O29703 (SYT_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	AF_0548

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	Threonine--tRNA ligase HAMAP MF_00184		PRO_0000101097
Regions
	Region	196 – 495	300	Catalytic HAMAP MF_00184
Sites
	Metal binding	288	1	Zinc; catalytic By similarity
	Metal binding	340	1	Zinc; catalytic By similarity
	Metal binding	464	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	O29703 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 839AB5D2EE545A02	FASTA	619	72,150
	10 20 30 40 50 60 MKLLLIHADY MEYEVKKKTK LAEPFDGKGE RVEEVLVAFT SVEKGDDENV VRKAAEAIRE 70 80 90 100 110 120 VAEKVNAERI MIYPYAHLSS NLADAETAVK LLKQLEAELS DFEVHRSPFG WYKAFRISCK 130 140 150 160 170 180 GHPLSELSRE IGGEAEAEVT QALKDEEEKV VSYWYILTPE KELVEVEKFD FTGYEKLRKF 190 200 210 220 230 240 VNYEIAKRRA VDVTPPHVEY MRRLELADYE PASDSGHIRY YPKGRLVKTL LEQFITRKCI 250 260 270 280 290 300 DYGAMEVETP IMYDRNHPTL RRYLERFPAR QYIIKGDKRE FFLRFAACFG QFLMLSSSTI 310 320 330 340 350 360 TYRNLPLKIY ELTRYSFRKE QRGELVGLRR LRAFTMPDMH TVAKDMEQAK EEFFNQYRLS 370 380 390 400 410 420 VEVLREIGLE PEDYEVAVRI TKDFYEENRE FVHSLVDILK KPILIEMWDH RFFYFVLKFE 430 440 450 460 470 480 FNFVDALDKA SALSTVQIDV ENAERYGITF VDSDGKEKHP YILHCSVSGA VERVMYALLE 490 500 510 520 530 540 KAKFMLDEGR LPMLPVWLSP TQVRVIPVSE RFVDAAIKIA DDIARNGIRV DVDDRNETLG 550 560 570 580 590 600 KKIRDAQTEW IPYIAVVGEK EIESGKLAVT VRAESTQKEQ KRVEMSAEEL AERVRSECEG 610 KPFMPLPLPK LLSLRPSFR

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB90685.1.
PIR	D69318.
RefSeq	NP_069384.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O29703.
SMR	O29703. Positions 1-134.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1483764.
GenomeReviews	Gene locus AF_0548 in contig AE000782_GR.
KEGG	afu:AF0548.
NMPDR	fig|224325.1.peg.543.
TIGR	AF_0548.
Phylogenomic databases
HOGENOM	HBG392335.
OMA	ETPVMYD.
ProtClustDB	PRK03991.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_0548-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002320. Thr-tRNA-synth_IIa. IPR015011. Threonyl-tRNA_syn_edit_dom_arc. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF08915. tRNA-Thr_ED. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
ProDom	PD016189. Threonyl-tRNA_syntase_arc. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF52954. Anticodon_bd. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_ARCFU

Accession

Primary (citable) accession number: O29703

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program