O29756 (ECX2_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable exosome complex exonuclease 2
EC=3.1.13.-

Gene names

Ordered Locus Names:

AF_0494

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in the 3'->5' degradation of a variety of RNA species Potential. HAMAP MF_00622
Subunit structure	Component of the archaeal exosome multienzyme ribonuclease complex Potential.
Subcellular location	Cytoplasm Potential HAMAP MF_00622.
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Cellular component	Cytoplasm Exosome
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA catabolic process Inferred from electronic annotation. Source: InterPro RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell exosome (RNase complex) Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 259

259

Probable exosome complex exonuclease 2 HAMAP MF_00622

PRO_0000139996

Secondary structure

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259

Helix Strand Turn

Details...

Helix

7 – 18

12

Turn

19 – 21

3

Beta strand

35 – 39

5

Beta strand

45 – 53

9

Beta strand

56 – 67

12

Beta strand

78 – 84

7

Turn

87 – 89

3

Beta strand

95 – 97

3

Helix

100 – 115

16

Helix

121 – 124

4

Beta strand

125 – 127

3

Turn

128 – 130

3

Beta strand

131 – 143

13

Helix

148 – 160

13

Helix

168 – 170

3

Beta strand

185 – 192

8

Beta strand

195 – 199

5

Helix

204 – 207

4

Beta strand

211 – 216

6

Beta strand

222 – 230

9

Helix

235 – 252

18

Turn

253 – 256

4

Sequences

	Sequence		Length	Mass (Da)
	O29756 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: E8289D46F9DDCCB3	FASTA	259	28,647
	10 20 30 40 50 60 MPEDILVDIK RDYVLSKLRD NERIDGRGFD EFRKVEIIPN VIEKAEGSAL VKLGDTQVVV 70 80 90 100 110 120 GVKMQPGEPY PDTPDRGVII VNAELVPLAS PTFEPGPPDE NSIELARVVD RGIRESEAVD 130 140 150 160 170 180 LSKLVIEEGE KVWIVFVDIH ALDDDGNLLD ASALAAIAAL MNTKVPAERF DLGEDYLLPV 190 200 210 220 230 240 RDLPVSVTSL IVGNKYLVDP SREEMSVGDT TLTITTDKDD NVVAMQKSGG YLLDEKLFDE 250 LLDVSINCAR KLREKFKEI

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

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Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000782 Genomic DNA. Translation: AAB90743.1.

PIR

F69311.

RefSeq

NP_069330.1. NC_000917.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BA0	X-ray	2.70	G/H/I	1-259	[»]
2BA1	X-ray	2.70	G/H/I	1-259	[»]
3M7N	X-ray	2.40	G/H/I	1-259	[»]
3M85	X-ray	3.00	G/H/I	1-259	[»]

ProteinModelPortal

O29756.

SMR

O29756. Positions 3-258.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1483711.

GenomeReviews

Gene locus AF_0494 in contig AE000782_GR.

KEGG

afu:AF0494.

NMPDR

fig|224325.1.peg.489.

TIGR

AF_0494.

Phylogenomic databases

HOGENOM

HBG737187.

OMA

FIDVHVL.

PhylomeDB

O29756.

ProtClustDB

PRK04282.

Enzyme and pathway databases

BioCyc

AFUL224325:AF_0494-MONOMER.

Family and domain databases

HAMAP

MF_00622. Exosome_Exonucl_2.
[Tree]

InterPro

IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR020869. Exosome_complex_exonuc_2_prob.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]

KO

K12589.

Pfam

PF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]

SUPFAM

SSF55666. 3_ExoRNase. 1 hit.
SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ECX2_ARCFU

Accession

Primary (citable) accession number: O29756

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program