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Protein

Archaeal Lon protease

Gene

AF_0364

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei5091
Active sitei509PROSITE-ProRule annotation1
Active sitei5521
Active sitei552PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi54 – 61ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.4.21.53. 414.

Protein family/group databases

MEROPSiS16.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Archaeal Lon protease (EC:3.4.21.-)
Alternative name(s):
ATP-dependent protease La homolog
Gene namesi
Ordered Locus Names:AF_0364
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 117CytoplasmicSequence analysisAdd BLAST117
Transmembranei118 – 136HelicalSequence analysisAdd BLAST19
Topological domaini137 – 141ExtracellularSequence analysis5
Transmembranei142 – 160HelicalSequence analysisAdd BLAST19
Topological domaini161 – 621CytoplasmicSequence analysisAdd BLAST461

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi506E → A: Slightly decreases proteolytic activity. 1
Mutagenesisi508D → A: No effect. 1
Mutagenesisi509S → A: Completely abolishes proteolytic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000761511 – 621Archaeal Lon proteaseAdd BLAST621

Interactioni

Subunit structurei

Homohexamer. Organized in a ring with a central cavity (By similarity).By similarity

Protein-protein interaction databases

STRINGi224325.AF0364.

Structurei

Secondary structure

1621
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi422 – 425Combined sources4
Beta strandi428 – 435Combined sources8
Turni436 – 438Combined sources3
Beta strandi439 – 451Combined sources13
Helixi467 – 485Combined sources19
Helixi489 – 491Combined sources3
Beta strandi492 – 500Combined sources9
Beta strandi503 – 506Combined sources4
Helixi512 – 523Combined sources12
Beta strandi531 – 533Combined sources3
Beta strandi541 – 544Combined sources4
Helixi549 – 558Combined sources10
Beta strandi562 – 567Combined sources6
Helixi568 – 573Combined sources6
Turni578 – 582Combined sources5
Beta strandi583 – 591Combined sources9
Helixi592 – 599Combined sources8
Helixi604 – 620Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z0BX-ray1.55A415-621[»]
1Z0CX-ray1.55A415-621[»]
1Z0EX-ray2.05A/B/C/D/E/F417-621[»]
1Z0GX-ray2.27A/B/C/D/E/F417-621[»]
1Z0TX-ray3.00A/B/C/D/E/F417-621[»]
1Z0VX-ray3.00A/B/C/D/E/F417-621[»]
1Z0WX-ray1.20A415-621[»]
ProteinModelPortaliO29883.
SMRiO29883.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO29883.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini423 – 602Lon proteolyticPROSITE-ProRule annotationAdd BLAST180

Sequence similaritiesi

Contains 1 Lon proteolytic domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiarCOG02160. Archaea.
COG1067. LUCA.
KOiK04076.
OMAiDVHIQFV.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR004663. Lon_arc.
IPR027065. Lon_Prtase.
IPR000523. Mg_chelatse_chII_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR002078. Sigma_54_int.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 2 hits.
PfamiPF05362. Lon_C. 1 hit.
PF01078. Mg_chelatase. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF54211. SSF54211. 1 hit.
TIGRFAMsiTIGR00764. lon_rel. 1 hit.
PROSITEiPS51786. LON_PROTEOLYTIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O29883-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEEVREILG GLDFESTKDV AVPERLIDQV IGQDHAVEAI KKAAVQKRHV
60 70 80 90 100
MLIGSPGTGK SMLAKAMAEL LPKEELEDIL VYPNPQDPNQ PKIRLVPAGK
110 120 130 140 150
GREIVEAYKE EAMKKAQARN FLIFTLVFLV IGYTVLTNPG NLIWGIIAAV
160 170 180 190 200
LILMMSRYFI PREDRNVPKL LVDNSDKVTA PFEDATGAHA GALFGDVRHD
210 220 230 240 250
PFQSGGLETP AHERVEAGAI HRAHKGVLYI DEINTLTIES QQKLLTALQD
260 270 280 290 300
KKFPITGQSE RSSGAMVRTE PVPCDFILVA AGNLDALMGM HPALRSRIEG
310 320 330 340 350
YGYEVYMNDT MPDTPENRQK LVRFVAQEVV KDGKIPHFDK YAVAEIIKEA
360 370 380 390 400
RRRAGRRNHL TLRLRELGGL VRTAGDIAKS EGSDIVRLEH VLKAKKIAKT
410 420 430 440 450
IEEQLADKYI ERRKDYKLFI TEGYEVGRVN GLAVIGESAG IVLPIIAEVT
460 470 480 490 500
PSMSKSEGRV IATGRLQEIA REAVMNVSAI IKKYTGRDIS NMDVHIQFVG
510 520 530 540 550
TYEGVEGDSA SISIATAVIS AIEGIPVDQS VAMTGSLSVK GEVLPVGGVT
560 570 580 590 600
QKIEAAIQAG LKKVIIPKDN IDDVLLDAEH EGKIEVIPVS RINEVLEHVL
610 620
EDGKKKNRLM SKFKELELAA V
Length:621
Mass (Da):68,208
Last modified:January 1, 1998 - v1
Checksum:iCC18C401C6799B3A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90868.1.
PIRiD69295.
RefSeqiWP_010877871.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90868; AAB90868; AF_0364.
GeneIDi1483579.
KEGGiafu:AF_0364.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90868.1.
PIRiD69295.
RefSeqiWP_010877871.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z0BX-ray1.55A415-621[»]
1Z0CX-ray1.55A415-621[»]
1Z0EX-ray2.05A/B/C/D/E/F417-621[»]
1Z0GX-ray2.27A/B/C/D/E/F417-621[»]
1Z0TX-ray3.00A/B/C/D/E/F417-621[»]
1Z0VX-ray3.00A/B/C/D/E/F417-621[»]
1Z0WX-ray1.20A415-621[»]
ProteinModelPortaliO29883.
SMRiO29883.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF0364.

Protein family/group databases

MEROPSiS16.005.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90868; AAB90868; AF_0364.
GeneIDi1483579.
KEGGiafu:AF_0364.

Phylogenomic databases

eggNOGiarCOG02160. Archaea.
COG1067. LUCA.
KOiK04076.
OMAiDVHIQFV.

Enzyme and pathway databases

BRENDAi3.4.21.53. 414.

Miscellaneous databases

EvolutionaryTraceiO29883.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR004663. Lon_arc.
IPR027065. Lon_Prtase.
IPR000523. Mg_chelatse_chII_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR002078. Sigma_54_int.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 2 hits.
PfamiPF05362. Lon_C. 1 hit.
PF01078. Mg_chelatase. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF54211. SSF54211. 1 hit.
TIGRFAMsiTIGR00764. lon_rel. 1 hit.
PROSITEiPS51786. LON_PROTEOLYTIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLONB_ARCFU
AccessioniPrimary (citable) accession number: O29883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.