94Combined sources10
Beta strandi97 – 100Combined sources4
Beta strandi103 – 105Combined sources3
Beta strandi108 – 123Combined sources16
Beta strandi128 – 134Combined sources7
Beta strandi137 – 152Combined sources16
Helixi153 – 156Combined sources4
Helixi158 – 161Combined sources4
Beta strandi174 – 180Combined sources7
Beta strandi183 – 185Combined sources3
Beta strandi189 – 191Combined sources3
Beta strandi197 – 208Combined sources12
Turni209 – 211Combined sources3
Beta strandi212 – 224Combined sources13
Beta strandi229 – 232Combined sources4
Helixi237 – 246Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SUWX-ray2.45A/B/C/D1-249[»]
1Z0SX-ray1.70A/B/C/D1-249[»]
1Z0UX-ray2.00A/B1-249[»]
1Z0ZX-ray2.85A/B/C/D1-249[»]
ProteinModelPortaliO30297.
SMRiO30297.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO30297.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01348. Archaea.
COG0061. LUCA.
KOiK00858.
OMAiGPILHES.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

O30297-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAAVVYKTD GHVKRIEEAL KRLEVEVELF NQPSEELENF DFIVSVGGDG
60 70 80 90 100
TILRILQKLK RCPPIFGINT GRVGLLTHAS PENFEVELKK AVEKFEVERF
110 120 130 140 150
PRVSCSAMPD VLALNEIAVL SRKPAKMIDV ALRVDGVEVD RIRCDGFIVA
160 170 180 190 200
TQIGSTGYAF SAGGPVVEPY LECFILIPIA PFRFGWKPYV VSMERKIEVI
210 220 230 240
AEKAIVVADG QKSVDFDGEI TIEKSEFPAV FFKNEKRFRN LFGKVRSIG
Length:249
Mass (Da):27,868
Last modified:January 1, 1998 - v1
Checksum:iE07E3163BD14FC7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB91287.1.
PIRiE69546.
RefSeqiWP_010879860.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB91287; AAB91287; AF_2373.
GeneIDi1485603.
KEGGiafu:AF_2373.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB91287.1.
PIRiE69546.
RefSeqiWP_010879860.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SUWX-ray2.45A/B/C/D1-249[»]
1Z0SX-ray1.70A/B/C/D1-249[»]
1Z0UX-ray2.00A/B1-249[»]
1Z0ZX-ray2.85A/B/C/D1-249[»]
ProteinModelPortaliO30297.
SMRiO30297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF2373.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB91287; AAB91287; AF_2373.
GeneIDi1485603.
KEGGiafu:AF_2373.

Phylogenomic databases

eggNOGiarCOG01348. Archaea.
COG0061. LUCA.
KOiK00858.
OMAiGPILHES.

Enzyme and pathway databases

BRENDAi2.7.1.23. 414.

Miscellaneous databases

EvolutionaryTraceiO30297.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADK_ARCFU
AccessioniPrimary (citable) accession number: O30297
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.