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O30245 (RIO2_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RIO-type serine/threonine-protein kinase Rio2

Short name=AfRio2
EC=2.7.11.1
Gene names
Name:rio2
Ordered Locus Names:AF_2426
OrganismArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) [Reference proteome] [HAMAP]
Taxonomic identifier224325 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium. Mangenese is seen in the crystal structure; there are 2 Mn2+ ions in the ATP-bound crystal and 1 Mn2+ in the ADP-bound crystal.

Subunit structure

Monomer. Ref.2

Post-translational modification

Autophosphorylated. Ref.3

Sequence similarities

Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 282282RIO-type serine/threonine-protein kinase Rio2
PRO_0000347334

Regions

Domain94 – 236143Protein kinase
Nucleotide binding99 – 1046ATP
Nucleotide binding180 – 1867ATP
Nucleotide binding222 – 2232ATP

Sites

Active site2181Proton acceptor By similarity
Metal binding1031Magnesium 1
Metal binding2231Magnesium 2
Metal binding2351Magnesium 1
Metal binding2351Magnesium 2
Binding site1201ATP
Binding site1261ATP
Binding site2351ATP

Amino acid modifications

Modified residue1281Phosphoserine; by autocatalysis Ref.3

Secondary structure

............................................. 282
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O30245 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 7DE69AF0EF8DB24D

FASTA28232,809
        10         20         30         40         50         60 
MNIAELYGKM GKHSWRIMDA IFKNLWDYEY VPLQLISSHA RIGEEKARNI LKYLSDLRVV 

        70         80         90        100        110        120 
QNRQKDYEGS TFTFIGLSLY SLHRLVRSGK VDAIGKLMGE GKESAVFNCY SEKFGECVVK 

       130        140        150        160        170        180 
FHKVGHTSFK KVKEKRDYGD LHFSVLAIRS ARNEFRALQK LQGLAVPKVY AWEGNAVLME 

       190        200        210        220        230        240 
LIDAKELYRV RVENPDEVLD MILEEVAKFY HRGIVHGDLS QYNVLVSEEG IWIIDFPQSV 

       250        260        270        280 
EVGEEGWREI LERDVRNIIT YFSRTYRTEK DINSAIDRIL QE 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. expand/collapse author list , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
[2]"Crystal structure of A. fulgidus Rio2 defines a new family of serine protein kinases."
LaRonde-LeBlanc N., Wlodawer A.
Structure 12:1585-1594(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) OF APOPROTEIN AND IN COMPLEXES WITH ATP AND ATP ANALOG, FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
[3]"Autophosphorylation of Archaeoglobus fulgidus Rio2 and crystal structures of its nucleotide-metal ion complexes."
LaRonde-LeBlanc N., Guszczynski T., Copeland T., Wlodawer A.
FEBS J. 272:2800-2810(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS) IN COMPLEXES WITH MN-ATP AND MN-ADP, PHOSPHORYLATION AT SER-128.
Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000782 Genomic DNA. Translation: AAB91236.1.
PIRC69553.
RefSeqNP_071248.1. NC_000917.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1TQIX-ray2.00A1-282[»]
1TQMX-ray1.99A1-282[»]
1TQPX-ray2.10A1-282[»]
1ZAOX-ray1.84A1-282[»]
1ZARX-ray1.75A1-282[»]
ProteinModelPortalO30245.
SMRO30245. Positions 1-282.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224325.AF2426.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB91236; AAB91236; AF_2426.
GeneID1485657.
KEGGafu:AF2426.

Phylogenomic databases

eggNOGCOG0478.
KOK07179.
OMAKYHRIGR.
ProtClustDBCLSK2747315.

Enzyme and pathway databases

BioCycAFUL224325:GJBC-2478-MONOMER.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR011009. Kinase-like_dom.
IPR018934. RIO-like_kinase.
IPR015285. RIO2_kinase_winged_hlx_N.
IPR000687. RIO_kinase.
IPR008266. Tyr_kinase_AS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF01163. RIO1. 1 hit.
PF09202. Rio2_N. 1 hit.
[Graphical view]
SMARTSM00090. RIO. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceO30245.

Entry information

Entry nameRIO2_ARCFU
AccessionPrimary (citable) accession number: O30245
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references