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O30245

- RIO2_ARCFU

UniProt

O30245 - RIO2_ARCFU

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Protein

RIO-type serine/threonine-protein kinase Rio2

Gene

rio2

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium. Mangenese is seen in the crystal structure; there are 2 Mn2+ ions in the ATP-bound crystal and 1 Mn2+ in the ADP-bound crystal.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi103 – 1031Magnesium 1
Binding sitei120 – 1201ATP
Binding sitei126 – 1261ATP
Active sitei218 – 2181Proton acceptorBy similarity
Metal bindingi223 – 2231Magnesium 2
Metal bindingi235 – 2351Magnesium 1
Metal bindingi235 – 2351Magnesium 2
Binding sitei235 – 2351ATP

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi99 – 1046ATP
Nucleotide bindingi180 – 1867ATP
Nucleotide bindingi222 – 2232ATP

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2478-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RIO-type serine/threonine-protein kinase Rio2 (EC:2.7.11.1)
Short name:
AfRio2
Gene namesi
Name:rio2
Ordered Locus Names:AF_2426
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
ProteomesiUP000002199: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282RIO-type serine/threonine-protein kinase Rio2PRO_0000347334Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine; by autocatalysis1 Publication

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi224325.AF2426.

Structurei

Secondary structure

1
282
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 86
Helixi12 – 2211
Turni23 – 275
Beta strandi29 – 324
Helixi33 – 408
Helixi44 – 5613
Beta strandi59 – 635
Beta strandi65 – 673
Beta strandi69 – 724
Helixi74 – 8714
Beta strandi92 – 1009
Beta strandi102 – 11110
Turni112 – 1143
Beta strandi115 – 1228
Helixi143 – 16018
Turni161 – 1633
Beta strandi164 – 1663
Beta strandi169 – 1735
Beta strandi176 – 1805
Helixi187 – 1893
Helixi195 – 21117
Beta strandi223 – 2275
Beta strandi230 – 2334
Helixi247 – 26620
Helixi272 – 2809

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TQIX-ray2.00A1-282[»]
1TQMX-ray1.99A1-282[»]
1TQPX-ray2.10A1-282[»]
1ZAOX-ray1.84A1-282[»]
1ZARX-ray1.75A1-282[»]
ProteinModelPortaliO30245.
SMRiO30245. Positions 1-282.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO30245.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini94 – 236143Protein kinaseAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.Curated

Phylogenomic databases

eggNOGiCOG0478.
KOiK07179.
OMAiSHARIGE.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR018934. RIO-like_kinase.
IPR015285. RIO2_kinase_winged_hlx_N.
IPR000687. RIO_kinase.
IPR008266. Tyr_kinase_AS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01163. RIO1. 1 hit.
PF09202. Rio2_N. 1 hit.
[Graphical view]
SMARTiSM00090. RIO. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

O30245 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNIAELYGKM GKHSWRIMDA IFKNLWDYEY VPLQLISSHA RIGEEKARNI
60 70 80 90 100
LKYLSDLRVV QNRQKDYEGS TFTFIGLSLY SLHRLVRSGK VDAIGKLMGE
110 120 130 140 150
GKESAVFNCY SEKFGECVVK FHKVGHTSFK KVKEKRDYGD LHFSVLAIRS
160 170 180 190 200
ARNEFRALQK LQGLAVPKVY AWEGNAVLME LIDAKELYRV RVENPDEVLD
210 220 230 240 250
MILEEVAKFY HRGIVHGDLS QYNVLVSEEG IWIIDFPQSV EVGEEGWREI
260 270 280
LERDVRNIIT YFSRTYRTEK DINSAIDRIL QE
Length:282
Mass (Da):32,809
Last modified:January 1, 1998 - v1
Checksum:i7DE69AF0EF8DB24D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000782 Genomic DNA. Translation: AAB91236.1.
PIRiC69553.
RefSeqiNP_071248.1. NC_000917.1.
WP_010879913.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB91236; AAB91236; AF_2426.
GeneIDi1485657.
KEGGiafu:AF2426.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000782 Genomic DNA. Translation: AAB91236.1 .
PIRi C69553.
RefSeqi NP_071248.1. NC_000917.1.
WP_010879913.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1TQI X-ray 2.00 A 1-282 [» ]
1TQM X-ray 1.99 A 1-282 [» ]
1TQP X-ray 2.10 A 1-282 [» ]
1ZAO X-ray 1.84 A 1-282 [» ]
1ZAR X-ray 1.75 A 1-282 [» ]
ProteinModelPortali O30245.
SMRi O30245. Positions 1-282.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224325.AF2426.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB91236 ; AAB91236 ; AF_2426 .
GeneIDi 1485657.
KEGGi afu:AF2426.

Phylogenomic databases

eggNOGi COG0478.
KOi K07179.
OMAi SHARIGE.

Enzyme and pathway databases

BioCyci AFUL224325:GJBC-2478-MONOMER.

Miscellaneous databases

EvolutionaryTracei O30245.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR011009. Kinase-like_dom.
IPR018934. RIO-like_kinase.
IPR015285. RIO2_kinase_winged_hlx_N.
IPR000687. RIO_kinase.
IPR008266. Tyr_kinase_AS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF01163. RIO1. 1 hit.
PF09202. Rio2_N. 1 hit.
[Graphical view ]
SMARTi SM00090. RIO. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
  2. "Crystal structure of A. fulgidus Rio2 defines a new family of serine protein kinases."
    LaRonde-LeBlanc N., Wlodawer A.
    Structure 12:1585-1594(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) OF APOPROTEIN AND IN COMPLEXES WITH ATP AND ATP ANALOG, FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
  3. "Autophosphorylation of Archaeoglobus fulgidus Rio2 and crystal structures of its nucleotide-metal ion complexes."
    LaRonde-LeBlanc N., Guszczynski T., Copeland T., Wlodawer A.
    FEBS J. 272:2800-2810(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS) IN COMPLEXES WITH MN-ATP AND MN-ADP, PHOSPHORYLATION AT SER-128.
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Entry informationi

Entry nameiRIO2_ARCFU
AccessioniPrimary (citable) accession number: O30245
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3