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Protein

NAD-dependent protein deacylase 2

Gene

cobB2

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NAD-dependent protein deacetylase which modulates the activities of several proteins which are inactive in their acetylated form. Deacetylates the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription.UniRule annotation1 Publication

Catalytic activityi

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.UniRule annotation

Cofactori

Zn2+UniRule annotation3 PublicationsNote: Binds 1 zinc ion per subunit.UniRule annotation3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei118Proton acceptor1
Metal bindingi126ZincUniRule annotation2 Publications1
Metal bindingi129ZincUniRule annotation2 Publications1
Metal bindingi150ZincUniRule annotation2 Publications1
Metal bindingi153ZincUniRule annotation2 Publications1
Binding sitei235NAD; via amide nitrogenUniRule annotation2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi23 – 42NADUniRule annotation2 PublicationsAdd BLAST20
Nucleotide bindingi100 – 103NADUniRule annotation2 Publications4
Nucleotide bindingi191 – 193NADUniRule annotation2 Publications3
Nucleotide bindingi217 – 219NADUniRule annotation2 Publications3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, NAD, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent protein deacylase 2UniRule annotation (EC:3.5.1.-UniRule annotation)
Alternative name(s):
Regulatory protein SIR2 homolog 2UniRule annotation
SIR2-Af2
Gene namesi
Name:cobB2UniRule annotation
Synonyms:Sir2Af2
Ordered Locus Names:AF_0112
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001103781 – 253NAD-dependent protein deacylase 2Add BLAST253

Interactioni

Protein-protein interaction databases

STRINGi224325.AF0112.

Structurei

Secondary structure

1253
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 14Combined sources13
Beta strandi16 – 22Combined sources7
Helixi24 – 26Combined sources3
Helixi41 – 44Combined sources4
Helixi48 – 51Combined sources4
Helixi53 – 58Combined sources6
Helixi60 – 73Combined sources14
Helixi80 – 90Combined sources11
Beta strandi94 – 99Combined sources6
Helixi105 – 108Combined sources4
Beta strandi113 – 116Combined sources4
Beta strandi119 – 126Combined sources8
Turni127 – 129Combined sources3
Beta strandi132 – 134Combined sources3
Helixi135 – 137Combined sources3
Helixi139 – 143Combined sources5
Turni151 – 153Combined sources3
Beta strandi158 – 162Combined sources5
Helixi171 – 182Combined sources12
Beta strandi185 – 191Combined sources7
Turni197 – 200Combined sources4
Helixi201 – 208Combined sources8
Beta strandi212 – 219Combined sources8
Helixi224 – 226Combined sources3
Beta strandi228 – 233Combined sources6
Helixi235 – 249Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MA3X-ray2.00A1-253[»]
1S7GX-ray2.30A/B/C/D/E1-253[»]
1YC2X-ray2.40A/B/C/D/E1-253[»]
4TWJX-ray1.65A1-253[»]
ProteinModelPortaliO30124.
SMRiO30124.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO30124.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 251Deacetylase sirtuin-typeUniRule annotationAdd BLAST246

Sequence similaritiesi

Belongs to the sirtuin family. Class III subfamily.UniRule annotation
Contains 1 deacetylase sirtuin-type domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04248. Archaea.
COG0846. LUCA.
KOiK12410.
OMAiHARIIEM.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
HAMAPiMF_01121. Sirtuin_ClassIII. 1 hit.
MF_01968. Sirtuin_ClassU. 1 hit.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR027546. Sirtuin_class_III.
IPR028628. Sirtuin_class_U.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 1 hit.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O30124-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDEIRKAAE ILAKSKHAVV FTGAGISAES GIPTFRGEDG LWRKYDPEEV
60 70 80 90 100
ASISGFKRNP RAFWEFSMEM KDKLFAEPNP AHYAIAELER MGIVKAVITQ
110 120 130 140 150
NIDMLHQRAG SRRVLELHGS MDKLDCLDCH ETYDWSEFVE DFNKGEIPRC
160 170 180 190 200
RKCGSYYVKP RVVLFGEPLP QRTLFEAIEE AKHCDAFMVV GSSLVVYPAA
210 220 230 240 250
ELPYIAKKAG AKMIIVNAEP TMADPIFDVK IIGKAGEVLP KIVEEVKRLR

SEK
Length:253
Mass (Da):28,495
Last modified:January 1, 1998 - v1
Checksum:i71A243C9012CBBFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB91115.1.
PIRiH69263.
RefSeqiWP_010877626.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB91115; AAB91115; AF_0112.
GeneIDi24793666.
KEGGiafu:AF_0112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB91115.1.
PIRiH69263.
RefSeqiWP_010877626.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MA3X-ray2.00A1-253[»]
1S7GX-ray2.30A/B/C/D/E1-253[»]
1YC2X-ray2.40A/B/C/D/E1-253[»]
4TWJX-ray1.65A1-253[»]
ProteinModelPortaliO30124.
SMRiO30124.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF0112.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB91115; AAB91115; AF_0112.
GeneIDi24793666.
KEGGiafu:AF_0112.

Phylogenomic databases

eggNOGiarCOG04248. Archaea.
COG0846. LUCA.
KOiK12410.
OMAiHARIIEM.

Miscellaneous databases

EvolutionaryTraceiO30124.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
HAMAPiMF_01121. Sirtuin_ClassIII. 1 hit.
MF_01968. Sirtuin_ClassU. 1 hit.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR027546. Sirtuin_class_III.
IPR028628. Sirtuin_class_U.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 1 hit.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNPD2_ARCFU
AccessioniPrimary (citable) accession number: O30124
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The two SIR2 homologs in this organism, Af1 and Af2, display different substrate specificities in vitro. Af2 can deacetylate histones and peptides corresponding to the C-terminal part of p53 in a NAD-dependent manner (PubMed:12408821).1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.