O30020 (LEU1_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 2-isopropylmalate synthase
EC=2.3.3.13

Alternative name(s):
Alpha-IPM synthase
Alpha-isopropylmalate synthase

Gene names

Name:	leuA
Ordered Locus Names:	AF_0219

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) By similarity.
Catalytic activity	Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.
Sequence similarities	Belongs to the alpha-IPM synthase/homocitrate synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-isopropylmalate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Probable 2-isopropylmalate synthase		PRO_0000140407

Sequences

	Sequence		Length	Mass (Da)
	O30020 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 67F8FD363D0A20FD	FASTA	503	55,212
	10 20 30 40 50 60 MSAVMTTIRV FDTTLRDGEQ MPGVSLPLEY KIRIAKQLDK LGVDVIEAGF PSATKGEFES 70 80 90 100 110 120 VKEISSLSLN AEICGLARIV KEDIDAAINA NVDMVHIFVP TSRIQIEHTV KMSKEEIIEK 130 140 150 160 170 180 SVECVEYIKS HGVKCMFSAM DATRTEVEYL KQIFKAVENA KVDIVNVPDT VGVATPFKFY 190 200 210 220 230 240 ELIKQLREHL KVPIDVHCHN DFGLAVANTY AAVMAGANEV QVTVNGIGER AGNADLAQVV 250 260 270 280 290 300 MILHAIEGIK TNIKTEHLFE TSKLVERLTG VKMPPNHPIV GENAFSHESG IHAHGVLKEY 310 320 330 340 350 360 STFEPGVVTP EMVGHKRRIV IGKHAGRYQI KKILEEAGYH LDDEKLNQIF EKVKEMGDKG 370 380 390 400 410 420 KRVTDLDLFA IAEVVIGELK REEKAILVDE VTVLTGNKIT PTAVLNAEVF GHRKVTSAIG 430 440 450 460 470 480 VGPVDASLKA VTSLVGESIR ITEFRMDAIT GGSDALAEVY VTVEDDEGHS FTSRGAAQDI 490 500 VMASIDAVIN AVNYLLRMKR RKS

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB91014.1.
PIR	C69277.
RefSeq	NP_069057.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O30020.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1483430.
GenomeReviews	Gene locus AF_0219 in contig AE000782_GR.
KEGG	afu:AF0219.
NMPDR	fig|224325.1.peg.216.
TIGR	AF_0219.
Phylogenomic databases
HOGENOM	HBG701974.
OMA	NNPFTYE.
PhylomeDB	O30020.
ProtClustDB	PRK00915.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_0219-MONOMER.
Family and domain databases
InterPro	IPR013709. 2-isopropylmalate_synth_dimer. IPR002034. AIPM/Hcit_synth_CS. IPR013785. Aldolase_TIM. IPR011830. LEU1_arch. IPR000891. PYR_CT. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01649.
Pfam	PF00682. HMGL-like. 1 hit. PF08502. LeuA_dimer. 1 hit. [Graphical view]
SMART	SM00917. LeuA_dimer. 1 hit. [Graphical view]
SUPFAM	SSF110921. 2-isopropylmalate_synth_dimer. 1 hit.
TIGRFAMs	TIGR02090. LEU1_arch. 1 hit.
PROSITE	PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit. PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit. PS50991. PYR_CT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU1_ARCFU

Accession

Primary (citable) accession number: O30020

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program