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Protein

RIO-type serine/threonine-protein kinase Rio2

Gene

rio2

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+, Mn2+Note: Mg2+. Mn2+ is seen in the crystal structure; there are 2 Mn2+ ions in the ATP-bound crystal and 1 Mn2+ in the ADP-bound crystal.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi103Magnesium 11 Publication1
Binding sitei120ATP1 Publication1
Binding sitei126ATPCombined sources1 Publication1
Active sitei218Proton acceptorPROSITE-ProRule annotation1
Binding sitei222ATP; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi223Magnesium 21 Publication1
Binding sitei223ATPCombined sources1 Publication1
Metal bindingi235Magnesium 11 Publication1
Metal bindingi235Magnesium 21 Publication1
Binding sitei235ATPCombined sources2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi98 – 106ATPPROSITE-ProRule annotation9
Nucleotide bindingi180 – 186ATPCombined sources2 Publications7

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 414.

Names & Taxonomyi

Protein namesi
Recommended name:
RIO-type serine/threonine-protein kinase Rio2 (EC:2.7.11.1)
Short name:
AfRio2
Gene namesi
Name:rio2
Ordered Locus Names:AF_2426
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003473341 – 282RIO-type serine/threonine-protein kinase Rio2Add BLAST282

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei128Phosphoserine; by autocatalysis1 Publication1

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiO30245.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi224325.AF2426.

Structurei

Secondary structure

1282
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 8Combined sources6
Helixi12 – 22Combined sources11
Turni23 – 27Combined sources5
Beta strandi29 – 32Combined sources4
Helixi33 – 40Combined sources8
Helixi44 – 56Combined sources13
Beta strandi59 – 63Combined sources5
Beta strandi65 – 67Combined sources3
Beta strandi69 – 72Combined sources4
Helixi74 – 87Combined sources14
Beta strandi92 – 100Combined sources9
Beta strandi102 – 111Combined sources10
Turni112 – 114Combined sources3
Beta strandi115 – 122Combined sources8
Helixi143 – 160Combined sources18
Turni161 – 163Combined sources3
Beta strandi164 – 166Combined sources3
Beta strandi169 – 173Combined sources5
Beta strandi176 – 180Combined sources5
Helixi187 – 189Combined sources3
Helixi195 – 211Combined sources17
Beta strandi223 – 227Combined sources5
Beta strandi230 – 233Combined sources4
Helixi247 – 266Combined sources20
Helixi272 – 280Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TQIX-ray2.00A1-282[»]
1TQMX-ray1.99A1-282[»]
1TQPX-ray2.10A1-282[»]
1ZAOX-ray1.84A1-282[»]
1ZARX-ray1.75A1-282[»]
ProteinModelPortaliO30245.
SMRiO30245.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO30245.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini92 – 282Protein kinasePROSITE-ProRule annotationAdd BLAST191

Sequence similaritiesi

Contains 1 protein kinase domain.Curated

Phylogenomic databases

eggNOGiarCOG01181. Archaea.
COG0478. LUCA.
KOiK07179.
OMAiFITHDER.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR030484. Rio2.
IPR015285. RIO2_wHTH_N.
IPR000687. RIO_kinase.
IPR008266. Tyr_kinase_AS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10593:SF36. PTHR10593:SF36. 1 hit.
PfamiPF09202. Rio2_N. 1 hit.
[Graphical view]
SMARTiSM00090. RIO. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

O30245-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIAELYGKM GKHSWRIMDA IFKNLWDYEY VPLQLISSHA RIGEEKARNI
60 70 80 90 100
LKYLSDLRVV QNRQKDYEGS TFTFIGLSLY SLHRLVRSGK VDAIGKLMGE
110 120 130 140 150
GKESAVFNCY SEKFGECVVK FHKVGHTSFK KVKEKRDYGD LHFSVLAIRS
160 170 180 190 200
ARNEFRALQK LQGLAVPKVY AWEGNAVLME LIDAKELYRV RVENPDEVLD
210 220 230 240 250
MILEEVAKFY HRGIVHGDLS QYNVLVSEEG IWIIDFPQSV EVGEEGWREI
260 270 280
LERDVRNIIT YFSRTYRTEK DINSAIDRIL QE
Length:282
Mass (Da):32,809
Last modified:January 1, 1998 - v1
Checksum:i7DE69AF0EF8DB24D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB91236.1.
PIRiC69553.
RefSeqiWP_010879913.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB91236; AAB91236; AF_2426.
GeneIDi24796150.
KEGGiafu:AF_2426.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB91236.1.
PIRiC69553.
RefSeqiWP_010879913.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TQIX-ray2.00A1-282[»]
1TQMX-ray1.99A1-282[»]
1TQPX-ray2.10A1-282[»]
1ZAOX-ray1.84A1-282[»]
1ZARX-ray1.75A1-282[»]
ProteinModelPortaliO30245.
SMRiO30245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF2426.

PTM databases

iPTMnetiO30245.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB91236; AAB91236; AF_2426.
GeneIDi24796150.
KEGGiafu:AF_2426.

Phylogenomic databases

eggNOGiarCOG01181. Archaea.
COG0478. LUCA.
KOiK07179.
OMAiFITHDER.

Enzyme and pathway databases

BRENDAi2.7.11.1. 414.

Miscellaneous databases

EvolutionaryTraceiO30245.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR030484. Rio2.
IPR015285. RIO2_wHTH_N.
IPR000687. RIO_kinase.
IPR008266. Tyr_kinase_AS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10593:SF36. PTHR10593:SF36. 1 hit.
PfamiPF09202. Rio2_N. 1 hit.
[Graphical view]
SMARTiSM00090. RIO. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRIO2_ARCFU
AccessioniPrimary (citable) accession number: O30245
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.