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O29632 (DNLI_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene names
Name:lig
Ordered Locus Names:AF_0623
OrganismArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) [Reference proteome] [HAMAP]
Taxonomic identifier224325 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus

Protein attributes

Sequence length555 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence caution

The sequence AAB90616.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 555555DNA ligase HAMAP-Rule MF_00407
PRO_0000059601

Sites

Active site2491N6-AMP-lysine intermediate By similarity
Binding site2471ATP By similarity
Binding site2541ATP By similarity
Binding site2691ATP By similarity
Binding site2981ATP By similarity
Binding site3371ATP By similarity
Binding site4111ATP By similarity
Binding site4171ATP By similarity

Secondary structure

......................................................................................... 555
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O29632 [UniParc].

Last modified June 20, 2001. Version 2.
Checksum: D050FE80B2341EDB

FASTA55563,553
        10         20         30         40         50         60 
MLFAEFAEFC ERLEKISSTL ELTARIAAFL QKIEDERDLY DVVLFITGKV YPPWDERELG 

        70         80         90        100        110        120 
VGIGLLYEAL ENVSGVKRSE IESMIREYGD LGLVAEQLIK KKKMTTLAFE ELTVRKVRET 

       130        140        150        160        170        180 
FDEIASLTGE GSMKRKIMLL TGLYGLATPL EARYLTRLIL NEMRLGVGEG IMRDAIARAF 

       190        200        210        220        230        240 
RADPETVERA YMITNDLGRV AVVAKKEGEE GLRKMKIEIH IPVRMMLAQV AESLESAVRE 

       250        260        270        280        290        300 
MRTAAVEWKF DGSRVQVHWD GSRVTIYSRR LENVTNALPD IVEEIKKSVK PGVILDGEVI 

       310        320        330        340        350        360 
AVKEGKPMPF QHVLRRFRRK HDVAKMVEKI PLEAHFFDIL YHDGECIDLP LRERRKLLES 

       370        380        390        400        410        420 
AVNESEKIKL AKQIVTDSVD EVRKMYDEAI SAGHEGVMIK LPSSPYIPGK RGKNWLKVKA 

       430        440        450        460        470        480 
IMETLDLVVV GGEWGEGKRS HWLSSFELAC LDPVTGKLLK VGRVATGFTE EDLEELTEMF 

       490        500        510        520        530        540 
RPLIVSQQGK KVEFIPKYVF EVAYQEIQKS PKYESGYALR FPRFVRLRDD KDVDEADTIE 

       550 
RVENLYKLQF EVKRQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000782 Genomic DNA. Translation: AAB90616.1. Different initiation.
PIRG69327.
RefSeqNP_069457.1. NC_000917.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3GDEX-ray2.30A1-555[»]
ProteinModelPortalO29632.
SMRO29632. Positions 1-554.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224325.AF0623.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB90616; AAB90616; AF_0623.
GeneID1483841.
KEGGafu:AF0623.

Phylogenomic databases

eggNOGCOG1793.
KOK10747.
OMAARVQVHK.

Enzyme and pathway databases

BioCycAFUL224325:GJBC-638-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO29632.

Entry information

Entry nameDNLI_ARCFU
AccessionPrimary (citable) accession number: O29632
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 20, 2001
Last modified: May 14, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references