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O29760

- PSA_ARCFU

UniProt

O29760 - PSA_ARCFU

Protein

Proteasome subunit alpha

Gene

psmA

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.UniRule annotation

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.UniRule annotation

    Enzyme regulationi

    The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    BioCyciAFUL224325:GJBC-503-MONOMER.

    Protein family/group databases

    MEROPSiT01.973.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit alphaUniRule annotation (EC:3.4.25.1UniRule annotation)
    Alternative name(s):
    20S proteasome alpha subunitUniRule annotation
    Proteasome core protein PsmAUniRule annotation
    Gene namesi
    Name:psmAUniRule annotation
    Ordered Locus Names:AF_0490
    OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
    Taxonomic identifieri224325 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
    ProteomesiUP000002199: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. proteasome core complex, alpha-subunit complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 246246Proteasome subunit alphaPRO_0000124167Add
    BLAST

    Interactioni

    Subunit structurei

    The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has probably a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is likely capped at one or both ends by the proteasome regulatory ATPase, PAN.1 Publication

    Protein-protein interaction databases

    IntActiO29760. 1 interaction.
    STRINGi224325.AF0490.

    Structurei

    Secondary structure

    1
    246
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 103
    Helixi22 – 3211
    Beta strandi37 – 426
    Beta strandi45 – 517
    Helixi61 – 633
    Beta strandi66 – 694
    Beta strandi71 – 8010
    Helixi82 – 10221
    Beta strandi103 – 1053
    Helixi109 – 12214
    Turni123 – 1253
    Beta strandi127 – 1293
    Beta strandi134 – 15017
    Beta strandi156 – 16510
    Helixi168 – 17811
    Helixi185 – 20016
    Helixi206 – 2083
    Beta strandi209 – 2157
    Turni216 – 2183
    Helixi226 – 24318

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1J2PX-ray2.60A/B/C/D/E/F/G1-246[»]
    1J2QX-ray2.83A/B/C/D/E/F/G10-246[»]
    ProteinModelPortaliO29760.
    SMRiO29760. Positions 10-246.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO29760.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1A family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    KOiK03432.
    OMAiFQVNYAR.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    HAMAPiMF_00289_A. Proteasome_A_A.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR019982. Proteasome_asu_arc.
    IPR000426. Proteasome_asu_N.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view]
    SMARTiSM00948. Proteasome_A_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR03633. arc_protsome_A. 1 hit.
    PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O29760-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHLPQMGYDR AITVFSPDGR LFQVEYAREA VKRGATAIGI KCKEGVILIA    50
    DKRVGSKLLE ADTIEKIYKI DEHICAATSG LVADARVLID RARIEAQINR 100
    LTYDEPITVK ELAKKICDFK QQYTQYGGVR PFGVSLLIAG VDEVPKLYET 150
    DPSGALLEYK ATAIGMGRNA VTEFFEKEYR DDLSFDDAMV LGLVAMGLSI 200
    ESELVPENIE VGYVKVDDRT FKEVSPEELK PYVERANERI RELLKK 246
    Length:246
    Mass (Da):27,599
    Last modified:January 1, 1998 - v1
    Checksum:i9F24A7F120ADBE5E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000782 Genomic DNA. Translation: AAB90747.1.
    PIRiB69311.
    RefSeqiNP_069326.1. NC_000917.1.
    WP_010877997.1. NC_000917.1.

    Genome annotation databases

    EnsemblBacteriaiAAB90747; AAB90747; AF_0490.
    GeneIDi1483707.
    KEGGiafu:AF0490.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000782 Genomic DNA. Translation: AAB90747.1 .
    PIRi B69311.
    RefSeqi NP_069326.1. NC_000917.1.
    WP_010877997.1. NC_000917.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1J2P X-ray 2.60 A/B/C/D/E/F/G 1-246 [» ]
    1J2Q X-ray 2.83 A/B/C/D/E/F/G 10-246 [» ]
    ProteinModelPortali O29760.
    SMRi O29760. Positions 10-246.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O29760. 1 interaction.
    STRINGi 224325.AF0490.

    Protein family/group databases

    MEROPSi T01.973.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB90747 ; AAB90747 ; AF_0490 .
    GeneIDi 1483707.
    KEGGi afu:AF0490.

    Phylogenomic databases

    eggNOGi COG0638.
    KOi K03432.
    OMAi FQVNYAR.

    Enzyme and pathway databases

    BioCyci AFUL224325:GJBC-503-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O29760.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    HAMAPi MF_00289_A. Proteasome_A_A.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR019982. Proteasome_asu_arc.
    IPR000426. Proteasome_asu_N.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SMARTi SM00948. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR03633. arc_protsome_A. 1 hit.
    PROSITEi PS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
      Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
      , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
      Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
    2. "Investigations on the maturation and regulation of archaebacterial proteasomes."
      Groll M., Brandstetter H., Bartunik H., Bourenkow G., Huber R.
      J. Mol. Biol. 327:75-83(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF NATIVE PROTEIN AND IN COMPLEX WITH BETA SUBUNIT AND CALPAIN-INHIBITOR I, SUBUNIT.

    Entry informationi

    Entry nameiPSA_ARCFU
    AccessioniPrimary (citable) accession number: O29760
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3