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Protein

Proteasome subunit alpha

Gene

psmA

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.UniRule annotation

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.UniRule annotation

Enzyme regulationi

The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Protein family/group databases

MEROPSiT01.973.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alphaUniRule annotation (EC:3.4.25.1UniRule annotation)
Alternative name(s):
20S proteasome alpha subunitUniRule annotation
Proteasome core protein PsmAUniRule annotation
Gene namesi
Name:psmAUniRule annotation
Ordered Locus Names:AF_0490
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001241671 – 246Proteasome subunit alphaAdd BLAST246

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has probably a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is likely capped at one or both ends by the proteasome regulatory ATPase, PAN.1 Publication

Protein-protein interaction databases

IntActiO29760. 1 interactor.
STRINGi224325.AF0490.

Structurei

Secondary structure

1246
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 10Combined sources3
Helixi22 – 32Combined sources11
Beta strandi37 – 42Combined sources6
Beta strandi45 – 51Combined sources7
Helixi61 – 63Combined sources3
Beta strandi66 – 69Combined sources4
Beta strandi71 – 80Combined sources10
Helixi82 – 102Combined sources21
Beta strandi103 – 105Combined sources3
Helixi109 – 122Combined sources14
Turni123 – 125Combined sources3
Beta strandi127 – 129Combined sources3
Beta strandi134 – 150Combined sources17
Beta strandi156 – 165Combined sources10
Helixi168 – 178Combined sources11
Helixi185 – 200Combined sources16
Helixi206 – 208Combined sources3
Beta strandi209 – 215Combined sources7
Turni216 – 218Combined sources3
Helixi226 – 243Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J2PX-ray2.60A/B/C/D/E/F/G1-246[»]
1J2QX-ray2.83A/B/C/D/E/F/G10-246[»]
ProteinModelPortaliO29760.
SMRiO29760.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO29760.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00971. Archaea.
COG0638. LUCA.
KOiK03432.
OMAiEVAIMAP.

Family and domain databases

CDDicd03756. proteasome_alpha_archeal. 1 hit.
Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00289_A. Proteasome_A_A. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR019982. Proteasome_asu_arc.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03633. arc_protsome_A. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O29760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLPQMGYDR AITVFSPDGR LFQVEYAREA VKRGATAIGI KCKEGVILIA
60 70 80 90 100
DKRVGSKLLE ADTIEKIYKI DEHICAATSG LVADARVLID RARIEAQINR
110 120 130 140 150
LTYDEPITVK ELAKKICDFK QQYTQYGGVR PFGVSLLIAG VDEVPKLYET
160 170 180 190 200
DPSGALLEYK ATAIGMGRNA VTEFFEKEYR DDLSFDDAMV LGLVAMGLSI
210 220 230 240
ESELVPENIE VGYVKVDDRT FKEVSPEELK PYVERANERI RELLKK
Length:246
Mass (Da):27,599
Last modified:January 1, 1998 - v1
Checksum:i9F24A7F120ADBE5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90747.1.
PIRiB69311.
RefSeqiWP_010877997.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90747; AAB90747; AF_0490.
GeneIDi24794030.
KEGGiafu:AF_0490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90747.1.
PIRiB69311.
RefSeqiWP_010877997.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J2PX-ray2.60A/B/C/D/E/F/G1-246[»]
1J2QX-ray2.83A/B/C/D/E/F/G10-246[»]
ProteinModelPortaliO29760.
SMRiO29760.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO29760. 1 interactor.
STRINGi224325.AF0490.

Protein family/group databases

MEROPSiT01.973.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90747; AAB90747; AF_0490.
GeneIDi24794030.
KEGGiafu:AF_0490.

Phylogenomic databases

eggNOGiarCOG00971. Archaea.
COG0638. LUCA.
KOiK03432.
OMAiEVAIMAP.

Miscellaneous databases

EvolutionaryTraceiO29760.

Family and domain databases

CDDicd03756. proteasome_alpha_archeal. 1 hit.
Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00289_A. Proteasome_A_A. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR019982. Proteasome_asu_arc.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03633. arc_protsome_A. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSA_ARCFU
AccessioniPrimary (citable) accession number: O29760
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.