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Protein

Cysteine desulfurase IscS 2

Gene

iscS2

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the removal of elemental sulfur from cysteine to produce alanine (Probable). Binds 1 2Fe-2S cluster per subunit in a crystal formed under reducing conditions; this subunit provides 1 ligand while IscU provides the other 3 ligands. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters.2 Publications

Catalytic activityi

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation2 PublicationsNote: Cofactor affinity is increased in an IscS-IscU complex.2 Publications

Pathwayi: iron-sulfur cluster biosynthesis

This protein is involved in the pathway iron-sulfur cluster biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway iron-sulfur cluster biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70Pyridoxal phosphateUniRule annotation2 Publications1
Binding sitei149Pyridoxal phosphateUniRule annotation2 Publications1
Binding sitei234Pyridoxal phosphateUniRule annotation1 Publication1
Active sitei321Cysteine persulfide intermediateUniRule annotation1 Publication1
Metal bindingi321Iron-sulfur (2Fe-2S); via persulfide group; shared with IscUUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi2.8.1.7. 414.
UniPathwayiUPA00266.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine desulfurase IscS 21 PublicationUniRule annotation (EC:2.8.1.7UniRule annotation)
Gene namesi
Name:iscS2UniRule annotation
Ordered Locus Names:AF_0564
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi321C → S: No iron-sulfur cluster assembly. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001502881 – 382Cysteine desulfurase IscS 2Add BLAST382

Interactioni

Subunit structurei

Forms a heterotetramer with IscU, interacts with other sulfur acceptors.UniRule annotation2 Publications

Protein-protein interaction databases

STRINGi224325.AF0564.

Structurei

Secondary structure

1382
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni6 – 8Combined sources3
Helixi14 – 25Combined sources12
Helixi36 – 55Combined sources20
Beta strandi61 – 68Combined sources8
Helixi69 – 84Combined sources16
Helixi85 – 87Combined sources3
Beta strandi90 – 94Combined sources5
Helixi99 – 110Combined sources12
Beta strandi114 – 118Combined sources5
Helixi128 – 134Combined sources7
Beta strandi139 – 143Combined sources5
Turni149 – 151Combined sources3
Helixi157 – 164Combined sources8
Beta strandi165 – 173Combined sources9
Turni175 – 177Combined sources3
Helixi185 – 188Combined sources4
Beta strandi191 – 197Combined sources7
Helixi198 – 200Combined sources3
Beta strandi207 – 212Combined sources6
Helixi227 – 229Combined sources3
Helixi237 – 272Combined sources36
Beta strandi278 – 280Combined sources3
Beta strandi284 – 287Combined sources4
Beta strandi291 – 296Combined sources6
Helixi301 – 310Combined sources10
Helixi320 – 322Combined sources3
Beta strandi323 – 325Combined sources3
Helixi330 – 334Combined sources5
Helixi339 – 343Combined sources5
Beta strandi345 – 349Combined sources5
Helixi356 – 375Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EB5X-ray2.53A/B1-382[»]
4EB7X-ray2.75A/B1-382[»]
4HVKX-ray1.43A1-382[»]
4R5FX-ray1.90A1-382[»]
ProteinModelPortaliO29689.
SMRiO29689.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00066. Archaea.
COG1104. LUCA.
KOiK04487.
OMAiEHHSILH.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00331. Cys_desulf_IscS. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR010240. Cys_deSase_IscS.
IPR016454. Cysteine_dSase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

O29689-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYFDYTSAK PVDERVLEAM LPYMTESFGN PSSVHSYGFK AREAVQEARE
60 70 80 90 100
KVAKLVNGGG GTVVFTSGAT EANNLAIIGY AMRNARKGKH ILVSAVEHMS
110 120 130 140 150
VINPAKFLQK QGFEVEYIPV GKYGEVDVSF IDQKLRDDTI LVSVQHANNE
160 170 180 190 200
IGTIQPVEEI SEVLAGKAAL HIDATASVGQ IEVDVEKIGA DMLTISSNDI
210 220 230 240 250
YGPKGVGALW IRKEAKLQPV ILGGGQENGL RSGSENVPSI VGFGKAAEIT
260 270 280 290 300
AMEWREEAER LRRLRDRIID NVLKIEESYL NGHPEKRLPN NVNVRFSYIE
310 320 330 340 350
GESIVLSLDM AGIQASTGSA CSSKTLQPSH VLMACGLKHE EAHGTLLLTL
360 370 380
GRYNTDEDVD RLLEVLPGVI ERLRSMSPLY RR
Length:382
Mass (Da):41,824
Last modified:January 1, 1998 - v1
Checksum:i3B2A25229124C02A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90671.1.
PIRiD69320.
RefSeqiWP_010878069.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB90671; AAB90671; AF_0564.
GeneIDi1483780.
KEGGiafu:AF_0564.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90671.1.
PIRiD69320.
RefSeqiWP_010878069.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EB5X-ray2.53A/B1-382[»]
4EB7X-ray2.75A/B1-382[»]
4HVKX-ray1.43A1-382[»]
4R5FX-ray1.90A1-382[»]
ProteinModelPortaliO29689.
SMRiO29689.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF0564.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90671; AAB90671; AF_0564.
GeneIDi1483780.
KEGGiafu:AF_0564.

Phylogenomic databases

eggNOGiarCOG00066. Archaea.
COG1104. LUCA.
KOiK04487.
OMAiEHHSILH.

Enzyme and pathway databases

UniPathwayiUPA00266.
BRENDAi2.8.1.7. 414.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00331. Cys_desulf_IscS. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR010240. Cys_deSase_IscS.
IPR016454. Cysteine_dSase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiISCS2_ARCFU
AccessioniPrimary (citable) accession number: O29689
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In Archaea the pyridoxal phosphate cofactor is not covalently bound to Lys but ligated by other amino acids.UniRule annotation

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.