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O29760

- PSA_ARCFU

UniProt

O29760 - PSA_ARCFU

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Protein

Proteasome subunit alpha

Gene

psmA

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.UniRule annotation

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.UniRule annotation

Enzyme regulationi

The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-503-MONOMER.

Protein family/group databases

MEROPSiT01.973.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alphaUniRule annotation (EC:3.4.25.1UniRule annotation)
Alternative name(s):
20S proteasome alpha subunitUniRule annotation
Proteasome core protein PsmAUniRule annotation
Gene namesi
Name:psmAUniRule annotation
Ordered Locus Names:AF_0490
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
ProteomesiUP000002199: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. proteasome core complex, alpha-subunit complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 246246Proteasome subunit alphaPRO_0000124167Add
BLAST

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has probably a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is likely capped at one or both ends by the proteasome regulatory ATPase, PAN.1 Publication

Protein-protein interaction databases

IntActiO29760. 1 interaction.
STRINGi224325.AF0490.

Structurei

Secondary structure

1
246
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 103Combined sources
Helixi22 – 3211Combined sources
Beta strandi37 – 426Combined sources
Beta strandi45 – 517Combined sources
Helixi61 – 633Combined sources
Beta strandi66 – 694Combined sources
Beta strandi71 – 8010Combined sources
Helixi82 – 10221Combined sources
Beta strandi103 – 1053Combined sources
Helixi109 – 12214Combined sources
Turni123 – 1253Combined sources
Beta strandi127 – 1293Combined sources
Beta strandi134 – 15017Combined sources
Beta strandi156 – 16510Combined sources
Helixi168 – 17811Combined sources
Helixi185 – 20016Combined sources
Helixi206 – 2083Combined sources
Beta strandi209 – 2157Combined sources
Turni216 – 2183Combined sources
Helixi226 – 24318Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J2PX-ray2.60A/B/C/D/E/F/G1-246[»]
1J2QX-ray2.83A/B/C/D/E/F/G10-246[»]
ProteinModelPortaliO29760.
SMRiO29760. Positions 10-246.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO29760.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0638.
KOiK03432.
OMAiFQVNYAR.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00289_A. Proteasome_A_A.
InterProiIPR029055. Ntn_hydrolases_N.
IPR019982. Proteasome_asu_arc.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03633. arc_protsome_A. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O29760-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHLPQMGYDR AITVFSPDGR LFQVEYAREA VKRGATAIGI KCKEGVILIA
60 70 80 90 100
DKRVGSKLLE ADTIEKIYKI DEHICAATSG LVADARVLID RARIEAQINR
110 120 130 140 150
LTYDEPITVK ELAKKICDFK QQYTQYGGVR PFGVSLLIAG VDEVPKLYET
160 170 180 190 200
DPSGALLEYK ATAIGMGRNA VTEFFEKEYR DDLSFDDAMV LGLVAMGLSI
210 220 230 240
ESELVPENIE VGYVKVDDRT FKEVSPEELK PYVERANERI RELLKK
Length:246
Mass (Da):27,599
Last modified:January 1, 1998 - v1
Checksum:i9F24A7F120ADBE5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90747.1.
PIRiB69311.
RefSeqiNP_069326.1. NC_000917.1.
WP_010877997.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90747; AAB90747; AF_0490.
GeneIDi1483707.
KEGGiafu:AF0490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90747.1 .
PIRi B69311.
RefSeqi NP_069326.1. NC_000917.1.
WP_010877997.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1J2P X-ray 2.60 A/B/C/D/E/F/G 1-246 [» ]
1J2Q X-ray 2.83 A/B/C/D/E/F/G 10-246 [» ]
ProteinModelPortali O29760.
SMRi O29760. Positions 10-246.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O29760. 1 interaction.
STRINGi 224325.AF0490.

Protein family/group databases

MEROPSi T01.973.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB90747 ; AAB90747 ; AF_0490 .
GeneIDi 1483707.
KEGGi afu:AF0490.

Phylogenomic databases

eggNOGi COG0638.
KOi K03432.
OMAi FQVNYAR.

Enzyme and pathway databases

BioCyci AFUL224325:GJBC-503-MONOMER.

Miscellaneous databases

EvolutionaryTracei O29760.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
HAMAPi MF_00289_A. Proteasome_A_A.
InterProi IPR029055. Ntn_hydrolases_N.
IPR019982. Proteasome_asu_arc.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view ]
SMARTi SM00948. Proteasome_A_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR03633. arc_protsome_A. 1 hit.
PROSITEi PS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
  2. "Investigations on the maturation and regulation of archaebacterial proteasomes."
    Groll M., Brandstetter H., Bartunik H., Bourenkow G., Huber R.
    J. Mol. Biol. 327:75-83(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF NATIVE PROTEIN AND IN COMPLEX WITH BETA SUBUNIT AND CALPAIN-INHIBITOR I, SUBUNIT.

Entry informationi

Entry nameiPSA_ARCFU
AccessioniPrimary (citable) accession number: O29760
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3