O29836 (SYC_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	AF_0411

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) [Reference proteome] [HAMAP]

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus › Archaeoglobus fulgidus

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP-Rule MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Cysteine--tRNA ligase HAMAP-Rule MF_00041		PRO_0000159533
Regions
	Motif	30 – 40	11	"HIGH" region HAMAP-Rule MF_00041
	Motif	268 – 272	5	"KMSKS" region HAMAP-Rule MF_00041
Sites
	Metal binding	28	1	Zinc By similarity
	Metal binding	211	1	Zinc By similarity
	Metal binding	236	1	Zinc By similarity
	Metal binding	240	1	Zinc By similarity
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O29836 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 4494E21E8E88A961	FASTA	467	54,790
	10 20 30 40 50 60 MEVYNTLSRK IEKLEDIVDG KRVKMYVCGI TAYDYSHIGH ARSAVFFDVF RRYLEYLGYE 70 80 90 100 110 120 VVYVQNFTDV DDKIINRAVK EGKTQKEVAE KFIEEYLKDM EALNVKKPTY QPKVTEHIPD 130 140 150 160 170 180 IIEFIQNLIE KGYAYVIDGD VYFHVPAFEH YGELSKQSLE ELNRHRIEPD ERKRDVKDFA 190 200 210 220 230 240 LWKSAKEADL KAQAVFDSPW GRGRPGWHIE CSVMSAKYLG VPFDIHGGGK DLIFPHHENE 250 260 270 280 290 300 RAQSFARFGV EPVKIWVHND FIRIKGEKMS KSLGNIVRIR DVLQRYEGEV LRYFLLTAHY 310 320 330 340 350 360 RSPLDYTEEA LERAKRAYEY LRSALINLDM EIAYLKTFGD RKEGNQVDVE DYIRRFEEAM 370 380 390 400 410 420 NRDLHTPDAI AVLHEFAGLI NKSLYELSLN QAEELYEAFK RLCGVLGLFE KMERVPALSR 430 440 450 460 EDAEKVVERE RARKERNFEL ADAIRDEFAK RGIRLIDTPK GTRWRVE

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB90823.1.
PIR	C69301.
RefSeq	NP_069247.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O29836.
ModBase	Search...
Protein-protein interaction databases
STRING	224325.AF0411.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB90823; AAB90823; AF_0411.
GeneID	1483627.
KEGG	afu:AF0411.
Phylogenomic databases
eggNOG	COG0215.
KO	K01883.
OMA	DFDALNM.
ProtClustDB	CLSK2746427.
Enzyme and pathway databases
BioCyc	AFUL224325:GJBC-422-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00041. Cys_tRNA_synth.
InterPro	IPR015803. Cys-tRNA-ligase. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR10890. PTHR10890. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ARCFU

Accession

Primary (citable) accession number: O29836

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program