O30011 (AROD_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	AF_0228

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 196

196

3-dehydroquinate dehydratase HAMAP MF_00214

PRO_0000138826

Sites

Active site

98

1

Proton acceptor By similarity

Active site

122

1

Schiff-base intermediate with substrate By similarity

Secondary structure

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196

Helix Strand Turn

Details...

Beta strand

2 – 7

6

Helix

10 – 15

6

Turn

16 – 18

3

Beta strand

20 – 25

6

Turn

26 – 28

3

Beta strand

38 – 43

6

Helix

47 – 49

3

Beta strand

51 – 53

3

Helix

57 – 71

15

Beta strand

74 – 79

6

Helix

84 – 86

3

Beta strand

90 – 101

12

Helix

106 – 114

9

Beta strand

118 – 126

9

Helix

130 – 142

13

Beta strand

144 – 152

9

Helix

153 – 156

4

Helix

157 – 164

8

Beta strand

168 – 176

9

Helix

185 – 195

11

Sequences

	Sequence		Length	Mass (Da)
	O30011 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 9BF77DEB9B984B8B	FASTA	196	22,207
	10 20 30 40 50 60 MKLVATLSSP EELELAEKAD VVELRIDLFD FSGARVDKEK ILTCRRVSDG GKFEGDERER 70 80 90 100 110 120 IEKMKRAFDS LNPDYVDLES DLPDSAFDFN CRIIESYHNF IRTPDYSELK GIVEGRRGDL 130 140 150 160 170 180 VKIATMGKSK RDVETIVRIL TNYDDVVAFL MGERFSFTRV LAAYLGSPFI YCYVGSPKAP 190 GQISLDDARE IISRLG

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

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Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000782 Genomic DNA. Translation: AAB91004.1.

PIR

D69278.

RefSeq

NP_069066.1. NC_000917.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2OX1	X-ray	2.33	A/B/C/D	1-196	[»]

ProteinModelPortal

O30011.

SMR

O30011. Positions 1-196.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1483439.

GenomeReviews

Gene locus AF_0228 in contig AE000782_GR.

KEGG

afu:AF0228.

NMPDR

fig|224325.1.peg.225.

TIGR

AF_0228.

Phylogenomic databases

HOGENOM

HBG732926.

OMA

IESYHNF.

ProtClustDB

CLSK2746348.

Enzyme and pathway databases

BioCyc

AFUL224325:AF_0228-MONOMER.

Family and domain databases

HAMAP

MF_00214. AroD.
[Tree]

InterPro

IPR013785. Aldolase_TIM.
IPR001381. DHquinase_I.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

KO

K03785.

Pfam

PF01487. DHquinase_I. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01093. AroD. 1 hit.

PROSITE

PS01028. DEHYDROQUINASE_I. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AROD_ARCFU

Accession

Primary (citable) accession number: O30011

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program