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O29689

- ISCS2_ARCFU

UniProt

O29689 - ISCS2_ARCFU

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Protein
Cysteine desulfurase IscS 2
Gene
iscS2, AF_0564
Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the removal of elemental sulfur from cysteine to produce alanine Inferred. Binds 1 2Fe-2S cluster per subunit in a crystal formed under reducing conditions; this subunit provides 1 ligand while IscU provides the other 3 ligands. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters.2 Publications

Catalytic activityi

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.UniRule annotation

Cofactori

Pyridoxal phosphate. Cofactor affinity is increased in an IscS-IscU complex.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei70 – 701Pyridoxal phosphate
Binding sitei149 – 1491Pyridoxal phosphate
Binding sitei234 – 2341Pyridoxal phosphate
Active sitei321 – 3211Cysteine persulfide intermediate Inferred
Metal bindingi321 – 3211Iron-sulfur (2Fe-2S)

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. cysteine desulfurase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. cysteine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-577-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine desulfurase IscS 2 (EC:2.8.1.7)
Gene namesi
Name:iscS2
Ordered Locus Names:AF_0564
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
ProteomesiUP000002199: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi321 – 3211C → S: No iron-sulfur cluster assembly. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382Cysteine desulfurase IscS 2UniRule annotation
PRO_0000150288Add
BLAST

Interactioni

Subunit structurei

Homodimer. Forms a heterotetramer with IscU.1 Publication

Protein-protein interaction databases

STRINGi224325.AF0564.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni6 – 83
Helixi14 – 2512
Helixi36 – 5520
Beta strandi61 – 688
Helixi69 – 8416
Helixi85 – 873
Beta strandi90 – 945
Helixi99 – 11012
Beta strandi114 – 1185
Helixi128 – 1347
Beta strandi139 – 1435
Turni149 – 1513
Helixi157 – 1648
Beta strandi165 – 1739
Turni175 – 1773
Helixi185 – 1884
Beta strandi191 – 1977
Helixi198 – 2003
Beta strandi207 – 2126
Helixi227 – 2293
Helixi237 – 27236
Beta strandi278 – 2803
Beta strandi284 – 2874
Beta strandi291 – 2966
Helixi301 – 31010
Helixi320 – 3223
Beta strandi323 – 3253
Helixi330 – 3345
Helixi339 – 3435
Beta strandi345 – 3495
Helixi356 – 37520

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4EB5X-ray2.53A/B1-382[»]
4EB7X-ray2.75A/B1-382[»]
4HVKX-ray1.43A1-382[»]
ProteinModelPortaliO29689.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1104.
KOiK04487.
OMAiALKGLYW.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00331. Cys_desulf_IscS.
InterProiIPR000192. Aminotrans_V/Cys_dSase.
IPR010240. Cys_deSase.
IPR016454. Cysteine_dSase_NifS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

O29689-1 [UniParc]FASTAAdd to Basket

« Hide

MAYFDYTSAK PVDERVLEAM LPYMTESFGN PSSVHSYGFK AREAVQEARE    50
KVAKLVNGGG GTVVFTSGAT EANNLAIIGY AMRNARKGKH ILVSAVEHMS 100
VINPAKFLQK QGFEVEYIPV GKYGEVDVSF IDQKLRDDTI LVSVQHANNE 150
IGTIQPVEEI SEVLAGKAAL HIDATASVGQ IEVDVEKIGA DMLTISSNDI 200
YGPKGVGALW IRKEAKLQPV ILGGGQENGL RSGSENVPSI VGFGKAAEIT 250
AMEWREEAER LRRLRDRIID NVLKIEESYL NGHPEKRLPN NVNVRFSYIE 300
GESIVLSLDM AGIQASTGSA CSSKTLQPSH VLMACGLKHE EAHGTLLLTL 350
GRYNTDEDVD RLLEVLPGVI ERLRSMSPLY RR 382
Length:382
Mass (Da):41,824
Last modified:January 1, 1998 - v1
Checksum:i3B2A25229124C02A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000782 Genomic DNA. Translation: AAB90671.1.
PIRiD69320.
RefSeqiNP_069398.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90671; AAB90671; AF_0564.
GeneIDi1483780.
KEGGiafu:AF0564.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000782 Genomic DNA. Translation: AAB90671.1 .
PIRi D69320.
RefSeqi NP_069398.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4EB5 X-ray 2.53 A/B 1-382 [» ]
4EB7 X-ray 2.75 A/B 1-382 [» ]
4HVK X-ray 1.43 A 1-382 [» ]
ProteinModelPortali O29689.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224325.AF0564.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB90671 ; AAB90671 ; AF_0564 .
GeneIDi 1483780.
KEGGi afu:AF0564.

Phylogenomic databases

eggNOGi COG1104.
KOi K04487.
OMAi ALKGLYW.

Enzyme and pathway databases

BioCyci AFUL224325:GJBC-577-MONOMER.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPi MF_00331. Cys_desulf_IscS.
InterProi IPR000192. Aminotrans_V/Cys_dSase.
IPR010240. Cys_deSase.
IPR016454. Cysteine_dSase_NifS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF00266. Aminotran_5. 1 hit.
[Graphical view ]
PIRSFi PIRSF005572. NifS. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus."
    Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G.
    , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
    Nature 390:364-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
  2. "(IscS-IscU)2 complex structures provide insights into Fe2S2 biogenesis and transfer."
    Marinoni E.N., de Oliveira J.S., Nicolet Y., Raulfs E.C., Amara P., Dean D.R., Fontecilla-Camps J.C.
    Angew. Chem. Int. Ed. 51:5439-5442(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.53 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR (2FE-2S) UNDER REDUCING AND OXIDIZING CONDITIONS, FUNCTION, COFACTOR, SUBUNIT.
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.
  3. "Crystal structure and functional studies of an unusual L-cysteine desulfurase from Archaeoglobus fulgidus."
    Yamanaka Y., Zeppieri L., Nicolet Y., Marinoni E.N., de Oliveira J.S., Odaka M., Dean D.R., Fontecilla-Camps J.C.
    Dalton Trans. 42:3092-3099(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.43 ANGSTROMS), FUNCTION, COFACTOR, PROBABLE ACTIVE SITE, MUTAGENESIS OF CYS-321.
    Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Entry informationi

Entry nameiISCS2_ARCFU
AccessioniPrimary (citable) accession number: O29689
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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