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O30245

- RIO2_ARCFU

UniProt

O30245 - RIO2_ARCFU

Protein

RIO-type serine/threonine-protein kinase Rio2

Gene

rio2

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium. Mangenese is seen in the crystal structure; there are 2 Mn2+ ions in the ATP-bound crystal and 1 Mn2+ in the ADP-bound crystal.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi103 – 1031Magnesium 1
    Binding sitei120 – 1201ATP
    Binding sitei126 – 1261ATP
    Active sitei218 – 2181Proton acceptorBy similarity
    Metal bindingi223 – 2231Magnesium 2
    Metal bindingi235 – 2351Magnesium 1
    Metal bindingi235 – 2351Magnesium 2
    Binding sitei235 – 2351ATP

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi99 – 1046ATP
    Nucleotide bindingi180 – 1867ATP
    Nucleotide bindingi222 – 2232ATP

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein serine/threonine kinase activity Source: UniProtKB-KW
    4. protein tyrosine kinase activity Source: InterPro

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAFUL224325:GJBC-2478-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RIO-type serine/threonine-protein kinase Rio2 (EC:2.7.11.1)
    Short name:
    AfRio2
    Gene namesi
    Name:rio2
    Ordered Locus Names:AF_2426
    OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
    Taxonomic identifieri224325 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
    ProteomesiUP000002199: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 282282RIO-type serine/threonine-protein kinase Rio2PRO_0000347334Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei128 – 1281Phosphoserine; by autocatalysis1 Publication

    Post-translational modificationi

    Autophosphorylated.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi224325.AF2426.

    Structurei

    Secondary structure

    1
    282
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 86
    Helixi12 – 2211
    Turni23 – 275
    Beta strandi29 – 324
    Helixi33 – 408
    Helixi44 – 5613
    Beta strandi59 – 635
    Beta strandi65 – 673
    Beta strandi69 – 724
    Helixi74 – 8714
    Beta strandi92 – 1009
    Beta strandi102 – 11110
    Turni112 – 1143
    Beta strandi115 – 1228
    Helixi143 – 16018
    Turni161 – 1633
    Beta strandi164 – 1663
    Beta strandi169 – 1735
    Beta strandi176 – 1805
    Helixi187 – 1893
    Helixi195 – 21117
    Beta strandi223 – 2275
    Beta strandi230 – 2334
    Helixi247 – 26620
    Helixi272 – 2809

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1TQIX-ray2.00A1-282[»]
    1TQMX-ray1.99A1-282[»]
    1TQPX-ray2.10A1-282[»]
    1ZAOX-ray1.84A1-282[»]
    1ZARX-ray1.75A1-282[»]
    ProteinModelPortaliO30245.
    SMRiO30245. Positions 1-282.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO30245.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini94 – 236143Protein kinaseAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.Curated

    Phylogenomic databases

    eggNOGiCOG0478.
    KOiK07179.
    OMAiSHARIGE.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR018934. RIO-like_kinase.
    IPR015285. RIO2_kinase_winged_hlx_N.
    IPR000687. RIO_kinase.
    IPR008266. Tyr_kinase_AS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF01163. RIO1. 1 hit.
    PF09202. Rio2_N. 1 hit.
    [Graphical view]
    SMARTiSM00090. RIO. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O30245-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNIAELYGKM GKHSWRIMDA IFKNLWDYEY VPLQLISSHA RIGEEKARNI    50
    LKYLSDLRVV QNRQKDYEGS TFTFIGLSLY SLHRLVRSGK VDAIGKLMGE 100
    GKESAVFNCY SEKFGECVVK FHKVGHTSFK KVKEKRDYGD LHFSVLAIRS 150
    ARNEFRALQK LQGLAVPKVY AWEGNAVLME LIDAKELYRV RVENPDEVLD 200
    MILEEVAKFY HRGIVHGDLS QYNVLVSEEG IWIIDFPQSV EVGEEGWREI 250
    LERDVRNIIT YFSRTYRTEK DINSAIDRIL QE 282
    Length:282
    Mass (Da):32,809
    Last modified:January 1, 1998 - v1
    Checksum:i7DE69AF0EF8DB24D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000782 Genomic DNA. Translation: AAB91236.1.
    PIRiC69553.
    RefSeqiNP_071248.1. NC_000917.1.

    Genome annotation databases

    EnsemblBacteriaiAAB91236; AAB91236; AF_2426.
    GeneIDi1485657.
    KEGGiafu:AF2426.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000782 Genomic DNA. Translation: AAB91236.1 .
    PIRi C69553.
    RefSeqi NP_071248.1. NC_000917.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1TQI X-ray 2.00 A 1-282 [» ]
    1TQM X-ray 1.99 A 1-282 [» ]
    1TQP X-ray 2.10 A 1-282 [» ]
    1ZAO X-ray 1.84 A 1-282 [» ]
    1ZAR X-ray 1.75 A 1-282 [» ]
    ProteinModelPortali O30245.
    SMRi O30245. Positions 1-282.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224325.AF2426.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB91236 ; AAB91236 ; AF_2426 .
    GeneIDi 1485657.
    KEGGi afu:AF2426.

    Phylogenomic databases

    eggNOGi COG0478.
    KOi K07179.
    OMAi SHARIGE.

    Enzyme and pathway databases

    BioCyci AFUL224325:GJBC-2478-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O30245.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR018934. RIO-like_kinase.
    IPR015285. RIO2_kinase_winged_hlx_N.
    IPR000687. RIO_kinase.
    IPR008266. Tyr_kinase_AS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF01163. RIO1. 1 hit.
    PF09202. Rio2_N. 1 hit.
    [Graphical view ]
    SMARTi SM00090. RIO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
      Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
      , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
      Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
    2. "Crystal structure of A. fulgidus Rio2 defines a new family of serine protein kinases."
      LaRonde-LeBlanc N., Wlodawer A.
      Structure 12:1585-1594(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) OF APOPROTEIN AND IN COMPLEXES WITH ATP AND ATP ANALOG, FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
      Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
    3. "Autophosphorylation of Archaeoglobus fulgidus Rio2 and crystal structures of its nucleotide-metal ion complexes."
      LaRonde-LeBlanc N., Guszczynski T., Copeland T., Wlodawer A.
      FEBS J. 272:2800-2810(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS) IN COMPLEXES WITH MN-ATP AND MN-ADP, PHOSPHORYLATION AT SER-128.
      Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

    Entry informationi

    Entry nameiRIO2_ARCFU
    AccessioniPrimary (citable) accession number: O30245
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3