O29626 (LEUD1_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-isopropylmalate dehydratase small subunit 1
EC=4.2.1.33

Alternative name(s):
Alpha-IPM isomerase 1
Short name=IPMI 1
Isopropylmalate isomerase 1

Gene names

Name:	leuD1
Ordered Locus Names:	AF_0629

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate. HAMAP MF_01032
Catalytic activity	(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmaleate + H2O. HAMAP MF_01032 (2S)-2-isopropylmaleate + H2O = (2S)-2-isopropylmalate. HAMAP MF_01032
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP MF_01032
Subunit structure	Heterodimer of LeuC and LeuD By similarity. HAMAP MF_01032
Sequence similarities	Belongs to the LeuD family. LeuD type 2 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	3-isopropylmalate dehydratase complex Inferred from electronic annotation. Source: InterPro
Molecular function	3-isopropylmalate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	3-isopropylmalate dehydratase small subunit 1 HAMAP MF_01032		PRO_0000141931

Sequences

	Sequence		Length	Mass (Da)
	O29626 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 6DEBE0A2AEDB16A7	FASTA	161	18,137
	10 20 30 40 50 60 MGRAWKFGDD IDTDVIIQGK YLVINEPEEL AKHVFENLRP EFAKEVKKGD FVVAGENFGC 70 80 90 100 110 120 GSSREHAPLA LKATGIEAVI AKSYARIFFR NAINIGLRVL ECKETDKIED GDELEVDYEK 130 140 150 160 GVIYNKTKGE EYPINPLPDF LKEILEKGGL VEFAKSLRER A

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB90610.1.
PIR	E69328.
RefSeq	NP_069463.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O29626.
SMR	O29626. Positions 2-156.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1483847.
GenomeReviews	Gene locus AF_0629 in contig AE000782_GR.
KEGG	afu:AF0629.
NMPDR	fig|224325.1.peg.622.
TIGR	AF_0629.
Phylogenomic databases
HOGENOM	HBG304838.
OMA	CVIAETF.
ProtClustDB	PRK00439.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_0629-MONOMER.
Family and domain databases
HAMAP	MF_01032. LeuD_type2. [Tree]
InterPro	IPR015937. Acoase/IPM_deHydtase. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. IPR011827. IsopropMal_deHydtase_ssu. [Graphical view]
Gene3D	G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit.
KO	K01704.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit.
Pfam	PF00694. Aconitase_C. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR02087. LEUD_arch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEUD1_ARCFU

Accession

Primary (citable) accession number: O29626

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program