Skip Header

Contribute Send feedback
Read comments (?) or add your own

O30019 (PYCA_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate carboxylase subunit A
EC=6.4.1.1
Alternative name(s):
Pyruvic carboxylase A
Gene names
Name:pycA
Ordered Locus Names:AF_0220
OrganismArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifier224325 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length506 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.

Catalytic activity

ATP + pyruvate + HCO3- = ADP + phosphate + oxaloacetate.

Cofactor

Magnesium or manganese or cobalt By similarity.

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Subunit structure

Heterooctamer of four A and four B subunits By similarity.

Sequence similarities

Contains 1 ATP-grasp domain.

Contains 1 biotin carboxylation domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 506506Pyruvate carboxylase subunit A
PRO_0000146827

Regions

Domain1 – 443443Biotin carboxylation

Sites

Active site2901 Potential
Binding site1161ATP By similarity
Binding site1991ATP By similarity
Binding site2341ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
O30019 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 39B765F319235AD1

FASTA50657,438
        10         20         30         40         50         60 
MFSKILVANR GEIAVRVMRA CRELGIKTVG VYSSADKRAF HRVYADECYY IGKADPRDSY 

        70         80         90        100        110        120 
LNIDRIIEVA KKSGAEAIHP GYGFLAENAE FAERCEEEGI VFIGPSPEVI RIAGSKVRSR 

       130        140        150        160        170        180 
ESMQRAGVPV IPGSPKIDTV DEAKEWAEKI GYPVAVKASG GGGGIGIVVV NSQEELEEAF 

       190        200        210        220        230        240 
RKSKKLGESY FKDSTVYLEK YLARPRHIEV QILADQHGNV IHLGERECSI QRRHQKLIEE 

       250        260        270        280        290        300 
APSPALNEEM REELGKLAVK GAREIGYTNA GTFEFLYENG NFYFLEINSR LQVEHTITEV 

       310        320        330        340        350        360 
VTGIDIVKYQ IRIAYGEELR HGQEDVAIRG HAIECRINAE DPVNFYPRSG RILHYRSPGG 

       370        380        390        400        410        420 
IGIRVDSGIH MGYRIPEEYD SMISKLIAYG ETREEAIARM KRALYEYIIE GVETNIPFHF 

       430        440        450        460        470        480 
AVLNDEEFVR GNIHTKFVEE RNIAEKVKEY LRIFRPIKAR LDEIFMESEF TWEEISAIVT 

       490        500 
AIDAYEQELE RGIEERIWQA IFSLGA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000782 Genomic DNA. Translation: AAB91012.1.
PIRD69277.
RefSeqNP_069058.1. NC_000917.1.

3D structure databases

ProteinModelPortalO30019.
SMRO30019. Positions 1-441.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1483431.
GenomeReviewsGene locus AF_0220 in contig AE000782_GR.
KEGGafu:AF0220.
NMPDRfig|224325.1.peg.217.
TIGRAF_0220.

Phylogenomic databases

HOGENOMHBG535236.
OMASTHYVED.
PhylomeDBO30019.
ProtClustDBPRK08654.

Enzyme and pathway databases

BioCycAFUL224325:AF_0220-MONOMER.

Family and domain databases

InterProIPR004549. Acetyl_CoA_COase_biotin_COase.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR005479. CarbamoylP_synth_lsu_ATP-bd.
IPR005481. CarbamoylP_synth_lsu_N.
IPR013817. Pre-ATP_grasp.
IPR016185. PreATP-grasp-like.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
Gene3DG3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit.
G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KOK01959.
PfamPF02785. Biotin_carb_C. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMsTIGR00514. AccC. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYCA_ARCFU
AccessionPrimary (citable) accession number: O30019
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents