O29627 (LEU3_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-isopropylmalate dehydrogenase
Short name=3-IPM-DH
Short name=IMDH
EC=1.1.1.85

Alternative name(s):
Beta-IPM dehydrogenase

Gene names

Name:	leuB
Ordered Locus Names:	AF_0628

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	3-isopropylmalate dehydrogenase		PRO_0000083808
Regions
	Nucleotide binding	255 – 267	13	NAD By similarity
Sites
	Metal binding	198	1	Magnesium or manganese By similarity
	Metal binding	222	1	Magnesium or manganese By similarity
	Metal binding	226	1	Magnesium or manganese By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	91	1	Substrate By similarity
	Binding site	112	1	Substrate By similarity
	Binding site	198	1	Substrate By similarity
	Site	119	1	Important for catalysis By similarity
	Site	165	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	O29627 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 4B3BE727572CF0A9	FASTA	326	35,819
	10 20 30 40 50 60 MKKIVVIPGD GIGKEVMEAA MLILEKLDLP FEYSYYDAGD EALEKYGKAL PDETLEACRK 70 80 90 100 110 120 SDAVLFGAAG ETAADVIVRL RRELGTFANV RPAKAIEGIE CLYPGLDIVV VRENTECLYM 130 140 150 160 170 180 GFEFGFGDVT EAIRVITREA SERIARYAFE LAKREGRKKV TALHKANVMK KTCGLFRDVC 190 200 210 220 230 240 REVAKDYPEI QYNDYYIDAA CMYLVMDPFR FDVIVTTNMF GDIVSDLAAG LVGGLGLAPS 250 260 270 280 290 300 ANVGERTAIF EPVHGAAFDI AGKGIANPTA MILTACMMLR HFGYVEEAKK VEEAVEKTIK 310 320 EGKKTPDLGG NLKTMEFANE VASLLD

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB90611.1.
PIR	D69328.
RefSeq	NP_069462.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O29627.
SMR	O29627. Positions 2-325.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1483846.
GenomeReviews	Gene locus AF_0628 in contig AE000782_GR.
KEGG	afu:AF0628.
NMPDR	fig|224325.1.peg.621.
TIGR	AF_0628.
Phylogenomic databases
HOGENOM	HBG518924.
OMA	TCAHKAN.
PhylomeDB	O29627.
ProtClustDB	PRK14025.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_0628-MONOMER.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR024084. IsoPropMal-DH-like_dom. IPR011828. LEU3_arc. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
KO	K00052.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR02088. LEU3_arch. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_ARCFU

Accession

Primary (citable) accession number: O29627

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program