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O30297

- NADK_ARCFU

UniProt

O30297 - NADK_ARCFU

Protein

NAD kinase

Gene

nadK

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Involved in the regulation of the intracellular balance between NAD(H) and NADP(H), and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.1 Publication

    Catalytic activityi

    ATP + NAD+ = ADP + NADP+.UniRule annotation

    Cofactori

    Divalent metal ions.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei49 – 491Proton acceptorUniRule annotation
    Binding sitei54 – 541NAD1 PublicationUniRule annotation
    Binding sitei126 – 1261NAD1 PublicationUniRule annotation
    Binding sitei143 – 1431NAD1 PublicationUniRule annotation
    Binding sitei145 – 1451NAD1 Publication
    Binding sitei153 – 1531NAD; via carbonyl oxygen1 PublicationUniRule annotation
    Binding sitei180 – 1801NAD; via carbonyl oxygen1 Publication
    Binding sitei211 – 2111NAD1 PublicationUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi49 – 502NAD1 PublicationUniRule annotation
    Nucleotide bindingi115 – 1162NAD1 PublicationUniRule annotation
    Nucleotide bindingi156 – 1616NAD1 PublicationUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. metal ion binding Source: UniProtKB-HAMAP
    3. NAD+ kinase activity Source: UniProtKB
    4. NAD binding Source: UniProtKB

    GO - Biological processi

    1. NAD metabolic process Source: InterPro
    2. NADP biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, NAD, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAFUL224325:GJBC-2422-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
    Alternative name(s):
    ATP-dependent NAD kinaseUniRule annotation
    Gene namesi
    Name:nadKUniRule annotation
    Ordered Locus Names:AF_2373
    OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
    Taxonomic identifieri224325 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
    ProteomesiUP000002199: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 249249NAD kinasePRO_0000120697Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi224325.AF2373.

    Structurei

    Secondary structure

    1
    249
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 109
    Helixi13 – 2210
    Beta strandi26 – 327
    Helixi35 – 395
    Beta strandi40 – 478
    Helixi49 – 568
    Beta strandi65 – 695
    Beta strandi71 – 733
    Turni81 – 833
    Helixi85 – 9410
    Beta strandi97 – 1004
    Beta strandi103 – 1053
    Beta strandi108 – 12316
    Beta strandi128 – 1347
    Beta strandi137 – 15216
    Helixi153 – 1564
    Helixi158 – 1614
    Beta strandi174 – 1807
    Beta strandi183 – 1853
    Beta strandi189 – 1913
    Beta strandi197 – 20812
    Turni209 – 2113
    Beta strandi212 – 22413
    Beta strandi229 – 2324
    Helixi237 – 24610

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SUWX-ray2.45A/B/C/D1-249[»]
    1Z0SX-ray1.70A/B/C/D1-249[»]
    1Z0UX-ray2.00A/B1-249[»]
    1Z0ZX-ray2.85A/B/C/D1-249[»]
    ProteinModelPortaliO30297.
    SMRiO30297. Positions 1-249.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO30297.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0061.
    KOiK00858.
    OMAiWREWIAT.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 1 hit.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O30297-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRAAVVYKTD GHVKRIEEAL KRLEVEVELF NQPSEELENF DFIVSVGGDG    50
    TILRILQKLK RCPPIFGINT GRVGLLTHAS PENFEVELKK AVEKFEVERF 100
    PRVSCSAMPD VLALNEIAVL SRKPAKMIDV ALRVDGVEVD RIRCDGFIVA 150
    TQIGSTGYAF SAGGPVVEPY LECFILIPIA PFRFGWKPYV VSMERKIEVI 200
    AEKAIVVADG QKSVDFDGEI TIEKSEFPAV FFKNEKRFRN LFGKVRSIG 249
    Length:249
    Mass (Da):27,868
    Last modified:January 1, 1998 - v1
    Checksum:iE07E3163BD14FC7B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000782 Genomic DNA. Translation: AAB91287.1.
    PIRiE69546.
    RefSeqiNP_071196.1. NC_000917.1.

    Genome annotation databases

    EnsemblBacteriaiAAB91287; AAB91287; AF_2373.
    GeneIDi1485603.
    KEGGiafu:AF2373.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000782 Genomic DNA. Translation: AAB91287.1 .
    PIRi E69546.
    RefSeqi NP_071196.1. NC_000917.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SUW X-ray 2.45 A/B/C/D 1-249 [» ]
    1Z0S X-ray 1.70 A/B/C/D 1-249 [» ]
    1Z0U X-ray 2.00 A/B 1-249 [» ]
    1Z0Z X-ray 2.85 A/B/C/D 1-249 [» ]
    ProteinModelPortali O30297.
    SMRi O30297. Positions 1-249.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224325.AF2373.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB91287 ; AAB91287 ; AF_2373 .
    GeneIDi 1485603.
    KEGGi afu:AF2373.

    Phylogenomic databases

    eggNOGi COG0061.
    KOi K00858.
    OMAi WREWIAT.

    Enzyme and pathway databases

    BioCyci AFUL224325:GJBC-2422-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O30297.

    Family and domain databases

    Gene3Di 2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPi MF_00361. NAD_kinase.
    InterProi IPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view ]
    PANTHERi PTHR20275. PTHR20275. 1 hit.
    Pfami PF01513. NAD_kinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
      Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
      , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
      Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
    2. "Crystal structures of an NAD kinase from Archaeoglobus fulgidus in complex with ATP, NAD, or NADP."
      Liu J., Lou Y., Yokota H., Adams P.D., Kim R., Kim S.H.
      J. Mol. Biol. 354:289-303(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) IN COMPLEX WITH ATP AND NAD, FUNCTION, SUBUNIT.

    Entry informationi

    Entry nameiNADK_ARCFU
    AccessioniPrimary (citable) accession number: O30297
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3