O30142 (WTPA_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Molybdate/tungstate-binding protein wtpA

Gene names

Name:	wtpA
Synonyms:	modA
Ordered Locus Names:	AF_0094

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the ABC transporter complex wtpABC involved in molybdate/tungstate import. Binds tungstate and molybdate.
Subunit structure	The complex is composed of two ATP-binding proteins (wtpC), two transmembrane proteins (wtpB) and a solute-binding protein (wtpA). Ref.2
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the bacterial solute-binding protein 1 family. WtpA subfamily.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Metal-binding Molybdenum
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Potential

Chain

25 – 342

318

Molybdate/tungstate-binding protein wtpA

PRO_0000159717

Sites

Metal binding

41

1

Molybdate or tungstate; via amide nitrogen

Metal binding

42

1

Molybdate or tungstate

Metal binding

70

1

Molybdate or tungstate

Metal binding

153

1

Molybdate or tungstate

Metal binding

155

1

Molybdate or tungstate; via amide nitrogen

Metal binding

218

1

Molybdate or tungstate

Metal binding

236

1

Molybdate or tungstate

Secondary structure

1

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342

Helix Strand Turn

Details...

Beta strand

33 – 40

8

Helix

41 – 43

3

Helix

44 – 57

14

Beta strand

61 – 68

8

Helix

70 – 78

9

Beta strand

85 – 91

7

Helix

92 – 99

8

Turn

100 – 103

4

Beta strand

108 – 112

5

Beta strand

115 – 119

5

Turn

124 – 128

5

Turn

131 – 133

3

Helix

134 – 138

5

Beta strand

145 – 148

4

Turn

150 – 152

3

Helix

154 – 169

16

Helix

175 – 179

5

Helix

181 – 183

3

Beta strand

188 – 191

4

Beta strand

194 – 198

5

Helix

202 – 204

3

Turn

209 – 211

3

Beta strand

212 – 217

6

Helix

218 – 220

3

Helix

221 – 226

6

Beta strand

231 – 236

6

Helix

237 – 242

6

Beta strand

246 – 249

4

Turn

252 – 254

3

Helix

259 – 261

3

Helix

262 – 265

4

Beta strand

268 – 271

4

Beta strand

277 – 279

3

Beta strand

284 – 288

5

Helix

296 – 307

12

Helix

309 – 317

9

Beta strand

322 – 330

9

Helix

333 – 335

3

Turn

336 – 338

3

Sequences

	Sequence		Length	Mass (Da)
	O30142 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 2DACAF169A074406	FASTA	342	38,630
	10 20 30 40 50 60 MHIGGGVVKI RILILLMLAL FLLGCSSNVN TNVKLKVFHA GSLTEPMKAF KRAFEEKHPN 70 80 90 100 110 120 VEVQTEAAGS AATIRKVTEL GRKADVIATA DYTLIQKMMY PEFANWTIMF AKNQIVLAYR 130 140 150 160 170 180 NDSRYADEIN SQNWYEILKR PDVRFGFSNP NDDPCGYRSL MAIQLAELYY NDPTIFDELV 190 200 210 220 230 240 AKNSNLRFSE DNGSYVLRMP SSERIEINKS KIMIRSMEME LIHLVESGEL DYFFIYKSVA 250 260 270 280 290 300 KQHGFNFVEL PVEIDLSSPD YAELYSKVKV VLANGKEVTG KPIVYGITIP KNAENRELAV 310 320 330 340 EFVKLVISEE GQEILRELGQ EPLVPPRADT AVPSLKAMVE VS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
[2]	"Structure of an ABC transporter in complex with its binding protein." Hollenstein K., Frei D.C., Locher K.P. Nature 446:213-216(2007) [PubMed: 17322901] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 32-342 IN COMPLEXES WITH MOLYBDATE; TUNGSTATE; WTPB AND WTPC, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000782 Genomic DNA. Translation: AAB91135.1.

PIR

F69261.

RefSeq

NP_068935.1. NC_000917.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ONK	X-ray	3.10	E/J	32-342	[»]
2ONR	X-ray	1.60	A	32-342	[»]
2ONS	X-ray	1.55	A	32-342	[»]
3CIJ	X-ray	1.07	A/B	32-323	[»]

ProteinModelPortal

O30142.

SMR

O30142. Positions 32-342.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1483306.

GenomeReviews

Gene locus AF_0094 in contig AE000782_GR.

KEGG

afu:AF0094.

NMPDR

fig|224325.1.peg.94.

TIGR

AF_0094.

Phylogenomic databases

HOGENOM

HBG457285.

OMA

CGYRTQM.

ProtClustDB

PRK04168.

Enzyme and pathway databases

BioCyc

AFUL224325:AF_0094-MONOMER.

Family and domain databases

InterPro

IPR022498. ABC_trnspt_W-bd_WtpA.
IPR006059. Solute-bd_1_bac.
[Graphical view]

KO

K15495.

Pfam

PF01547. SBP_bac_1. 1 hit.
[Graphical view]

TIGRFAMs

TIGR03730. Tungstate_WtpA. 1 hit.

ProtoNet

Search...

Entry information

Entry name

WTPA_ARCFU

Accession

Primary (citable) accession number: O30142

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program