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Protein

O-phospho-L-seryl-tRNA:Cys-tRNA synthase 1

Gene

AF_0028

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)).1 Publication

Catalytic activityi

O-phospho-L-seryl-tRNA(Cys) + sulfide = L-cysteinyl-tRNA(Cys) + phosphate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79Sulfur donor or Sep-tRNA(Cys)1
Binding sitei103Sulfur donor or Sep-tRNA(Cys)1
Binding sitei104Sulfur donor or Sep-tRNA(Cys)1
Binding sitei183Pyridoxal phosphate1
Binding sitei267Sulfur donor or Sep-tRNA(Cys)1
Binding sitei270Sulfur donor or Sep-tRNA(Cys)1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi2.5.1.73. 414.

Names & Taxonomyi

Protein namesi
Recommended name:
O-phospho-L-seryl-tRNA:Cys-tRNA synthase 1 (EC:2.5.1.73)
Alternative name(s):
Sep-tRNA:Cys-tRNA synthase 1
Short name:
SepCysS 1
Gene namesi
Ordered Locus Names:AF_0028
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001074771 – 371O-phospho-L-seryl-tRNA:Cys-tRNA synthase 1Add BLAST371

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei209N6-(pyridoxal phosphate)lysine1

Interactioni

Subunit structurei

Homodimer. Probably interacts with SepRS.1 Publication

Protein-protein interaction databases

STRINGi224325.AF0028.

Structurei

Secondary structure

1371
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 17Combined sources4
Helixi23 – 31Combined sources9
Helixi55 – 60Combined sources6
Helixi62 – 66Combined sources5
Beta strandi69 – 77Combined sources9
Helixi78 – 89Combined sources12
Beta strandi95 – 99Combined sources5
Helixi104 – 112Combined sources9
Beta strandi116 – 120Combined sources5
Turni125 – 127Combined sources3
Helixi132 – 142Combined sources11
Turni143 – 145Combined sources3
Beta strandi148 – 156Combined sources9
Turni158 – 160Combined sources3
Helixi166 – 174Combined sources9
Turni175 – 177Combined sources3
Beta strandi180 – 183Combined sources4
Turni185 – 187Combined sources3
Helixi195 – 198Combined sources4
Beta strandi201 – 206Combined sources6
Helixi207 – 210Combined sources4
Beta strandi218 – 222Combined sources5
Turni224 – 231Combined sources8
Helixi242 – 244Combined sources3
Helixi252 – 267Combined sources16
Helixi268 – 270Combined sources3
Helixi271 – 287Combined sources17
Beta strandi291 – 297Combined sources7
Beta strandi300 – 306Combined sources7
Helixi308 – 316Combined sources9
Beta strandi317 – 319Combined sources3
Helixi320 – 322Combined sources3
Helixi323 – 330Combined sources8
Beta strandi342 – 347Combined sources6
Helixi353 – 369Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E7IX-ray3.00A/B1-371[»]
2E7JX-ray2.40A/B1-371[»]
ProteinModelPortaliO30207.
SMRiO30207.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO30207.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 79Pyridoxal phosphate binding2
Regioni206 – 208Pyridoxal phosphate binding3

Sequence similaritiesi

Belongs to the SepCysS family.Curated

Phylogenomic databases

eggNOGiarCOG00091. Archaea.
COG1103. LUCA.
KOiK06868.
OMAiWDGYSVC.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
HAMAPiMF_01675. Sep_Cys_tRNA_synth. 1 hit.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR013375. Sep_Cys-tRNA_synth_arc.
IPR008829. SepSecS/SepCysS.
[Graphical view]
PfamiPF05889. SepSecS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02539. SepCysS. 1 hit.

Sequencei

Sequence statusi: Complete.

O30207-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKRETKDFI NIDPLQTGGK LTEEARQALL EWGDGYSVCD FCTTGRLDEI
60 70 80 90 100
KTPPIHDFIH NQLPKFLGCD VARVTNGARE AKFAVMHSLA KKDAWVVMDE
110 120 130 140 150
NCHYSSYVAA ERAGLNIALV PKTDYPDYAI TPENFAQTIE ETKKRGEVVL
160 170 180 190 200
ALITYPDGNY GNLPDVKKIA KVCSEYDVPL LVNGAYAIGR MPVSLKEIGA
210 220 230 240 250
DFIVGSGHKS MAASGPIGVM GMKEEWAEIV LRRSEKYKNK EVELLGCTAR
260 270 280 290 300
GATIITLMAS FPHVRERIKR WDEEVEKARR FAAEMEKLGI KQLGDNPHNH
310 320 330 340 350
DLMFFHAEVL YEISKKAKGG RFFLYRELKS RKIHGIKPGL TRYFKLSTYG
360 370
LSDEEVDYVL NAFKEIIEKY S
Length:371
Mass (Da):41,930
Last modified:January 1, 1998 - v1
Checksum:iB22056D00437BEAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB91200.1.
PIRiD69253.
RefSeqiWP_010877542.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB91200; AAB91200; AF_0028.
GeneIDi1483238.
KEGGiafu:AF_0028.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB91200.1.
PIRiD69253.
RefSeqiWP_010877542.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E7IX-ray3.00A/B1-371[»]
2E7JX-ray2.40A/B1-371[»]
ProteinModelPortaliO30207.
SMRiO30207.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF0028.

Protocols and materials databases

DNASUi1483238.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB91200; AAB91200; AF_0028.
GeneIDi1483238.
KEGGiafu:AF_0028.

Phylogenomic databases

eggNOGiarCOG00091. Archaea.
COG1103. LUCA.
KOiK06868.
OMAiWDGYSVC.

Enzyme and pathway databases

BRENDAi2.5.1.73. 414.

Miscellaneous databases

EvolutionaryTraceiO30207.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
HAMAPiMF_01675. Sep_Cys_tRNA_synth. 1 hit.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR013375. Sep_Cys-tRNA_synth_arc.
IPR008829. SepSecS/SepCysS.
[Graphical view]
PfamiPF05889. SepSecS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02539. SepCysS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSPSS1_ARCFU
AccessioniPrimary (citable) accession number: O30207
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.