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Protein

3-dehydroquinate dehydratase

Gene

aroD

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.UniRule annotation

Catalytic activityi

3-dehydroquinate = 3-dehydroshikimate + H2O.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. no protein annotated in this organism
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 4513-dehydroquinateUniRule annotation
Active sitei98 – 981Proton donor/acceptorUniRule annotation
Active sitei122 – 1221Schiff-base intermediate with substrateUniRule annotation
Binding sitei159 – 15913-dehydroquinateUniRule annotation
Binding sitei182 – 18213-dehydroquinateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-232-MONOMER.
BRENDAi4.2.1.10. 414.
UniPathwayiUPA00053; UER00086.

Names & Taxonomyi

Protein namesi
Recommended name:
3-dehydroquinate dehydrataseUniRule annotation (EC:4.2.1.10UniRule annotation)
Short name:
3-dehydroquinaseUniRule annotation
Alternative name(s):
Type I DHQaseUniRule annotation
Type I dehydroquinaseUniRule annotation
Short name:
DHQ1UniRule annotation
Gene namesi
Name:aroDUniRule annotation
Ordered Locus Names:AF_0228
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1961963-dehydroquinate dehydratasePRO_0000138826Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi224325.AF0228.

Structurei

Secondary structure

1
196
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Helixi10 – 156Combined sources
Turni16 – 183Combined sources
Beta strandi20 – 256Combined sources
Turni26 – 283Combined sources
Beta strandi38 – 436Combined sources
Helixi47 – 493Combined sources
Beta strandi51 – 533Combined sources
Helixi57 – 7115Combined sources
Beta strandi74 – 796Combined sources
Helixi84 – 863Combined sources
Beta strandi90 – 10112Combined sources
Helixi106 – 1149Combined sources
Beta strandi118 – 1269Combined sources
Helixi130 – 14213Combined sources
Beta strandi144 – 1529Combined sources
Helixi153 – 1564Combined sources
Helixi157 – 1648Combined sources
Beta strandi168 – 1769Combined sources
Helixi185 – 19511Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OX1X-ray2.33A/B/C/D1-196[»]
ProteinModelPortaliO30011.
SMRiO30011. Positions 1-196.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO30011.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 2533-dehydroquinate bindingUniRule annotation

Sequence similaritiesi

Belongs to the type-I 3-dehydroquinase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG02097. Archaea.
COG0710. LUCA.
KOiK03785.
OMAiIESYHNF.

Family and domain databases

CDDicd00502. DHQase_I. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00214. AroD. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001381. DHquinase_I.
[Graphical view]
PfamiPF01487. DHquinase_I. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01093. aroD. 1 hit.

Sequencei

Sequence statusi: Complete.

O30011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLVATLSSP EELELAEKAD VVELRIDLFD FSGARVDKEK ILTCRRVSDG
60 70 80 90 100
GKFEGDERER IEKMKRAFDS LNPDYVDLES DLPDSAFDFN CRIIESYHNF
110 120 130 140 150
IRTPDYSELK GIVEGRRGDL VKIATMGKSK RDVETIVRIL TNYDDVVAFL
160 170 180 190
MGERFSFTRV LAAYLGSPFI YCYVGSPKAP GQISLDDARE IISRLG
Length:196
Mass (Da):22,207
Last modified:January 1, 1998 - v1
Checksum:i9BF77DEB9B984B8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB91004.1.
PIRiD69278.
RefSeqiWP_010877739.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB91004; AAB91004; AF_0228.
GeneIDi24793762.
KEGGiafu:AF_0228.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB91004.1.
PIRiD69278.
RefSeqiWP_010877739.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OX1X-ray2.33A/B/C/D1-196[»]
ProteinModelPortaliO30011.
SMRiO30011. Positions 1-196.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF0228.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB91004; AAB91004; AF_0228.
GeneIDi24793762.
KEGGiafu:AF_0228.

Phylogenomic databases

eggNOGiarCOG02097. Archaea.
COG0710. LUCA.
KOiK03785.
OMAiIESYHNF.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00086.
BioCyciAFUL224325:GJBC-232-MONOMER.
BRENDAi4.2.1.10. 414.

Miscellaneous databases

EvolutionaryTraceiO30011.

Family and domain databases

CDDicd00502. DHQase_I. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00214. AroD. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001381. DHquinase_I.
[Graphical view]
PfamiPF01487. DHquinase_I. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01093. aroD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROD_ARCFU
AccessioniPrimary (citable) accession number: O30011
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.