O29758 (ECR1_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable exosome complex RNA-binding protein 1

Gene names

Ordered Locus Names:

AF_0492

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in degradation of a variety of RNA species; could act a RNA-binding component of the exosome Potential. HAMAP MF_00623
Subunit structure	Component of the archaeal exosome multienzyme ribonuclease complex Potential.
Subcellular location	Cytoplasm Potential HAMAP MF_00623.
Sequence similarities	Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm Exosome
Ligand	RNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell exosome (RNase complex) Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 223

223

Probable exosome complex RNA-binding protein 1 HAMAP MF_00623

PRO_0000050145

Regions

Domain

58 – 127

70

S1 motif

Domain

135 – 193

59

KH

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

223

Helix Strand Turn

Details...

Beta strand

10 – 13

4

Beta strand

22 – 25

4

Beta strand

28 – 31

4

Beta strand

33 – 39

7

Beta strand

44 – 48

5

Beta strand

50 – 52

3

Beta strand

60 – 68

9

Beta strand

70 – 76

7

Beta strand

78 – 81

4

Beta strand

83 – 86

4

Helix

87 – 89

3

Helix

99 – 101

3

Beta strand

108 – 116

9

Beta strand

122 – 125

4

Beta strand

128 – 130

3

Beta strand

132 – 141

10

Helix

144 – 146

3

Helix

147 – 151

5

Helix

153 – 155

3

Helix

156 – 165

10

Beta strand

168 – 171

4

Beta strand

175 – 181

7

Helix

183 – 198

16

Helix

205 – 218

14

Sequences

	Sequence		Length	Mass (Da)
	O29758 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A11B7F1A26972833	FASTA	223	24,725
	10 20 30 40 50 60 MRKIVLPGDL LSTNPRAAGY GTYVEGGKVY AKIIGLFDQT ETHVRVIPLK GRYTPSVGDV 70 80 90 100 110 120 VIGIIREVAA NGWAVDIYSP YQAFLPVSEN PEMKPNKKPN EVLDIGDAII AKVLNIDPKM 130 140 150 160 170 180 KVTLTMKDRI CRPIRFGRIV AINPARVPRV IGKKGSMIKL LKSELDVQIV VGQNGLIWVN 190 200 210 220 GDRRKVSIAE EAIYLIEQEA HTEGLTDRVA EFIKRRKADV GIQ

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000782 Genomic DNA. Translation: AAB90745.1.

PIR

D69311.

RefSeq

NP_069328.1. NC_000917.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BA0	X-ray	2.70	A/B/C	1-223	[»]

ProteinModelPortal

O29758.

SMR

O29758. Positions 2-223.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1483709.

GenomeReviews

Gene locus AF_0492 in contig AE000782_GR.

KEGG

afu:AF0492.

NMPDR

fig|224325.1.peg.487.

TIGR

AF_0492.

Phylogenomic databases

HOGENOM

HBG392276.

OMA

GQNGRIW.

PhylomeDB

O29758.

ProtClustDB

PRK04163.

Enzyme and pathway databases

BioCyc

AFUL224325:AF_0492-MONOMER.

Family and domain databases

HAMAP

MF_00623. Exosome_RNA-bind1.
[Tree]

InterPro

IPR023474. Exosome_RNA_bind1.
IPR004087. KH.
IPR004088. KH_type_1.
IPR018111. KH_type_1_subgr.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR022967. RNA-binding_domain_S1.
[Graphical view]

Gene3D

G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.

KO

K03679.

Pfam

PF00013. KH_1. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]

SMART

SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]

SUPFAM

SSF50249. Nucleic_acid_OB. 1 hit.

PROSITE

PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ECR1_ARCFU

Accession

Primary (citable) accession number: O29758

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program