Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi28 – 281ZincBy similarity
Metal bindingi211 – 2111ZincBy similarity
Metal bindingi236 – 2361ZincBy similarity
Metal bindingi240 – 2401ZincBy similarity
Binding sitei271 – 2711ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-422-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligase (EC:6.1.1.16)
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name:
CysRS
Gene namesi
Name:cysS
Ordered Locus Names:AF_0411
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 467467Cysteine--tRNA ligasePRO_0000159533Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224325.AF0411.

Structurei

3D structure databases

ProteinModelPortaliO29836.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi30 – 4011"HIGH" regionAdd
BLAST
Motifi268 – 2725"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00486. Archaea.
COG0215. LUCA.
KOiK01883.
OMAiHENEACQ.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequencei

Sequence statusi: Complete.

O29836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVYNTLSRK IEKLEDIVDG KRVKMYVCGI TAYDYSHIGH ARSAVFFDVF
60 70 80 90 100
RRYLEYLGYE VVYVQNFTDV DDKIINRAVK EGKTQKEVAE KFIEEYLKDM
110 120 130 140 150
EALNVKKPTY QPKVTEHIPD IIEFIQNLIE KGYAYVIDGD VYFHVPAFEH
160 170 180 190 200
YGELSKQSLE ELNRHRIEPD ERKRDVKDFA LWKSAKEADL KAQAVFDSPW
210 220 230 240 250
GRGRPGWHIE CSVMSAKYLG VPFDIHGGGK DLIFPHHENE RAQSFARFGV
260 270 280 290 300
EPVKIWVHND FIRIKGEKMS KSLGNIVRIR DVLQRYEGEV LRYFLLTAHY
310 320 330 340 350
RSPLDYTEEA LERAKRAYEY LRSALINLDM EIAYLKTFGD RKEGNQVDVE
360 370 380 390 400
DYIRRFEEAM NRDLHTPDAI AVLHEFAGLI NKSLYELSLN QAEELYEAFK
410 420 430 440 450
RLCGVLGLFE KMERVPALSR EDAEKVVERE RARKERNFEL ADAIRDEFAK
460
RGIRLIDTPK GTRWRVE
Length:467
Mass (Da):54,790
Last modified:January 1, 1998 - v1
Checksum:i4494E21E8E88A961
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90823.1.
PIRiC69301.
RefSeqiWP_010877918.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90823; AAB90823; AF_0411.
GeneIDi1483627.
KEGGiafu:AF_0411.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90823.1.
PIRiC69301.
RefSeqiWP_010877918.1. NC_000917.1.

3D structure databases

ProteinModelPortaliO29836.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF0411.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90823; AAB90823; AF_0411.
GeneIDi1483627.
KEGGiafu:AF_0411.

Phylogenomic databases

eggNOGiarCOG00486. Archaea.
COG0215. LUCA.
KOiK01883.
OMAiHENEACQ.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-422-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Entry informationi

Entry nameiSYC_ARCFU
AccessioniPrimary (citable) accession number: O29836
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.