Reviewed, UniProtKB/Swiss-Prot O29720 (GYRB_ARCFU)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit B
    EC=5.99.1.3

Gene names

Name:	gyrB
Ordered Locus Names:	AF_0530

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	632 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis.
Sequence similarities	Belongs to the type II topoisomerase family.
Sequence caution	The sequence AAB90708.1 differs from that shown. Reason: Frameshift at position 156.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 632	632	DNA gyrase subunit B		PRO_0000145360

Sequences

	Sequence		Length	Mass (Da)
	O29720-1 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 827B59AED94444C0	FASTA	632	71,060
	10 20 30 40 50 60 MSPDQTAVEY TAKDIEVLDD LEAVRKRPGM YIGGVGVRGL HHLLWEVVDN AVDEALAGYC 70 80 90 100 110 120 DTIKVSLHKD GSASVEDNGR GIPTEMHELG KSALEIVMTK LHAGGKFSKK VYRVSGGLHG 130 140 150 160 170 180 VGVSVVNALS EWLEVWVKRN GKIYYQKYQR GSRSPEAGHR TEVLGETKEH GTTVRFKPDR 190 200 210 220 230 240 EIFETTEFKY EIVAQRLKEL AYLNRGLKII LFDEREGKEE TFHFEDGIIG LVRSLNRNRK 250 260 270 280 290 300 PLHEPIYIET TKDGVSVEVA IQFTDSDVEN IQAFANNINT SEGGSHVVGF RAGLTRAVNE 310 320 330 340 350 360 YGKKHLKKFE PVTGVDIREG LTAVISVKVP EPQFEGQTKT KLTNSDVKTV VESAVYSGVL 370 380 390 400 410 420 RWLEENPAQA ETLLNKFILN KKAREAAKRA KELVKRKNEL ITTLPGKLAD CSSKNPEERE 430 440 450 460 470 480 LFIVEGESAG GSAKQARDRR FQAILPIKGK IINVEKAGMA RVLKNDEIKA IISAIGAGIG 490 500 510 520 530 540 KDFDITKARY RRIIIMTDAD VDGAHIRTLL LTFFYRYMRP LIESGYLYIA QPPLYQIKKG 550 560 570 580 590 600 KKSYYAYSDE ELKRTLEQVG GGEVQRYKGL GEMNPQQLWE TTMNPENRIL IQVTLEDAKR 610 620 630 ADELFSILMG EDVESRRNFI MAHSKEVKNL DI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
[2]	Medigue C., Bocs S. Unpublished observations (APR-2001) Cited for: IDENTIFICATION OF PROBABLE FRAMESHIFT.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB90708.1. Frameshift.
PIR	B69316.
RefSeq	NP_069366.1.
3D structure databases
HSSP	HSSP built from PDB template 1AJ6 based on UniProtKB P06982.
ModBase	Search...
Genome annotation databases
GeneID	1483747.
GenomeReviews	Gene locus AF_0530 in contig AE000782_GR.
KEGG	afu:AF0530.
TIGR	AF_0530.
Phylogenomic databases
HOGENOM	O29720.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_0530-MON.
BRENDA	5.99.1.3. 7576.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR011557. GyrB. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA_B/N. IPR013759. Topo_IIA_B/N_ab. IPR013506. Topo_IIA_B_2. IPR002288. Topo_IIA_B_C. IPR018522. TopoIIA_CS. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
Pfam	PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
ProDom	PD149633. DNA_gyrase_B. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01059. gyrB. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRB_ARCFU

Accession

Primary (citable) accession number: O29720

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	September 22, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)